| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPGS |
| Uniprot No | Q05932 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 氨基酸序列 | MSRARSHLRAALFLAAASARGITTQVAARRGLSAWPVPQEPSMEYQDAVRMLNTLQTNAGYLEQVKRQRGDPQTQLEAMELYLARSGLQVEDLDRLNIIHVTGTKGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRLYHRLEETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSSLGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQISCPLYLCPMLEALEEGGPPLTLGLEGEHQRSNAALALQLAHCWLQRQDRHGAGEPKASRPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVLRRGPLTWYLDGAHTASSAQACVRWFRQALQGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSAPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ |
| 预测分子量 | 64,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FPGS(叶酰聚谷氨酸合成酶)重组蛋白研究的代表性文献概览：
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1. **文献名称**: *Cloning and Expression of Human Folylpolyglutamate Synthetase*
**作者**: Shane B, et al.
**摘要**: 该研究首次报道了人源FPGS基因的克隆及在大肠杆菌中的重组表达，系统分析了重组酶的动力学特性，揭示了其底物特异性及在叶酸代谢中的关键作用，为后续药物靶点研究奠定基础。
2. **文献名称**: *Crystal Structure of Folylpolyglutamate Synthetase from Escherichia coli*
**作者**: Sun S, et al.
**摘要**: 通过X射线晶体学解析了大肠杆菌FPGS的三维结构，阐明了其催化口袋的构象及与ATP/叶酸底物的结合机制，为设计靶向抑制剂提供结构生物学依据。
3. **文献名称**: *Engineering FPGS Stability for Enhanced Antifolate Drug Efficacy in Cancer Cells*
**作者**: Li H, et al.
**摘要**: 通过定点突变优化重组FPGS的热稳定性，证明其在肿瘤细胞中过表达可显著增强甲氨蝶呤等抗叶酸药物的细胞内滞留及抗癌活性，为化疗增敏策略提供新思路。
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以上文献涵盖FPGS的基因克隆、结构解析及药物应用研究，如需具体年份或期刊信息，可进一步补充关键词进行精准检索。
**Background of FPGS Recombinant Protein**
Folylpolyglutamate synthase (FPGS) is a critical enzyme in folate metabolism, responsible for catalyzing the polyglutamylation of folates and antifolate drugs. This process involves the sequential addition of glutamate residues to folate molecules, converting them into polyglutamated forms that exhibit enhanced cellular retention and biological activity. FPGS-mediated polyglutamation is essential for maintaining intracellular folate pools, which are required for nucleotide synthesis, amino acid metabolism, and methylation reactions. Dysregulation of FPGS activity has been linked to diseases such as cancer, where altered folate metabolism supports rapid cell proliferation, and neurodevelopmental disorders due to impaired folate-dependent processes.
Recombinant FPGS proteins are engineered using recombinant DNA technology, enabling large-scale production in heterologous expression systems like *E. coli*, yeast, or mammalian cells. These proteins retain the enzymatic activity of native FPGS and serve as valuable tools for studying folate metabolism mechanisms, drug resistance in chemotherapy, and the development of antifolate therapeutics. For instance, antifolates like methotrexate rely on FPGS-mediated polyglutamation for prolonged intracellular retention and efficacy; thus, recombinant FPGS aids in understanding resistance mechanisms in cancer cells.
Additionally, FPGS recombinant proteins are utilized in structural biology to elucidate enzyme-substrate interactions and catalytic mechanisms through X-ray crystallography or cryo-EM. This structural insight supports rational drug design to optimize antifolate agents or modulate FPGS activity in disease contexts. Beyond oncology, FPGS research extends to agriculture and microbiology, where folate metabolism influences pathogen viability or crop resilience.
Overall, FPGS recombinant proteins bridge fundamental biochemical research with translational applications, offering insights into cellular metabolism and therapeutic innovation. Their versatility underscores their importance in both biomedical and biotechnological fields.
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