| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NFIA |
| Uniprot No | Q12857 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-509aa |
| 氨基酸序列 | MYSPLCLTQD EFHPFIEALL PHVRAFAYTW FNLQARKRKY FKKHEKRMSK EEERAVKDEL LSEKPEVKQK WASRLLAKLR KDIRPEYRED FVLTVTGKKP PCCVLSNPDQ KGKMRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERL VKSPQCSNPG LCVQPHHIGV SVKELDLYLA YFVHAADSSQ SESPSQPSDA DIKDQPENGH LGFQDSFVTS GVFSVTELVR VSQTPIAAGT GPNFSLSDLE SSSYYSMSPG AMRRSLPSTS STSSTKRLKS VEDEMDSPGE EPFYTGQGRS PGSGSQSSGW HEVEPGMPSP TTLKKSEKSG FSSPSPSQTS SLGTAFTQHH RPVITGPRAS PHATPSTLHF PTSPIIQQPG PYFSHPAIRY HPQETLKEFV QLVCPDAGQQ AGQVGFLNPN GSSQGKVHNP FLPTPMLPPP PPPPMARPVP LPVPDTKPPT TSTEGGAASP TSPTYSTPST SPANRFVSVG PRDPSFVNIP QQTQSWYLG |
| 预测分子量 | 55,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NFIA重组蛋白的3篇参考文献示例(内容基于假设性研究，实际文献需通过数据库验证)：
1. **"Structural analysis of recombinant NFIA protein in neural development"**
*作者：Smith J, et al.*
摘要：研究通过大肠杆菌表达系统纯化NFIA重组蛋白，解析其DNA结合结构域的晶体结构，揭示了NFIA在调控神经干细胞分化中的分子机制。
2. **"Functional characterization of NFIA recombinant protein in chromatin remodeling"**
*作者：Lee H, et al.*
摘要：利用哺乳动物细胞表达系统获得功能性NFIA重组蛋白，证明其通过与染色质修饰复合物相互作用，参与胚胎期肺上皮细胞的分化调控。
3. **"NFIA recombinant protein enhances direct reprogramming of fibroblasts to astrocytes"**
*作者：Chen R, et al.*
摘要：报道了NFIA重组蛋白与其它转录因子协同作用，显著提高体细胞向星形胶质细胞转分化的效率，为神经再生研究提供工具。
*注：以上为模拟摘要，实际文献建议通过PubMed/Google Scholar以“NFIA recombinant protein”为关键词检索获取。*
**Background of NFIA Recombinant Protein**
The Nuclear Factor I (NFI) family of transcription factors, including NFIA, NFIB, NFIC, and NFIX, plays critical roles in regulating gene expression during development and tissue-specific processes. NFIA (Nuclear Factor I A) is essential for organogenesis, particularly in the brain, spinal cord, and kidneys, where it governs cell differentiation, proliferation, and maintenance of stem/progenitor cell populations. It binds to specific DNA sequences via a conserved N-terminal DNA-binding domain and regulates transcription by forming homodimers or heterodimers with other NFI members.
NFIA recombinant protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional NFIA for experimental applications. This recombinant form retains the ability to interact with DNA and other proteins, enabling researchers to study its role in gene regulation, chromatin remodeling, and signaling pathways. Its production often includes affinity tags (e.g., His or GST tags) for simplified purification and detection.
Studies utilizing NFIA recombinant protein have highlighted its involvement in neurodevelopment, gliogenesis, and renal tubule formation. Dysregulation of NFIA is linked to developmental disorders, such as craniofacial abnormalities, and diseases like glioblastoma. Researchers also employ this protein to investigate epigenetic mechanisms, including DNA methylation and histone modification, and to model diseases *in vitro* or screen potential therapeutics targeting NFIA-associated pathways. Overall, NFIA recombinant protein serves as a vital tool for dissecting molecular mechanisms in development and disease.
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