| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | gABBR1 |
| Uniprot No | O15205 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MAPNASCLCVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGIRKGNLLFLACYCIGG |
| 预测分子量 | 34.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GABBR1重组蛋白的3篇参考文献概述(文献标题与作者为虚构示例，供参考)：
1. **标题**：Structural insights into GABBR1 activation through recombinant expression
**作者**：Smith A, et al.
**摘要**：研究通过重组表达系统获得人源GABBR1蛋白，解析其跨膜结构域构象变化，揭示了GABA结合后受体激活的分子机制。
2. **标题**：Functional characterization of recombinant GABBR1/GABBR2 heterodimers in neuronal signaling
**作者**：Chen L, et al.
**摘要**：利用重组共表达技术构建GABBR1/GABBR2异源二聚体，证实其介导细胞内cAMP信号抑制功能，并验证了特定激动剂对受体复合物的选择性调控。
3. **标题**：Development of a high-yield recombinant GABBR1 production system in mammalian cells
**作者**：Yamamoto K, et al.
**摘要**：优化哺乳动物细胞中GABBR1重组蛋白的表达与纯化策略，获得高纯度蛋白用于抗体开发及药物筛选，提升下游药理研究效率。
---
*注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索真实文献(关键词：GABBR1 recombinant, GABA-B receptor expression)。*
**Background of gABBR1 Recombinant Protein**
The GABAB receptor 1 (GABBR1 or GABABR1) is a critical subunit of the GABAB receptor, a class C G-protein-coupled receptor (GPCR) that mediates the slow and prolonged inhibitory effects of γ-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. GABAB receptors function as heterodimers, requiring both GABABR1 and GABABR2 subunits for proper trafficking, ligand binding, and downstream signaling. The GABABR1 subunit is primarily responsible for GABA binding, while GABABR2 facilitates coupling to intracellular G-proteins, activating secondary effectors like potassium channels or inhibiting adenylyl cyclase.
Recombinant gABBR1 protein is engineered in vitro using expression systems such as mammalian, insect, or bacterial cells to produce purified, functional protein for research applications. Its production enables detailed studies on receptor structure, ligand interactions, and signaling mechanisms. Recombinant gABBR1 is pivotal in drug discovery, particularly for neurological disorders (e.g., epilepsy, anxiety, chronic pain), as GABAB receptors are therapeutic targets for muscle relaxants, anticonvulsants, and potential antidepressants.
Advances in recombinant technology have resolved challenges like receptor solubility and stability, allowing crystallographic studies and high-throughput screening of modulators. Mutagenesis studies using recombinant gABBR1 further elucidate binding sites and allosteric regulation. However, complexities in preserving native conformation and post-translational modifications during heterologous expression remain considerations. Overall, recombinant gABBR1 serves as a vital tool for advancing neuroscience research and developing targeted neuromodulatory therapies.
×
