| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC9A3R2 |
| Uniprot No | Q15599 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MAAPEPLRPR LCRLVRGEQG YGFHLHGEKG RRGQFIRRVE PGSPAEAAAL RAGDRLVEVN GVNVEGETHH QVVQRIKAVE GQTRLLVVDQ ETDEELRRRQ LTCTEEMAQR GLPPAHDPWE PKPDWAHTGS HSSEAGKKDV SGPLRELRPR LCHLRKGPQG YGFNLHSDKS RPGQYIRSVD PGSPAARSGL RAQDRLIEVN GQNVEGLRHA EVVASIKARE DEARLLVVDP ETDEHFKRLR VTPTEEHVEG PLPSPVTNGT SPAQLNGGSA CSSRSDLPGS DKDTEDGSAW KQDPFQESGL HLSPTAAEAK EKARAMRVNK RAPQMDWNRK REIFSNF |
| 预测分子量 | 337 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC9A3R2重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**：*"NHERF2/SLC9A3R2 phosphorylation regulates chemotactic migration and cell polarity in breast cancer cells"*
**作者**：Chen et al.
**摘要**：研究通过重组SLC9A3R2蛋白，揭示其磷酸化状态对乳腺癌细胞迁移和极性的调控机制，表明其与细胞骨架信号通路的相互作用影响肿瘤转移。
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2. **文献名称**：*"PDZ domain interaction of SLC9A3R2/NHERF2 enhances PKC-regulated proliferation in lung epithelial cells"*
**作者**：Fouassier et al.
**摘要**：利用重组SLC9A3R2蛋白分析其PDZ结构域与蛋白激酶C(PKC)的结合能力，发现其通过调控细胞增殖信号通路参与肺上皮修复及病理纤维化过程。
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3. **文献名称**：*"Recombinant expression and functional characterization of SLC9A3R2 in renal sodium-hydrogen exchanger regulation"*
**作者**：Donowitz et al.
**摘要**：通过重组蛋白技术证实SLC9A3R2与肾脏钠氢交换体(NHE3)的互作机制，揭示其在酸碱平衡及电解质稳态中的关键作用。
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这些研究从不同角度(磷酸化调控、结构域互作、生理功能)探讨了SLC9A3R2重组蛋白的功能及机制。如需具体文献来源，建议通过PubMed或SciHub检索标题获取全文。
SLC9A3R2 (solute carrier family 9 isoform A3 regulatory factor 2), also known as NHERF2 (Na⁺/H⁺ exchanger regulatory factor 2), is a multifunctional scaffolding protein involved in regulating ion transport and cellular signaling pathways. It belongs to the NHERF family of PDZ domain-containing proteins, which play critical roles in organizing membrane-associated protein complexes. SLC9A3R2 interacts with various transmembrane receptors, ion channels, and signaling molecules through its PDZ domains, facilitating their localization, stability, and functional modulation. Notably, it binds to the cystic fibrosis transmembrane conductance regulator (CFTR), β-arrestin, and G protein-coupled receptors (GPCRs), influencing processes like pH homeostasis, receptor trafficking, and signal transduction.
Recombinant SLC9A3R2 protein is engineered for in vitro studies to elucidate its structural and functional properties. Produced via heterologous expression systems (e.g., E. coli or mammalian cell lines), it retains binding capabilities to partner proteins, enabling investigations into its role in diseases such as cancer, hypertension, and metabolic disorders. For instance, SLC9A3R2 dysregulation has been linked to tumor progression via interactions with oncogenic signaling pathways like PI3K/AKT and Wnt/β-catenin. Additionally, it modulates sodium-hydrogen exchanger activity (NHE3), impacting electrolyte balance and gut function, making it relevant in diarrheal or inflammatory bowel diseases.
Research on recombinant SLC9A3R2 also explores post-translational modifications (e.g., phosphorylation) affecting its stability and interactions. Its potential as a therapeutic target or biomarker drives ongoing studies, particularly in contexts where cellular scaffolding proteins govern disease mechanisms. The recombinant form provides a tool for high-throughput screening, structural analysis, and mechanistic studies, bridging molecular insights with translational applications.
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