| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATK |
| Uniprot No | P42679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MAGRGSLVSWRAFHGCDSAEELPRVSPRFLRAWHPPPVSARMPTRRWAPG TQCITKCEHTRPKPGELAFRKGDVVTILEACENKSWYRVKHHTSGQEGLL AAGALREREALSADPKLSLMPWFHGKISGQEAVQQLQPPEDGLFLVRESA RHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDEAVFFCNLMDMVEHYSKDK GAICTKLVRPKRKHGTKSAEEELARAGWLLNLQHLTLGAQIGEGEFGAVL QGEYLGQKVAVKNIKCDVTAQAFLDETAVMTKMQHENLVRLLGVILHQGL YIVMEHVSKGNLVNFLRTRGRALVNTAQLLQFSLHVAEGMEYLESKKLVH RDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTAPEALKHGK FTSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVSEAVEKGYRMEPPEGCP GPVHVLMSSCWEAEPARRPPFRKLAEKLARELRSAGAPASVSGQDADGST SPRSQEP |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MATK(假设为某类激酶或特定蛋白)重组蛋白研究的参考文献示例(部分信息为虚构，仅供参考，建议通过学术数据库查询真实文献)：
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1. **文献名称**：Cloning and Functional Characterization of Recombinant MATK Protein in Cancer Signaling
**作者**：Smith J, et al.
**摘要**：本研究成功克隆并表达了人源MATK基因的重组蛋白，利用大肠杆菌表达系统进行纯化。实验表明，重组MATK通过调控下游磷酸化通路抑制肿瘤细胞增殖，为癌症靶向治疗提供潜在分子靶点。
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2. **文献名称**：Structural Analysis of MATK Kinase Domain and Its Role in Autoimmune Disorders
**作者**：Li Y, et al.
**摘要**：通过X射线晶体学解析了重组MATK激酶结构域的三维结构，揭示了其ATP结合位点的关键残基。功能实验证实，MATK异常活化与类风湿性关节炎中T细胞过度激活相关，为开发小分子抑制剂奠定基础。
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3. **文献名称**：High-Yield Production of Recombinant MATK in Insect Cells and Its Application in Drug Screening
**作者**：Garcia R, et al.
**摘要**：采用杆状病毒-昆虫细胞表达系统实现MATK重组蛋白的高效表达，纯度达95%以上。基于该蛋白建立高通量筛选平台，成功鉴定出两种新型MATK抑制剂，具有治疗神经退行性疾病的潜力。
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4. **文献名称**：MATK Recombinant Protein Modulates Neuronal Differentiation via MAPK Pathway
**作者**：Chen L, et al.
**摘要**：研究证明，重组MATK蛋白通过激活MAPK/ERK信号通路促进神经干细胞分化为神经元。基因敲减实验进一步验证了MATK在神经发育中的关键作用，为再生医学研究提供新思路。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实文献(关键词：MATK recombinant protein, MATK kinase, MATK gene expression)。
MATK (Megakaryocyte-Associated Tyrosine Kinase), also known as CHK (Csk-Homologous Kinase), is a non-receptor tyrosine kinase belonging to the CSK family. It plays a regulatory role in cellular signaling by phosphorylating and inhibiting Src-family kinases (SFKs), which are critical for processes like cell proliferation, differentiation, and immune responses. Discovered in the mid-1990s, MATK is predominantly expressed in hematopoietic cells, particularly megakaryocytes, neurons, and epithelial cells, highlighting its tissue-specific functions.
Recombinant MATK protein is produced using genetic engineering techniques, typically through expression in bacterial (e.g., *E. coli*) or mammalian systems. This allows large-scale production of purified, bioactive MATK for research and therapeutic applications. The recombinant form retains the kinase’s functional domains, including the SH3 and SH2 regions that mediate protein interactions and substrate recognition, as well as the catalytic kinase domain.
Studies using recombinant MATK have advanced understanding of its dual roles: suppressing SFK activity to maintain cellular homeostasis and modulating signaling pathways in diseases like cancer, inflammation, and neurological disorders. For instance, MATK dysregulation has been linked to leukemia and solid tumors, making it a potential target for kinase inhibitors. Additionally, recombinant MATK serves as a tool for high-throughput drug screening, structural studies, and antibody development.
Its therapeutic potential is being explored in immune modulation and targeted cancer therapies. However, challenges remain in optimizing expression systems for proper post-translational modifications and minimizing off-target effects in clinical applications. Ongoing research aims to unravel MATK’s precise mechanisms and expand its utility in precision medicine.
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