| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSH |
| Uniprot No | Q47692 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1377aa |
| 氨基酸序列 | MNRIYSLRYSAVARGFIAVSEFARKCVHKSVRRLCFPVLLLIPVLFSAGSLAGTVNNELGYQLFRDFAENKGMFRPGATNIAIYNKQGEFVGTLDKAAMPDFSAVDSEIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVDRNNAPSLDFHAPRLDKLVTEVAPTAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTQMGGAYSWLTGGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLVGVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEGGALEWSFNSSTGAGALTQGTTTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSNGSTWTGAGIVVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVLNQQADNKGQVQAFSSVNIASGRPTVVLTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQLKAADYGAVLANNVDKRATITLDYALRADKVALNGWSESGKGTAGNLYKYNNPYTNTTDYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNTAGYLFHGQLKGNLNVDNRLPEGVTGALVMDGAADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHSVLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTIQADNSSVTLGDSRVFIDKNDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVNISSDSAVLGNSTLTSTALNLNKGANALASQSFVSDGPVNISDAALSLNSRPDEVSHTLLPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQMLYSLFNGYRNIWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTTLTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSNKDTLDIPLVSAPEATADNLFRASTRVVGFSDVTPILSVRKEDGKKEWVLDGYQVARNDGQGKAAATFMHISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADRKHELGSMDLFTGVMATYTDTDASADLYSGKTKSWGGGFYASGLFRSGAYFDVIAKYIHNENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNWNDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKDAAGEHQINGRKDSRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF |
| 预测分子量 | 148,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TSH(促甲状腺激素)重组蛋白的代表性文献摘要(文献标题、作者及内容均为虚构示例，仅用于格式演示)：
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1. **文献名称**: *Recombinant human TSH: Production and clinical application in thyroid cancer monitoring*
**作者**: Smith A, et al.
**摘要**: 研究利用CHO细胞表达系统高效生产重组人TSH(rhTSH)，验证其在分化型甲状腺癌患者术后随访中替代甲状腺激素撤退的诊断效果，证明rhTSH可减少患者低甲状腺素血症相关不适。
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2. **文献名称**: *Optimization of TSHβ subunit folding for enhanced recombinant protein stability*
**作者**: Chen L, Wang Y.
**摘要**: 通过定点突变和分子伴侣共表达技术，优化TSHβ亚基的折叠效率，解决了重组TSH蛋白易聚集的问题，显著提高其体外活性和储存稳定性。
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3. **文献名称**: *Comparative study of recombinant TSH vs. animal-derived TSH in diagnostic assays*
**作者**: Gonzalez R, et al.
**摘要**: 对比重组人TSH与牛源TSH在甲状腺功能检测试剂盒中的性能，证实重组蛋白具有更高的批次间一致性和特异性，推动体外诊断试剂的标准化进程。
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4. **文献名称**: *Crystal structure analysis of TSH receptor-binding domain*
**作者**: Tanaka K, et al.
**摘要**: 解析重组TSH蛋白与其受体的复合物晶体结构，揭示关键结合位点，为开发TSH类似物药物及甲状腺疾病治疗靶点提供结构生物学依据。
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(注：以上文献为模拟内容，实际研究需通过PubMed或Web of Science等数据库检索真实文献。)
**Background of Recombinant TSH Protein**
Thyroid-stimulating hormone (TSH), also known as thyrotropin, is a glycoprotein hormone produced by the anterior pituitary gland. It plays a central role in regulating thyroid function by binding to TSH receptors on thyroid follicular cells, stimulating the synthesis and secretion of thyroid hormones (T3 and T4) and promoting thyroid growth. TSH consists of two subunits: a common α-subunit shared with other glycoprotein hormones (e.g., LH, FSH) and a unique β-subunit that confers biological specificity.
Historically, TSH for clinical or research use was extracted from animal or human pituitary tissues, but this posed challenges, including limited supply, batch variability, and ethical concerns. The advent of recombinant DNA technology in the 1980s enabled the production of recombinant human TSH (rhTSH), eliminating reliance on biological sources. Recombinant TSH is synthesized by inserting genes encoding the α- and β-subunits into host cells (e.g., Chinese hamster ovary cells), which are cultured to secrete the glycosylated, bioactive heterodimer.
Recombinant TSH has revolutionized thyroid disease management. Clinically, it is used to stimulate thyroid tissue in diagnostic procedures (e.g., monitoring thyroid cancer recurrence) or therapeutic settings (e.g., enhancing radioiodine uptake in thyroid ablation), avoiding the hypothyroid state required with traditional hormone withdrawal. In research, it serves as a standardized tool for studying TSH receptor signaling, autoimmune thyroid disorders, and drug development. Its high purity, consistency, and reduced immunogenicity compared to animal-derived TSH have made it a cornerstone in endocrinology and biotechnology. Commercial versions, such as thyrotropin alfa (Thyrogen®), exemplify its therapeutic impact.
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