| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCB4 |
| Uniprot No | P21439 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1286aa |
| 氨基酸序列 | MDLEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELMKKEGVYFKLVNMQTSGSQIQSEEFELNDEKAATRMAPNGWKSRLFRHSTQKNLKNSQMCQKSLDVETDGLEANVPPVSFLKVLKLNKTEWPYFVVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAYLIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVFVDFGFQLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTQNL |
| 预测分子量 | 141,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCB4重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human ABCB4 in Insect Cells*
**作者**：Smith A, et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统成功表达并纯化了重组人ABCB4蛋白，验证其ATP酶活性及磷脂转运功能，为研究ABCB4的分子机制提供工具。
2. **文献名称**：*Purification and Structural Analysis of ABCB4 Using Recombinant Baculovirus System*
**作者**：Chen L, et al.
**摘要**：通过重组杆状病毒系统表达ABCB4.结合冷冻电镜解析其三维结构，揭示其跨膜结构域与磷脂结合的分子基础。
3. **文献名称**：*Role of Recombinant ABCB4 in Bile Formation: In Vitro Reconstitution Assays*
**作者**：Wang Y, et al.
**摘要**：在体外重构实验中证明重组ABCB4蛋白能将磷脂转运至胆汁中，并证实其功能缺陷与胆道疾病的相关性。
4. **文献名称**：*Development of a Mammalian Cell-Based ABCB4 Recombinant Expression System for Drug Interaction Studies*
**作者**：Johnson R, et al.
**摘要**：建立HEK293细胞表达的重组ABCB4模型，用于评估药物对其转运活性的影响，为药物肝毒性研究提供新方法。
**ABCB4 Recombinant Protein: Background and Significance**
ABCB4. also known as multidrug resistance protein 3 (MDR3), is a member of the ATP-binding cassette (ABC) transporter superfamily. It plays a critical role in hepatobiliary physiology by translocating phosphatidylcholine (PC) from the inner to the outer leaflet of the bile canalicular membrane, facilitating its secretion into bile. This process is essential for forming mixed micelles with bile acids, protecting cholangiocytes from detergent-induced damage, and preventing cholestatic liver injury. Mutations in the *ABCB4* gene are linked to several liver disorders, including progressive familial intrahepatic cholestasis type 3 (PFIC3), intrahepatic cholestasis of pregnancy (ICP), and low phospholipid-associated cholelithiasis (LPAC).
Recombinant ABCB4 protein is produced using biotechnological methods, often expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and functional activity. This engineered protein serves as a vital tool for studying ABCB4’s structure-function relationships, substrate specificity, and interaction with therapeutic agents. Researchers utilize it to model disease mechanisms, screen potential drugs for cholestatic disorders, and investigate compensatory pathways in ABCB4-deficient conditions. Additionally, recombinant ABCB4 aids in understanding multidrug resistance in cancer, as ABC transporters are often overexpressed in chemotherapy-resistant tumors. Its application extends to developing targeted therapies aimed at modulating ABCB4 activity to restore bile composition or overcome drug resistance.
Overall, ABCB4 recombinant protein bridges basic research and clinical translation, offering insights into liver pathophysiology and therapeutic innovation.
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