| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THRb |
| Uniprot No | P00734 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 44-622aa |
| 氨基酸序列 | ANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE |
| 预测分子量 | 67.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THRb(甲状腺激素受体β)重组蛋白的模拟参考文献示例，供参考：
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1. **文献名称**: "Expression and Purification of Recombinant Human Thyroid Hormone Receptor Beta in Escherichia coli"
**作者**: Zhang Y, et al.
**摘要**: 本研究成功构建了重组人甲状腺激素受体β(THRb)的原核表达系统，利用大肠杆菌进行高效表达，并通过亲和层析纯化获得高纯度蛋白。Western blot及圆二色光谱分析证实其正确折叠及活性，为后续功能研究奠定基础。
2. **文献名称**: "Crystal Structure Analysis of the Ligand-Binding Domain of Thyroid Hormone Receptor Beta"
**作者**: Smith JL, et al.
**摘要**: 通过X射线晶体学解析了THRb配体结合域的三维结构，揭示了甲状腺激素T3与其结合的关键氨基酸残基及构象变化，为设计靶向THRb的调节剂提供结构依据。
3. **文献名称**: "Functional Characterization of Recombinant THRb in Thyroid Hormone Signaling Pathways"
**作者**: Johnson M, et al.
**摘要**: 在哺乳动物细胞中表达重组THRb，证明其可特异性结合甲状腺激素响应元件(TRE)，并调控下游基因转录。通过荧光素酶报告基因实验，验证了THRb在信号通路中的关键作用。
4. **文献名称**: "High-Throughput Screening of THRb Agonists Using a Recombinant Protein-Based Assay"
**作者**: Lee S, et al.
**摘要**: 基于重组THRb蛋白建立高通量筛选平台，鉴定出多个新型小分子激动剂，为甲状腺功能减退症及代谢性疾病的药物开发提供候选化合物。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等学术平台检索真实发表的论文。
**Background of THRb Recombinant Protein**
Thyroid hormone receptor beta (THRβ), a member of the nuclear receptor superfamily, plays a critical role in regulating gene expression mediated by thyroid hormones (T3 and T4). It is essential for maintaining metabolic homeostasis, growth, and development. THRβ exists in two major isoforms, THRβ1 and THRβ2. generated by alternative splicing. These isoforms exhibit tissue-specific expression, with THRβ1 widely distributed in organs like the liver, heart, and brain, while THRβ2 is primarily found in the pituitary and hypothalamus.
Recombinant THRβ proteins are engineered using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, to produce functional, purified receptors for research and therapeutic applications. These proteins retain key structural features, including a DNA-binding domain (DBD) and a ligand-binding domain (LBD), enabling interaction with thyroid hormone response elements (TREs) and binding to thyroid hormones or synthetic ligands.
THRβ recombinant proteins are vital tools for studying thyroid hormone signaling mechanisms, receptor-ligand interactions, and mutations linked to resistance to thyroid hormone (RTH) syndromes. They also facilitate drug discovery, particularly for THRβ-selective agonists targeting metabolic disorders (e.g., non-alcoholic steatohepatitis, dyslipidemia) while minimizing cardiac side effects associated with THRα activation. Additionally, structural studies using recombinant THRβ have advanced the design of isoform-specific therapeutics, underscoring its significance in both basic research and clinical translation.
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