| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACYP1 |
| Uniprot No | P07311 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1- 99aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAEGNTLISVDYEIFGKVQGVFFRKHT QAEGKKLGLVGWVQNTDRGTVQGQLQGPISKVRHMQEWLETRGSPKSHID KANFNNEKVILKLDYSDFQIVK |
| 预测分子量 | 14 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的3篇关于ACYP1重组蛋白的参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**：*"Recombinant expression and functional characterization of human ACYP1 in Escherichia coli"*
**作者**：Chen et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人源ACYP1蛋白，通过酶活性实验证实其具有水解酰基磷酸酯的催化功能，并发现其活性受pH和金属离子调控。
2. **文献名称**：*"Structural analysis of ACYP1 reveals insights into substrate specificity"*
**作者**：Wang et al.
**摘要**：利用X射线晶体学解析了重组ACYP1的三维结构，揭示了其底物结合口袋的关键氨基酸残基，为设计靶向抑制剂提供了结构基础。
3. **文献名称**：*"ACYP1 overexpression exacerbates oxidative stress in neuronal cells: Implications for neurodegenerative diseases"*
**作者**：Kimura et al.
**摘要**：通过体外表达重组ACYP1蛋白，发现其过表达导致神经元活性氧(ROS)水平升高，提示ACYP1可能参与神经退行性病变的病理过程。
---
**备注**：以上内容为示例，实际文献建议通过PubMed、Web of Science或Google Scholar检索关键词“ACYP1 recombinant protein”或“Acylphosphatase 1 expression”获取。
ACYP1 (Acylphosphatase-1) is a small cytosolic enzyme belonging to the acylphosphatase family, which catalyzes the hydrolysis of acyl phosphate bonds in metabolites like carbamoyl phosphate and 1.3-bisphosphoglycerate. It is encoded by the ACYP1 gene located on human chromosome 14q23.3. Structurally, ACYP1 consists of ~98 amino acids, forming a compact α/β-fold with a conserved active site. While its precise physiological role remains under investigation, ACYP1 is hypothesized to regulate cellular energy metabolism, ion channel activity, and redox balance. It is ubiquitously expressed but shows higher levels in erythrocytes, muscle, and brain tissues.
Recombinant ACYP1 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling studies of its enzymatic properties and interactions. Purification methods often involve affinity tags (e.g., His-tag) and chromatography. Research highlights its potential involvement in neurodegenerative diseases, as ACYP1 overexpression has been linked to altered calcium signaling and reactive oxygen species (ROS) modulation in neuronal models. Additionally, ACYP1 may influence erythrocyte membrane stability by interacting with anion transporters. Its dysregulation has been observed in certain cancers, though mechanistic insights remain limited.
Recent studies utilize recombinant ACYP1 to explore its role in protein misfolding disorders, including Alzheimer’s disease, where it may interact with amyloid-β peptides. Structural analyses of recombinant ACYP1 also aid in designing inhibitors to probe its function. Despite progress, the enzyme’s full biological significance and therapeutic potential require further elucidation through in vivo models and omics approaches.
×
