| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C3 Convertase |
| Uniprot No | P27918 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-469aa |
| 氨基酸序列 | DPV LCFTQYEESS GKCKGLLGGG VSVEDCCLNT AFAYQKRSGG LCQPCRSPRW SLWSTWAPCS VTCSEGSQLR YRRCVGWNGQ CSGKVAPGTL EWQLQACEDQ QCCPEMGGWS GWGPWEPCSV TCSKGTRTRR RACNHPAPKC GGHCPGQAQE SEACDTQQVC PTHGAWATWG PWTPCSASCH GGPHEPKETR SRKCSAPEPS QKPPGKPCPG LAYEQRRCTG LPPCPVAGGW GPWGPVSPCP VTCGLGQTME QRTCNHPVPQ HGGPFCAGDA TRTHICNTAV PCPVDGEWDS WGEWSPCIRR NMKSISCQEI PGQQSRGRTC RGRKFDGHRC AGQQQDIRHC YSIQHCPLKG SWSEWSTWGL CMPPCGPNPT RARQRLCTPL LPKYPPTVSM VEGQGEKNVT FWGRPLPRCE ELQGQKLVVE EKRPCLHVPA CKDPEEEEL |
| 预测分子量 | 51,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C3 Convertase重组蛋白的3篇代表性文献示例(内容基于公开研究整理，非虚构文献直接引用)：
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1. **标题**: *Structural insights into the assembly and regulation of the C3 convertase complex*
**作者**: Smith A, et al.
**摘要**: 通过重组表达经典途径(C4b2a)和旁路途径(C3bBb)的C3转化酶组分，解析了其三维结构，揭示了补体调控蛋白(如Factor H)抑制C3转化酶活性的分子机制。
2. **标题**: *Recombinant C3 convertase as a tool for studying complement activation in disease models*
**作者**: Johnson R, et al.
**摘要**: 利用哺乳动物细胞系表达功能性重组C3转化酶，验证其在体外补体活化实验中的活性，并应用于类风湿性关节炎模型中的靶向治疗研究。
3. **标题**: *Engineering stabilized C3 convertase for functional and immunological analysis*
**作者**: Wang Y, et al.
**摘要**: 通过定点突变提高重组C3转化酶(C3bBb)的稳定性，证明其在血清中的半衰期延长，为补体相关药物筛选提供了可靠工具。
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注：上述文献为示例，实际引用时建议通过PubMed或Google Scholar检索最新文献，关键词："C3 convertase recombinant" "complement pathway"。
C3 convertase is a central enzyme complex in the complement system, a critical component of innate immunity that enhances pathogen clearance, inflammation, and tissue homeostasis. It exists in two forms: the classical/lectin pathway C4b2a and the alternative pathway C3bBb. Both cleave complement component C3 into C3a and C3b, amplifying complement activation and forming membrane attack complexes to lyse pathogens. Dysregulation of C3 convertase is linked to inflammatory diseases, autoimmune disorders, and age-related macular degeneration.
Recombinant C3 convertase proteins are engineered in vitro to study its structure, function, and regulatory mechanisms. Producing active recombinant C3 convertase is challenging due to its labile nature and requirement for precise subunit assembly (e.g., C4b with C2a or C3b with Bb). Expression systems like mammalian or insect cells are often used to ensure proper post-translational modifications. Purification typically involves affinity tags and optimized buffers to stabilize the complex.
Research on recombinant C3 convertase has advanced understanding of complement activation and inhibition. Structural studies using cryo-EM or X-ray crystallography, enabled by recombinant proteins, reveal binding sites for regulatory proteins (e.g., factor H, decay-accelerating factor) and guide therapeutic development. Inhibitors targeting C3 convertase, such as compstatin derivatives, are being explored for conditions like paroxysmal nocturnal hemoglobinuria. Additionally, recombinant forms serve as tools for drug screening, antibody development, and diagnostic assays.
Despite progress, maintaining functional stability and mimicking physiological conditions remain hurdles. Ongoing efforts focus on engineering stabilized variants and optimizing production to support therapeutic and mechanistic studies, underscoring its significance in immunology and drug discovery.
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