| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD11c |
| Uniprot No | P20702 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1163aa |
| 氨基酸序列 | MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK |
| 预测分子量 | 127,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD11c重组蛋白的3篇示例文献(注：文献信息为模拟示例，实际引用需核实真实来源)：
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1. **文献名称**: "Structural and functional characterization of recombinant CD11c integrin αXβ2"
**作者**: Smith A, et al. (2005)
**摘要**: 研究通过重组表达系统获得CD11c蛋白(αXβ2整合素)，解析其晶体结构，并验证其在体外模型中与ICAM-1的结合活性，揭示其在白细胞黏附中的分子机制。
2. **文献名称**: "Expression and purification of bioactive CD11c in mammalian cells for immunotherapy applications"
**作者**: Brown C, et al. (2010)
**摘要**: 开发了基于哺乳动物细胞(HEK293)的重组CD11c表达系统，优化纯化流程获得高纯度蛋白，证实其可增强树突状细胞的抗原呈递功能，为疫苗佐剂开发提供基础。
3. **文献名称**: "CD11c-targeted recombinant protein attenuates inflammation in murine autoimmune encephalomyelitis"
**作者**: Zhang L, et al. (2017)
**摘要**: 构建CD11c重组融合蛋白(CD11c-Fc)，在小鼠多发性硬化模型中证明其通过阻断炎症信号通路显著减轻中枢神经系统炎症损伤，提示其潜在治疗价值。
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如需具体领域(如肿瘤免疫、蛋白互作)的文献，可进一步说明需求。真实文献可通过PubMed或Google Scholar搜索关键词(如“recombinant CD11c protein”)获取。
CD11c, a member of the integrin family, is a transmembrane glycoprotein that pairs with CD18 (β₂ integrin) to form the heterodimeric receptor CR4 (αXβ2 integrin). Primarily expressed on dendritic cells (DCs), macrophages, certain T-cell subsets, and natural killer (NK) cells, it plays a critical role in cell adhesion, migration, and immune signaling. CD11c is widely recognized as a key surface marker for conventional DC subsets, making it essential for studying DC biology and immune regulation.
Recombinant CD11c proteins are engineered to mimic the native structure, typically containing the extracellular domain to preserve ligand-binding functionality. Produced via mammalian or insect expression systems, these proteins ensure proper post-translational modifications, such as glycosylation, which are crucial for stability and activity. Affinity chromatography purification methods (e.g., His-tag or Fc-fusion systems) are employed to achieve high purity and specificity.
In research, recombinant CD11c serves as a vital tool for investigating immune cell interactions, ligand-receptor dynamics (e.g., binding to ICAM-1. fibrinogen, or complement components), and dendritic cell maturation. It is used to develop antibodies, block integrin-mediated pathways in autoimmune or inflammatory disease models, and study tumor microenvironments. Additionally, it acts as a positive control in flow cytometry, Western blotting, and immunohistochemistry to identify CD11c⁺ cells. Its applications extend to vaccine development, cancer immunotherapy, and understanding infections where DCs mediate pathogen clearance. By enabling precise manipulation of CD11c-dependent processes, recombinant CD11c proteins advance both basic immunology and therapeutic discovery.
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