| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRU |
| Uniprot No | Q92729 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1446aa |
| 氨基酸序列 | MARAQALVLALTFQLCAPETETPAAGCTFEEASDPAVPCEYSQAQYDDFQWEQVRIHPGTRAPADLPHGSYLMVNTSQHAPGQRAHVIFQSLSENDTHCVQFSYFLYSRDGHSPGTLGVYVRVNGGPLGSAVWNMTGSHGRQWHQAELAVSTFWPNEYQVLFEALISPDRRGYMGLDDILLLSYPCAKAPHFSRLGDVEVNAGQNASFQCMAAGRAAEAERFLLQRQSGALVPAAGVRHISHRRFLATFPLAAVSRAEQDLYRCVSQAPRGAGVSNFAELIVKEPPTPIAPPQLLRAGPTYLIIQLNTNSIIGDGPIVRKEIEYRMARGPWAEVHAVSLQTYKLWHLDPDTEYEISVLLTRPGDGGTGRPGPPLISRTKCAEPMRAPKGLAFAEIQARQLTLQWEPLGYNVTRCHTYTVSLCYHYTLGSSHNQTIRECVKTEQGVSRYTIKNLLPYRNVHVRLVLTNPEGRKEGKEVTFQTDEDVPSGIAAESLTFTPLEDMIFLKWEEPQEPNGLITQYEISYQSIESSDPAVNVPGPRRTISKLRNETYHVFSNLHPGTTYLFSVRARTGKGFGQAALTEITTNISAPSFDYADMPSPLGESENTITVLLRPAQGRGAPISVYQVIVEEERARRLRREPGGQDCFPVPLTFEAALARGLVHYFGAELAASSLPEAMPFTVGDNQTYRGFWNPPLEPRKAYLIYFQAASHLKGETRLNCIRIARKAACKESKRPLEVSQRSEEMGLILGICAGGLAVLILLLGAIIVIIRKGRDHYAYSYYPKPVNMTKATVNYRQEKTHMMSAVDRSFTDQSTLQEDERLGLSFMDTHGYSTRGDQRSGGVTEASSLLGGSPRRPCGRKGSPYHTGQLHPAVRVADLLQHINQMKTAEGYGFKQEYESFFEGWDATKKKDKVKGSRQEPMPAYDRHRVKLHPMLGDPNADYINANYIDGYHRSNHFIATQGPKPEMVYDFWRMVWQEHCSSIVMITKLVEVGRVKCSRYWPEDSDTYGDIKIMLVKTETLAEYVVRTFALERRGYSARHEVRQFHFTAWPEHGVPYHATGLLAFIRRVKASTPPDAGPIVIHCSAGTGRTGCYIVLDVMLDMAECEGVVDIYNCVKTLCSRRVNMIQTEEQYIFIHDAILEACLCGETTIPVSEFKATYKEMIRIDPQSNSSQLREEFQTLNSVTPPLDVEECSIALLPRNRDKNRSMDVLPPDRCLPFLISTDGDSNNYINAALTDSYTRSAAFIVTLHPLQSTTPDFWRLVYDYGCTSIVMLNQLNQSNSAWPCLQYWPEPGRQQYGLMEVEFMSGTADEDLVARVFRVQNISRLQEGHLLVRHFQFLRWSAYRDTPDSKKAFLHLLAEVDKWQAESGDGRTIVHCLNGGGRSGTFCACATVLEMIRCHNLVDVFFAAKTLRNYKPNMVETMDQYHFCYDVALEYLEGLESR |
| 预测分子量 | 162,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRU重组蛋白的3篇参考文献及其简要摘要：
1. **"Expression and functional analysis of recombinant PTPRU phosphatase domain in Wnt/β-catenin signaling"**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过大肠杆菌系统表达了PTPRU的催化结构域重组蛋白，验证其磷酸酶活性，并发现其通过去磷酸化β-catenin抑制Wnt信号通路，揭示了PTPRU在肿瘤发生中的潜在调控机制。
2. **"Structural characterization of PTPRU extracellular domain and its role in cell adhesion"**
- **作者**: Lee J, et al.
- **摘要**: 文章利用哺乳动物细胞表达系统制备了PTPRU胞外段重组蛋白，解析其晶体结构，证明其与细胞表面粘附分子相互作用，调控上皮细胞极性及屏障功能。
3. **"Development of a PTPRU recombinant protein-based assay for phosphatase activity screening"**
- **作者**: Chen L, et al.
- **摘要**: 该研究通过昆虫细胞表达纯化全长PTPRU重组蛋白，建立高通量磷酸酶活性检测体系，用于筛选小分子抑制剂，为靶向PTPRU的癌症治疗提供工具。
注：以上文献为示例，实际引用时请核实具体论文信息及数据库收录情况。
**Background of PTPRU Recombinant Protein**
PTPRU (Protein Tyrosine Phosphatase Receptor Type U) is a member of the protein tyrosine phosphatase (PTP) family, which plays a critical role in regulating cellular signaling by dephosphorylating tyrosine residues on target proteins. This enzyme is integral to maintaining the balance of tyrosine phosphorylation, a key mechanism controlling cell adhesion, migration, proliferation, and differentiation. PTPRU, also known as PTPρ or PTP-LAR, is a transmembrane receptor-type phosphatase characterized by an extracellular fibronectin-type III domain, a single transmembrane region, and a cytoplasmic catalytic domain. Its structure suggests involvement in cell-cell and cell-matrix interactions, particularly in epithelial and endothelial tissues.
Dysregulation of PTPRU has been implicated in various pathologies, including cancer and developmental disorders. For instance, reduced PTPRU expression is linked to increased cell invasiveness in colorectal cancer, while aberrant signaling contributes to tumor progression and metastasis in other malignancies. Additionally, PTPRU interacts with signaling pathways such as Wnt/β-catenin and Hippo, highlighting its role in tissue homeostasis and disease.
Recombinant PTPRU protein is engineered using expression systems (e.g., bacterial, mammalian) to produce purified, functional enzyme for research and therapeutic applications. It enables detailed study of PTPRU’s enzymatic activity, substrate specificity, and regulatory mechanisms. Researchers utilize it to investigate its role in disease models, screen inhibitors for drug development, or restore normal phosphatase activity in pathological conditions. Challenges in producing active recombinant PTPRU include preserving its native conformation and catalytic efficiency, often addressed through optimized expression and purification strategies.
Overall, PTPRU recombinant protein serves as a vital tool for unraveling its biological functions and therapeutic potential in tyrosine phosphorylation-related diseases.
×
