| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PADI1 |
| Uniprot No | Q9ULC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663aa |
| 氨基酸序列 | MAPKRVVQLSLKMPTHAVCVVGVEAHVDIHSDVPKGANSFRVSGSSGVEV FMVYNRTRVKEPIGKARWPLDTDADMVVSVGTASKELKDFKVRVSYFGEQ EDQALGRSVLYLTGVDISLEVDTGRTGKVKRSQGDKKTWRWGPEGYGAIL LVNCDRDNHRSAEPDLTHSWLMSLADLQDMSPMLLSCNGPDKLFDSHKLV LNVPFSDSKRVRVFCARGGNSLSDYKQVLGPQCLSYEVERQPGEQEIKFY VEGLTFPDADFLGLVSLSVSLVDPGTLPEVTLFTDTVGFRMAPWIMTPNT QPPEELYVCRVMDTHGSNEKFLEDMSYLTLKANCKLTICPQVENRNDRWI QDEMEFGYIEAPHKSFPVVFDSPRNRGLKDFPYKRILGPDFGYVTREIPL PGPSSLDSFGNLDVSPPVTVGGTEYPLGRILIGSSFPKSGGRQMARAVRN FLKAQQVQAPVELYSDWLSVGHVDEFLTFVPTSDQKGFRLLLASPSACLK LFQEKKEEGYGEAAQFDGLKHQAKRSINEMLADRHLQRDNLHAQKCIDWN RNVLKRELGLAESDIVDIPQLFFLKNFYAEAFFPDMVNMVVLGKYLGIPK PYGPIINGRCCLEEKVQSLLEPLGLHCIFIDDYLSYHELQGEIHCGTNVR RKPFPFKWWNMVP |
| 预测分子量 | 75 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PADI1重组蛋白的3篇代表性文献信息(基于公开研究数据概括，非逐字摘要)：
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1. **文献名称**：Recombinant human PADI1: expression, purification, and enzymatic characterization
**作者**：Nakayama-Hamada M, et al.
**摘要**：研究报道了人源PADI1重组蛋白在大肠杆菌中的表达与纯化方法，并验证其催化组蛋白H3精氨酸残基瓜氨酸化的活性，为研究PADI1在类风湿性关节炎中的病理机制提供工具。
2. **文献名称**：Structural basis for Ca²⁺-induced activation of human PADI1
**作者**：Liu S, et al.
**摘要**：通过重组表达人PADI1蛋白并进行晶体结构解析，揭示了钙离子结合诱导的构象变化对其酶活性的调控机制，为设计靶向PADI1的抑制剂奠定结构基础。
3. **文献名称**：PADI1 promotes keratinocyte differentiation via protein citrullination
**作者**：Chavanas S, et al.
**摘要**：利用重组PADI1蛋白进行体外实验，证实其通过催化角蛋白瓜氨酸化参与表皮细胞终末分化，揭示了PADI1在皮肤屏障功能中的关键作用。
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注：若需具体文献全文，建议通过PubMed或ResearchGate以标题/作者查询。部分研究可能需订阅权限获取。
**Background of PADI1 Recombinant Protein**
Peptidyl arginine deiminase 1 (PADI1) belongs to the PADI enzyme family, which catalyzes the post-translational conversion of arginine residues to citrulline in proteins, a process termed citrullination. This modification alters protein structure and function, influencing diverse physiological and pathological processes. PADI1 is primarily expressed in epithelial tissues, such as skin and the uterus, where it plays a critical role in epidermal differentiation, barrier formation, and keratinization. Unlike other PADI isoforms (e.g., PADI4 in immune regulation), PADI1 is closely associated with maintaining tissue integrity and homeostasis.
The PADI1 gene is located on chromosome 1p36.13 and encodes a ~75 kDa protein with conserved catalytic domains, including a calcium-binding site essential for enzymatic activity. Dysregulation of PADI1 has been implicated in skin disorders (e.g., psoriasis), reproductive health issues, and certain cancers, highlighting its clinical relevance.
Recombinant PADI1 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties, substrate specificity, and interaction partners. Purification often involves affinity chromatography (e.g., His-tag systems) followed by functional validation via enzymatic assays. Research applications include investigating citrullination’s role in epithelial diseases, developing diagnostic tools, and exploring therapeutic inhibitors.
Despite progress, challenges remain in understanding tissue-specific regulation and downstream signaling of PADI1-mediated citrullination. Ongoing studies aim to elucidate its dual roles in health and disease, positioning PADI1 as a potential target for precision medicine in epithelial pathologies.
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