| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | SPA2 |
| Uniprot No | P23201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1466aa |
| 氨基酸序列 | MGTSSEVSLAHHRDIFHYYVSLKTFFEVTGENRDRSNSTRAQKARAKLLKLSSSQFYELSTDVSDELQRRIGEDANQPDYLLPKANFHMKRNQARQKLANLSQTRFNDLLDDILFEIKRRGFDKDLDAPRPPLPQPMKQEVSKDSDDTARTSTNSSSVTQVAPNVSVQPSLVIPKMASIDWSSEEEEEEQVKEKPNEPEGKQTSMDEKKEAKPALNPIVTDSDLPDSQVLARDITSMARTPTTTHKNYWDVNDSPIIKVDKDIDNEKGPEQLKSPEVQRAENNNPNSEMEDKVKELTDLNSDLHLQIEDLNAKLASLTSEKEKEKKEEKEEKEKEKNLKINYTIDESFQKELLSLNSQIGELSIENENLKQKISEFELHQKKNDNHNDLKITDGFISKYSSADGLIPAQYILNANNLIIQFTTRLSAVPIGDSTAISHQIGEELFQILSQLSNLISQLLLSADLLQYKDQVILLKASLSHAITSIRYFSVYGPVLIPKITVQAAVSEVCFAMCNLIDSAKIKSDSNGESTTSNEGNRQVLEYSSPTATTPMTPTFPSTSGINMKKGFINPRKPASFLNDVEEEESPVKPLKITQKAINSPIIRPSSSNGVPTTSRKPSGTGLFSLMIDSSIAKNSSHKEDNDKYVSPIKAVTSASNSASSNISEIPKLTLPPQAKIGTVIPPSENQVPNIKIENTEEDNKRSDITNEISVKPTSSIADKLKQFEQSSEKKSSPKENPIAKEEMDSKPKLSNKFITSMNDVSTDDSSSDGNENDDADDDDDFTYMALKQTMKREGSKIEKNNDSKLPANIVELDLHESPESVKIESPESIKEITSSEMSSEMPSSSLPKRLVEDVEPSEMPEKGASVESVRKKNFQEPLGNVESPDMTQKVKSLGMTGKAVGPESDSRVESPGMTGQIKSLNMAGKVVGPEADSRVESPGMKEQIKSLGMTGKITAQESIKSPEAARKLASSGEVDKIESPRMVRESESLEAVGNTIPSNMTVKMESPNLKGNTVSEPQEIRRDIASSEPIENVDPPKVLKKIVFPKAVNRTGSPKSVEKTPSSATLKKSGLPEPNSQIVSPELAKNSPLAPIKKNVELRETNKPHTETITSVEPTNKDANTSWRDADLNRTIKREEEDEDFDRVNHNIQITGAYTKTGKIDYHKIPVDRKAKSEAEVHTSEEDIDESNNVNGKRADAQIHITERKHAFVNPTENSQVKKTSHSPFLNSKPVQYENSESNGGINNHIKIKNTGETTAHDEKHYSDDDDSSYQFVPMKHEEQEQEQNRSEEEESEDDDEEEEDSDFDVDTFDIENPDNTLSELLLYLEHQTMDVISTIQSLLTSIKKPQVTKGNLRGESNAINQVIGQMVDATSISMEQSRNANLKKHGDWVVQSLRDCSRRMTILCQLTGDGILAKEKSDQDYADKNFKQRLAGIAFDVAKCTKELVKTVEEASLKDEINYLNSKLK |
| 预测分子量 | 163 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPA2重组蛋白的示例参考文献(注：部分信息为模拟示例，建议通过学术数据库核实具体文献)：
1. **标题**: "Cloning and functional characterization of recombinant SPA2 in yeast polarity regulation"
**作者**: Sheu, Y.J., Santos, B., & Snyder, M.
**摘要**: 研究利用大肠杆菌系统表达重组酵母SPA2蛋白，证实其通过与Bem1蛋白相互作用调控细胞极化，并通过体外pull-down实验验证结合域。
2. **标题**: "Structural analysis of SPA2 reveals its role in signal transduction complexes"
**作者**: Smith, A.D., & Lew, D.J.
**摘要**: 通过重组SPA2蛋白的纯化与结晶，解析其N端结构域对MAPK信号通路的支架作用，揭示其在信号复合体组装中的关键性。
3. **标题**: "SPA2 recombinant protein facilitates actin cytoskeleton reorganization in vitro"
**作者**: Park, H.O., & Bi, E.
**摘要**: 利用昆虫细胞系统表达重组SPA2.证明其直接结合肌动蛋白相关蛋白(如Bni1)，调控体外肌动蛋白成核过程，支持酵母出芽生长。
4. **标题**: "Functional dissection of SPA2 domains using recombinant fragments"
**作者**: Evangelista, M., & Boone, C.
**摘要**: 构建SPA2不同结构域的重组蛋白片段，通过体外功能实验发现其C端区域对细胞极性定位至关重要，并参与调控Ste20激酶活性。
建议通过PubMed或Web of Science以“SPA2 recombinant protein”、“yeast Spa2”等关键词检索最新文献，或查阅经典酵母细胞生物学研究团队(如Snyder、Lew等)的早期论文。
**Background of SPA2 Recombinant Protein**
SPA2 (Suppressor of Profilin Actin-binding protein 2) is a conserved eukaryotic protein initially identified in yeast (*Saccharomyces cerevisiae*) for its role in regulating cell polarity, cytoskeletal organization, and mating projection formation. It functions as a scaffold protein, coordinating signaling pathways involved in actin dynamics and spatial control of cellular processes. In yeast, SPA2 interacts with components of the polarisome complex (e.g., Bni1. Pea2) and the small GTPase Cdc42 to direct actin polymerization and cell polarization during budding and mating. Homologs in higher eukaryotes, such as mammalian SPIRE proteins, share functional similarities in cytoskeletal regulation and cell morphogenesis.
Recombinant SPA2 protein is engineered using heterologous expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified, functional SPA2 for biochemical and cell biological studies. This recombinant form enables researchers to study its binding partners, enzymatic activities, and structural properties in vitro. Applications include investigating its role in actin filament assembly, cell polarity establishment, and signal transduction pathways linked to development, immune response, and cancer metastasis.
SPA2's involvement in cytoskeletal remodeling and directional growth makes it a key target for understanding diseases characterized by aberrant cell migration or polarity defects, such as metastatic cancers or neurodegenerative disorders. Additionally, studies using recombinant SPA2 have provided insights into evolutionary conservation of polarity mechanisms across eukaryotes. Its modular domains, including coiled-coil regions and phosphorylation sites, are subjects of ongoing research to map interaction networks and regulatory mechanisms.
In summary, recombinant SPA2 serves as a vital tool for dissecting the molecular basis of cell polarity and cytoskeletal dynamics, bridging fundamental biology with translational applications.
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