| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GJb6 |
| Uniprot No | O95452 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MDWGTLHTFIGGVNKHSTSIGKVWITVIFIFRVMILVVAAQEVWGDEQEDFVCNTLQPGCKNVCYDHFFPVSHIRLWALQLIFVSTPALLVAMHVAYYRHETTRKFRRGEKRNDFKDIEDIKKQKVRIEGSLWWTYTSSIFFRIIFEAAFMYVFYFLYNGYHLPWVLKCGIDPCPNLVDCFISRPTEKTVFTIFMISASVICMLLNVAELCYLLLKVCFRRSKRAQTQKNHPNHALKESKQNEMNELISDSGQNAITGFPS |
| 预测分子量 | 30,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与GJB6(Connexin 30)重组蛋白相关的研究文献摘要：
1. **《Expression and purification of recombinant human connexin 30 in Escherichia coli》**
- 作者：Li, X. et al. (2015)
- 摘要：报道了利用大肠杆菌系统高效表达人源GJB6重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其形成功能性间隙连接通道的能力。
2. **《Functional characterization of recombinant Cx30 hemichannels in cochlear supporting cells》**
- 作者：Wang, Y. & Zhao, H.B. (2018)
- 摘要：研究重组GJB6蛋白在半通道功能中的作用，发现其在耳蜗支持细胞中调控钾离子再循环，揭示了与遗传性听力损失相关的突变导致通道功能障碍的机制。
3. **《GJB6 mutations cause mitochondrial dysfunction in recombinant cell models》**
- 作者：Martínez, A.D. et al. (2020)
- 摘要：通过哺乳动物细胞表达体系制备突变型GJB6重组蛋白，发现特定突变(如R75W)干扰线粒体膜电位，提出GJB6异常可能通过能量代谢途径影响皮肤及听力疾病的发展。
注：以上文献为示例，实际引用时建议通过PubMed或专业数据库核对具体信息。若需扩展，可搜索关键词“GJB6 recombinant protein”或“connexin 30 expression”。
**Background of GJB6 Recombinant Protein**
GJB6. encoding connexin 30 (Cx30), is a member of the connexin protein family, which forms gap junctions—specialized intercellular channels facilitating direct communication between adjacent cells. These channels enable the exchange of ions, metabolites, and signaling molecules, critical for maintaining tissue homeostasis and coordinating cellular activities. Cx30 is predominantly expressed in epithelial tissues, the inner ear, and the skin, where it plays roles in epidermal differentiation, cochlear function, and wound healing.
Mutations in *GJB6* are linked to human disorders, including hidrotic ectodermal dysplasia (Clouston syndrome) and nonsyndromic hearing loss. In the inner ear, Cx30 contributes to potassium ion recycling essential for auditory signal transduction. In the skin, it regulates keratinocyte differentiation and barrier formation. Dysfunctional Cx30 disrupts these processes, leading to pathological manifestations.
Recombinant GJB6 proteins are engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, disease mechanisms, and therapeutic potential. Purified recombinant Cx30 is utilized in *in vitro* assays to analyze channel permeability, oligomerization, and interactions with other connexins. It also serves as an antigen for antibody development or a tool in drug screening to identify modulators of gap junction activity.
Research on GJB6 recombinant protein advances understanding of connexin-related pathologies and supports the development of targeted therapies, such as small molecules to restore channel function or gene-editing approaches to correct mutations. Its applications extend to modeling tissue-specific gap junction networks and exploring their roles in development, cancer, and sensory organ dysfunction.
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