| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHT |
| Uniprot No | Q76M96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-950aa |
| 氨基酸序列 | MTWRMGPRFTMLLAMWLVCGSEPHPHATIRGSHGGRKVPLVSPDSSRPARFLRHTGRSRGIERSTLEEPNLQPLQRRRSVPVLRLARPTEPPARSDINGAAVRPEQRPAARGSPREMIRDEGSSARSRMLRFPSGSSSPNILASFAGKNRVWVISAPHASEGYYRLMMSLLKDDVYCELAERHIQQIVLFHQAGEEGGKVRRITSEGQILEQPLDPSLIPKLMSFLKLEKGKFGMVLLKKTLQVEERYPYPVRLEAMYEVIDQGPIRRIEKIRQKGFVQKCKASGVEGQVVAEGNDGGGGAGRPSLGSEKKKEDPRRAQVPPTRESRVKVLRKLAATAPALPQPPSTPRATTLPPAPATTVTRSTSRAVTVAARPMTTTAFPTTQRPWTPSPSHRPPTTTEVITARRPSVSENLYPPSRKDQHRERPQTTRRPSKATSLESFTNAPPTTISEPSTRAAGPGRFRDNRMDRREHGHRDPNVVPGPPKPAKEKPPKKKAQDKILSNEYEEKYDLSRPTASQLEDELQVGNVPLKKAKESKKHEKLEKPEKEKKKKMKNENADKLLKSEKQMKKSEKKSKQEKEKSKKKKGGKTEQDGYQKPTNKHFTQSPKKSVADLLGSFEGKRRLLLITAPKAENNMYVQQRDEYLESFCKMATRKISVITIFGPVNNSTMKIDHFQLDNEKPMRVVDDEDLVDQRLISELRKEYGMTYNDFFMVLTDVDLRVKQYYEVPITMKSVFDLIDTFQSRIKDMEKQKKEGIVCKEDKKQSLENFLSRFRWRRRLLVISAPNDEDWAYSQQLSALSGQACNFGLRHITILKLLGVGEEVGGVLELFPINGSSVVEREDVPAHLVKDIRNYFQVSPEYFSMLLVGKDGNVKSWYPSPMWSMVIVYDLIDSMQLRRQEMAIQQSLGMRCPEDEYAGYGYHSYHQGYQDGYQDDYRHHESYHHGYPY |
| 预测分子量 | 108 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为假设性示例的参考文献格式(仅供示例，实际引用请核实真实文献)：
1. **《Production of Recombinant DHT-Binding Protein in E. coli》**
作者：J. Smith et al.
摘要：研究利用大肠杆菌系统高效表达重组DHT结合蛋白，优化纯化流程并验证其与双氢睾酮的特异性结合能力。
2. **《Structural Analysis of Recombinant Androgen Receptor Ligand Domain》**
作者：L. Chen & M. Tanaka
摘要：通过X射线晶体学解析重组雄激素受体配体结合域与DHT的复合物结构，揭示DHT诱导受体构象变化的分子机制。
3. **《Recombinant DHT-Modified Proteins in Androgenetic Alopecia Therapy》**
作者：R. Gupta et al.
摘要：开发基于重组DHT修饰蛋白的靶向递送系统，评估其在抑制雄激素性脱发中的潜在治疗效果。
4. **《Functional Characterization of Recombinant 5α-Reductase Isozymes》**
作者：K. Park & S. Yamamoto
摘要：对比重组表达的人源5α-还原酶同工酶活性，阐明其在睾酮向DHT转化过程中的动力学差异及抑制剂筛选应用。
注：以上文献名为虚构示例，实际研究需参考PubMed、ScienceDirect等平台收录的权威论文。
**Background of DHT Recombinant Protein**
Dihydrotestosterone (DHT), a potent androgen derived from testosterone via 5α-reductase enzyme activity, plays critical roles in male sexual development, prostate growth, and androgen-dependent conditions such as androgenetic alopecia and benign prostatic hyperplasia. Recombinant DHT protein refers to a bioengineered form of DHT produced using recombinant DNA technology, enabling scalable and consistent synthesis for research and therapeutic applications.
Traditional DHT extraction from natural sources is limited by low yield, ethical concerns, and batch variability. Recombinant technology overcomes these challenges by inserting genes encoding enzymes involved in DHT synthesis (e.g., 5α-reductase) into host systems like *E. coli*, yeast, or mammalian cells. These systems are optimized to produce high-purity DHT with structural and functional equivalence to endogenous hormone, ensuring reproducibility in preclinical studies.
DHT recombinant protein is widely used to investigate androgen receptor (AR) signaling, hormone-dependent cancers, and metabolic pathways. In drug development, it serves as a reference compound for testing anti-androgens or 5α-reductase inhibitors. Additionally, it aids in studying conditions like polycystic ovary syndrome (PCOS) or disorders of sexual differentiation, where DHT dysregulation is implicated.
Recent advancements focus on improving production efficiency, stability, and delivery systems, such as nanoparticle encapsulation for targeted therapies. Despite progress, challenges remain in mimicking tissue-specific DHT metabolism and minimizing off-target effects. Overall, recombinant DHT represents a vital tool bridging molecular research and clinical innovation in endocrinology and beyond.
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