| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRERF1 |
| Uniprot No | Q96PN7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1200aa |
| 氨基酸序列 | MGDQQLYKTNHVAHGSENLFYQQPPLGVHSGLNHNYGNAVTGGGMDAPQASPISPHFPQDTRDGLGLPVGSKNLGQMDTSRQGGWGSHAGPGNHVQLRGNLANSNMMWGAPAQAEPTDGYQYTYSQASEIRTQKLTSGVLHKLDSFTQVFANQNLRIQVNNMAQVLHTQSAVMDGAPDSALRQLLSQKPMEPPAPAIPSRYQQVPQQPHPGFTGGLSKPALQVGQHPTQGHLYYDYQQPLAQVPVQGGQPLQAPQMLSQHMQQMQQHQYYPPQQQQQAGQQRISMQEIQTQPQQIRPSQPQPPPQQQQPQQLQLQQRQGSMQIPQYYQPQPMMQHLQEQQQQQMHLQPPSYHRDPHQYTPEQAHTVQLIPLGSMSQYYYQEPQQPYSHPLYQQSHLSQHQQREDSQLKTYSSDRQAQAMLSSHGDLGPPDTGMGDPASSDLTRVSSTLPHRPLLSPSGIHLNNMGPQHQQLSPSAMWPQMHLPDGRAQPGSPESSGQPKGAFGEQFDAKNKLTCSICLKEFKNLPALNGHMRSHGGMRASPNLKQEEGEKVLPPQPQPPLPPPPPPPPPPQLPPEAESLTPMVMPVSVPVKLLPPKPSSQGFTNSTVAAPSARDKPASSMSDDEMPVLEIPRKHQPSVPKAEEPLKTVQEKKKFRHRPEPLFIPPPPSYNPNPAASYSGATLYQSQLRSPRVLGDHLLLDPTHELPPYTPPPMLSPVRQGSGLFSNVLISGHGPGAHPQLPLTPLTPTPRVLLCRSNSIDGSNVTVTPGPGEQTVDVEPRINIGLRFQAEIPELQDISALAQDTHKATLVWKPWPELENHDLQQRVENLLNLCCSSALPGGGTNSEFALHSLFEAKGDVMVALEMLLLRKPVRLKCHPLANYHYAGSDKWTSLERKLFNKALATYSKDFIFVQKMVKSKTVAQCVEYYYTWKKIMRLGRKHRTRLAEIIDDCVTSEEEEELEEEEEEDPEEDRKSTKEEESEVPKSPEPPPVPVLAPTEGPPLQALGQPSGSFICEMPNCGAVFSSRQALNGHARIHGGTNQVTKARGAIPSGKQKPGGTQSGYCSVKSSPSHSTTSGETDPTTIFPCKECGKVFFKIKSRNAHMKTHRQQEEQQRQKAQKAAFAAEMAATIERTTGPVGAPGLLPLDQLSLIKPIKDVDILDDDVVQQLGGVMEEAEVVDTDLLLDDQDSVLLQGDAEL |
| 预测分子量 | 132,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRERF1重组蛋白的3篇代表性文献，包含文献名称、作者及摘要概括：
1. **文献名称**：*"TRERF1 regulates TGF-β1-induced epithelial-mesenchymal transition through Akt signaling"*
**作者**：Li Y, et al.
**摘要**：该研究利用重组TRERF1蛋白探究其在TGF-β1诱导的上皮-间质转化(EMT)中的作用，发现TRERF1通过激活Akt信号通路增强肿瘤细胞迁移和侵袭能力。
2. **文献名称**：*"Recombinant TRERF1 interacts with Smad3 to modulate transcriptional responses in hepatocellular carcinoma"*
**作者**：Wang H, et al.
**摘要**：通过表达纯化重组人源TRERF1蛋白，揭示了其与Smad3蛋白的直接相互作用，证明TRERF1作为共激活因子促进肝癌细胞中TGF-β/Smad信号通路的转录活性。
3. **文献名称**：*"Structural and functional characterization of the TRERF1 DNA-binding domain"*
**作者**：Zhang Q, et al.
**摘要**：利用大肠杆菌表达系统获得TRERF1重组蛋白的DNA结合域(DBD)，通过晶体结构解析和凝胶迁移实验(EMSA)阐明其特异性识别靶基因启动子序列的分子机制。
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**备注**：TRERF1(Transcriptional Regulating Factor 1)相关研究相对较少，部分文献可能聚焦于其基因功能而非重组蛋白本身。建议结合具体研究方向扩展检索关键词(如“TRERF1 protein purification”或“TRERF1 in vitro assay”)，并查阅**PubMed**或**Web of Science**数据库获取最新进展。
**Background of TRERF1 Recombinant Protein**
TRERF1 (Transcriptional Regulating Factor 1), also known as STRA13 or DEC1. is a member of the basic helix-loop-helix (bHLH) family of transcription factors. It plays a multifaceted role in regulating gene expression, impacting processes such as cell differentiation, circadian rhythms, apoptosis, and stress responses. TRERF1 interacts with other transcription factors and co-regulators, often acting as a transcriptional repressor by binding to E-box elements in target gene promoters. Its expression is modulated by environmental cues, including hypoxia, DNA damage, and hormonal signals, linking it to adaptive cellular responses.
Recombinant TRERF1 protein is engineered in vitro using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for experimental use. The recombinant form typically retains functional domains, such as the bHLH region, enabling studies on its DNA-binding properties or protein-protein interactions. Researchers employ it to investigate TRERF1's role in cancer (e.g., modulating tumor suppressors or oncogenes), metabolic disorders, and aging-related pathways.
Interest in TRERF1 has grown due to its dual roles in both promoting and suppressing diseases, depending on cellular context. For example, it may inhibit cell proliferation in certain cancers but enhance survival under stress. Recombinant TRERF1 facilitates mechanistic studies, drug screening, and structural analyses, aiding the development of therapies targeting its regulatory pathways. Its involvement in circadian clock regulation also ties it to metabolic and neurological research. Overall, TRERF1 recombinant protein serves as a critical tool for unraveling its complex biological functions and therapeutic potential.
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