| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCK8 |
| Uniprot No | P06307 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-115aa |
| 氨基酸序列 | MNSGVCLCVLMAVLAAGALTQPVPPADPAGSGLQRAEEAPRRQLRVSQRTDGESRAHLGALLARYIQQARKAPSGRMSIVKNLQNLDPSHRISDRDYMGWMDFGRRSAEEYEYPS |
| 预测分子量 | 12,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCK8(胆囊收缩素8)重组蛋白的3篇参考文献示例，涵盖表达、功能及应用方向：
1. **文献名称**：*Expression and Purification of Recombinant Cholecystokinin Octapeptide (CCK8) in Escherichia coli*
**作者**：Smith J. et al.
**摘要**：本研究描述了一种在大肠杆菌中高效表达重组CCK8的方法，通过融合标签技术实现可溶性表达，并利用层析技术纯化。生物活性实验证实重组CCK8可激活胰腺细胞膜受体，提示其潜在药理学应用。
2. **文献名称**：*Functional Characterization of Recombinant CCK8 in Neuronal Signaling*
**作者**：Zhang L. et al.
**摘要**：通过体外培养神经元模型，验证了重组CCK8对突触可塑性的调控作用，证明其通过激活CCK2受体增强钙离子内流，为神经退行性疾病研究提供工具。
3. **文献名称**：*Antiproliferative Effects of Recombinant CCK8 in Colorectal Cancer Cells*
**作者**：Wang Y. et al.
**摘要**：研究利用重组CCK8处理结直肠癌细胞，发现其通过诱导细胞周期阻滞和凋亡抑制肿瘤生长，机制涉及ERK信号通路抑制，为癌症治疗提供新思路。
(注：上述文献为示例，实际引用请查询具体数据库获取真实文献。)
**Background of CCK8 Recombinant Protein**
Cholecystokinin-8 (CCK-8), a bioactive octapeptide derived from the larger precursor protein cholecystokinin (CCK), is a critical regulator in gastrointestinal and neuroendocrine systems. Discovered in the 1920s, CCK was initially identified for its role in stimulating gallbladder contraction and pancreatic enzyme secretion. The active form CCK-8. corresponding to the C-terminal eight amino acids of CCK, retains full biological activity and exhibits high affinity for CCK receptors (CCK1R and CCK2R).
CCK-8 functions as both a hormone and a neurotransmitter. In the gut, it regulates digestion, satiety, and gut motility. In the central nervous system, it modulates anxiety, memory, and pain perception. Its dual role underscores its importance in bridging gut-brain communication.
Recombinant CCK-8 is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper post-translational modifications, such as sulfation of tyrosine residues, which is essential for receptor binding. Advances in protein purification and peptide synthesis have enabled high-purity, scalable production of CCK-8 for research and therapeutic applications.
As a research tool, recombinant CCK-8 is widely used to study receptor signaling pathways, screen drug candidates, and explore mechanisms underlying metabolic or neurological disorders. Its clinical potential includes targeting appetite regulation, gastrointestinal disorders, and anxiety-related conditions. The development of CCK-8 analogs further highlights its versatility in biomedical research and drug discovery.
In summary, CCK-8 recombinant protein serves as a vital molecule for understanding gut-brain interactions and developing therapies for diverse physiological and pathological processes.
×
