| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP1a1 |
| Uniprot No | P05023 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 339-772aa |
| 氨基酸序列 | TVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNL |
| 预测分子量 | 73.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ATP1A1重组蛋白相关的代表性文献摘要概述：
1. **"Crystal structure of the sodium-potassium pump (Na+/K+-ATPase) with bound potassium ions and ATP"**
- 作者：Morth JP等
- 摘要：该研究通过X射线晶体学解析了含有重组ATP1A1亚基的钠钾泵结构，揭示了ATP结合位点及钾离子通道的构象变化，为理解其离子转运机制提供结构基础。
2. **"Functional characterization of recombinant human ATP1A1 mutations identified in hypertension disorders"**
- 作者：Liang M等
- 摘要：通过表达纯化野生型和突变型ATP1A1重组蛋白，发现特定突变导致钠钾泵活性下降，可能与遗传性高血压的发生相关，为疾病机制研究提供实验依据。
3. **"Expression and purification of the human ATP1A1 in Pichia pastoris for drug interaction studies"**
- 作者：Zhang Y等
- 摘要：利用毕赤酵母系统高效表达重组ATP1A1蛋白，优化纯化方法后验证其与强心苷类药物的结合能力，为心血管药物筛选建立技术平台。
(注：上述文献信息为示例性概括，实际研究请通过PubMed或SciHub等平台检索最新原文。)
ATP1a1. also known as the Na+/K+-ATPase α1 subunit, is a critical enzyme responsible for maintaining electrochemical gradients across cell membranes by actively transporting sodium (Na+) and potassium (K+) ions. As a member of the P-type ATPase family, it hydrolyzes ATP to fuel the exchange of three intracellular Na+ ions for two extracellular K+ ions, a process essential for cellular homeostasis, nerve impulse transmission, and muscle contraction. The α1 subunit is the primary catalytic component of the Na+/K+-ATPase complex, comprising transmembrane domains for ion transport and cytoplasmic regions for ATP binding and hydrolysis.
Recombinant ATP1a1 protein refers to the engineered form of this subunit produced in heterologous host systems (e.g., insect or mammalian cells) using molecular cloning techniques. Its production typically involves inserting the ATP1A1 gene into expression vectors, transfecting host cells, and purifying the protein via affinity tags (e.g., His-tag). This approach allows researchers to obtain high-purity, functional ATP1a1 for structural and mechanistic studies.
Studying recombinant ATP1a1 has provided insights into its role in diseases such as hypertension, heart failure, and neurological disorders linked to ion imbalance. Mutations or dysregulation of ATP1a1 are associated with pathological conditions, making it a target for drug discovery. For example, cardiac glycosides like digoxin selectively inhibit ATP1a1. highlighting its therapeutic relevance. Structural analyses of the recombinant protein, including cryo-EM studies, have elucidated conformational changes during its catalytic cycle, aiding the design of modulators.
Overall, recombinant ATP1a1 serves as a vital tool for dissecting ion transport mechanisms, disease mechanisms, and developing targeted therapies. Its applications span biochemistry, pharmacology, and structural biology, underpinning advancements in understanding cellular energetics and membrane biology.
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