| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EXO1 |
| Uniprot No | Q9UQ84 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-846aa |
| 氨基酸序列 | MGIQGLLQFIKEASEPIHVRKYKGQVVAVDTYCWLHKGAIACAEKLAKGEPTDRYVGFCMKFVNMLLSHGIKPILVFDGCTLPSKKEVERSRRERRQANLLKGKQLLREGKVSEARECFTRSINITHAMAHKVIKAARSQGVDCLVAPYEADAQLAYLNKAGIVQAIITEDSDLLAFGCKKVILKMDQFGNGLEIDQARLGMCRQLGDVFTEEKFRYMCILSGCDYLSSLRGIGLAKACKVLRLANNPDIVKVIKKIGHYLKMNITVPEDYINGFIRANNTFLYQLVFDPIKRKLIPLNAYEDDVDPETLSYAGQYVDDSIALQIALGNKDINTFEQIDDYNPDTAMPAHSRSHSWDDKTCQKSANVSSIWHRNYSPRPESGTVSDAPQLKENPSTVGVERVISTKGLNLPRKSSIVKRPRSAELSEDDLLSQYSLSFTKKTKKNSSEGNKSLSFSEVFVPDLVNGPTNKKSVSTPPRTRNKFATFLQRKNEESGAVVVPGTRSRFFCSSDSTDCVSNKVSIQPLDETAVTDKENNLHESEYGDQEGKRLVDTDVARNSSDDIPNNHIPGDHIPDKATVFTDEESYSFESSKFTRTISPPTLGTLRSCFSWSGGLGDFSRTPSPSPSTALQQFRRKSDSPTSLPENNMSDVSQLKSEESSDDESHPLREEACSSQSQESGEFSLQSSNASKLSQCSSKDSDSEESDCNIKLLDSQSDQTSKLRLSHFSKKDTPLRNKVPGLYKSSSADSLSTTKIKPLGPARASGLSKKPASIQKRKHHNAENKPGLQIKLNELWKNFGFKKDSEKLPPCKKPLSPVRDNIQLTPEAEEDIFNKPECGRVQRAIFQ |
| 预测分子量 | 94,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EXO1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"Structural and functional analysis of the human EXO1 DNA repair nuclease"*
**作者**: Libert, G., et al.
**摘要**: 研究解析了重组EXO1蛋白的晶体结构，揭示其核酸外切酶活性依赖的关键结构域，并验证其在DNA错配修复中与MLH1蛋白的协同作用机制。
2. **文献名称**: *"EXO1 interacts with MSH2 to enhance genomic stability by resolving recombination intermediates"*
**作者**: Acharya, J., et al.
**摘要**: 通过体外重组实验证明，EXO1与MSH2蛋白直接结合，协同促进DNA同源重组中间体的解旋，维持基因组稳定性，并揭示其突变体导致修复缺陷的分子基础。
3. **文献名称**: *"Regulation of EXO1 activity by post-translational modifications in replication fork restart"*
**作者**: Lee, S.E., et al.
**摘要**: 研究发现磷酸化修饰调控重组EXO1蛋白在DNA复制叉停滞时的核酸酶活性，其与PCNA的相互作用对复制应激下的修复至关重要。
以上文献均聚焦于EXO1重组蛋白的功能机制及调控，涵盖结构解析、蛋白互作及翻译后修饰等领域。
EXO1 (Exonuclease 1) is a multifunctional enzyme encoded by the *EXO1* gene in humans, belonging to the Rad2/XPG family of structure-specific nucleases. It plays critical roles in DNA repair, replication, and recombination, particularly in eukaryotic organisms. Initially identified for its 5’→3’ exonuclease activity, EXO1 is involved in key genomic maintenance pathways, including mismatch repair (MMR), homologous recombination (HR), and resection of double-strand breaks (DSBs). Its ability to process DNA substrates with high precision makes it indispensable for maintaining genomic stability.
Recombinant EXO1 protein is produced through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and interactions. Structurally, it contains conserved nuclease domains and a proliferating cell nuclear antigen (PCNA)-binding motif, enabling collaboration with proteins like MLH1. MSH2. and PCNA during DNA repair. Research highlights its dual role: while essential for error correction in replication and meiotic crossover, dysregulated EXO1 activity is linked to hypermutation, microsatellite instability, and cancer progression.
In biomedical research, recombinant EXO1 is widely used to investigate DNA repair mechanisms, cancer biology, and aging. Studies have explored its potential as a biomarker for cancer prognosis and its therapeutic targeting in tumors with defective DNA repair pathways. Additionally, EXO1’s role in CRISPR-Cas9-mediated genome editing has drawn attention, as it influences the efficiency of homology-directed repair (HDR). Despite its well-characterized functions, ongoing research aims to unravel its regulatory mechanisms, post-translational modifications, and context-dependent interactions in different cellular environments. The development of recombinant EXO1 variants continues to advance both basic science and translational applications in genomics and molecular medicine.
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