| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPN21 |
| Uniprot No | Q16825 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1174aa |
| 氨基酸序列 | MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELREVTYFSLWYYNKQNQRRWVDLEKPLKKQLDKYALEPTVYFGVVFYVPSVSQLQQEITRYQYYLQLKKDILEGSIPCTLEQAIQLAGLAVQADFGDFDQYESQDFLQKFALFPVGWLQDEKVLEEATQKVALLHQKYRGLTAPDAEMLYMQEVERMDGYGEESYPAKDSQGSDISIGACLEGIFVKHKNGRHPVVFRWHDIANMSHNKSFFALELANKEETIQFQTEDMETAKYIWRLCVARHKFYRLNQCNLQTQTVTVNPIRRRSSSRMSLPKPQPYVMPPPPQLHYNGHYTEPYASSQDNLFVPNQNGYYCHSQTSLDRAQIDLNGRIRNGSVYSAHSTNSLNNPQPYLQPSPMSSNPSITGSDVMRPDYLPSHRHSAVIPPSYRPTPDYETVMKQLNRGLVHAERQSHSLRNLNIGSSYAYSRPAALVYSQPEIREHAQLPSPAAAHCPFSLSYSFHSPSPYPYPAERRPVVGAVSVPELTNAQLQAQDYPSPNIMRTQVYRPPPPYPPPRPANSTPDLSRHLYISSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLTAARHAQLHKRNSIEVAGLSHGLEGLRLKERTLSASAAEVAPRAVSVGSQPSVFTERTQREGPEEAEGLRYGHKKSLSDATMLIHSSEEEEDEDFEEESGARAPPARAREPRPGLAQDPPGCPRVLLAGPLHILEPKAHVPDAEKRMMDSSPVRTTAEAQRPWRDGLLMPSMSESDLTTSGRYRARRDSLKKRPVSDLLSGKKNIVEGLPPLGGMKKTRVDAKKIGPLKLAALNGLSLSRVPLPDEGKEVATRATNDERCKILEQRLEQGMVFTEYERILKKRLVDGECSTARLPENAERNRFQDVLPYDDVRVELVPTKENNTGYINASHIKVSVSGIEWDYIATQGPLQNTCQDFWQMVWEQGIAIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKMKHLLTGQERTVWHLQYTDWPEHGCPEDLKGFLSYLEEIQSVRRHTNSTSDPQSPNPPLLVHCSAGVGRTGVVILSEIMIACLEHNEVLDIPRVLDMLRQQRMMLVQTLCQYTFVYRVLIQFLKSSRLI |
| 预测分子量 | 133,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPN21重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*"PTPN21 regulates focal adhesion dynamics by targeting tyrosine-phosphorylated RACK1"*
**作者**：Barr AJ, et al.
**摘要**：该研究探讨了PTPN21作为非受体型酪氨酸磷酸酶的功能，发现其重组蛋白通过去磷酸化支架蛋白RACK1调控黏着斑动力学，影响细胞迁移和侵袭过程。
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2. **文献名称**：*"PTPN21 interacts with EGFR to promote cancer cell motility by modulating Rho GTPase signaling"*
**作者**：Huang Y, et al.
**摘要**：通过重组蛋白表达实验，揭示了PTPN21与表皮生长因子受体(EGFR)的相互作用，表明其通过调控Rho家族GTP酶活性促进肿瘤细胞迁移，为癌症转移机制提供新见解。
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3. **文献名称**：*"Structural and functional analysis of PTPN21 reveals a substrate recognition mechanism involving its SH3 domain"*
**作者**：Smith GP, et al.
**摘要**：该研究解析了重组PTPN21蛋白的SH3结构域与其底物的结合模式，阐明了其通过特异性识别磷酸酪氨酸位点参与细胞内信号转导的分子机制。
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**注**：以上文献信息为示例，实际引用需以具体论文内容为准。如需真实文献，建议通过PubMed或Google Scholar搜索关键词“PTPN21 recombinant protein”获取最新研究。
**Background of PTPN21 Recombinant Protein**
PTPN21 (Protein Tyrosine Phosphatase Non-Receptor Type 21) is a member of the protein tyrosine phosphatase (PTP) family, which regulates cellular signaling by dephosphorylating tyrosine residues on target proteins. This enzyme plays a critical role in modulating signal transduction pathways involved in cell adhesion, migration, and immune responses. Structurally, PTPN21 contains an N-terminal FERM (Four-point-one, Ezrin, Radixin, Moesin) domain, which facilitates protein-protein interactions and membrane localization, and a C-terminal catalytic PTP domain responsible for its enzymatic activity.
The recombinant PTPN21 protein is engineered through *in vitro* expression systems (e.g., mammalian, bacterial, or insect cells) to produce a purified, functional form of the enzyme. This recombinant tool often includes affinity tags (e.g., His or GST tags) for efficient purification and detection. Studies using PTPN21 recombinant protein have elucidated its interactions with signaling molecules like EGFR, Src kinases, and cytoskeletal regulators, highlighting its role in cancer progression, neuronal development, and immune regulation. Dysregulation of PTPN21 has been linked to diseases such as breast cancer, Alzheimer’s, and inflammatory disorders, making it a potential therapeutic target.
Research applications include enzymatic activity assays, substrate identification, and screening for inhibitors or activators. Its recombinant form is vital for deciphering molecular mechanisms and developing targeted therapies. Overall, PTPN21 recombinant protein serves as a key reagent in exploring tyrosine phosphatase biology and its implications in health and disease.
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