| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCB6 |
| Uniprot No | Q9NP58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 598-818aa |
| 氨基酸序列 | MVTVGNYCEAEGPVGPAWMQDGLSPCFFFTLVPSTRMALGTLALVLALPCRRRERPAGADSLSWGAGPRISPYVLQLLLATLQAALPLAGLAGRVGTARGAPLPSYLLLASVLESLAGACGLWLLVVERSQARQRLAMGIWIKFRHSPGLLLLWTVAFAAENLALVSWNSPQWWWARADLGQQVQFSLWVLRYVVSGGLFVLGLWAPGLRPQSYTLQVHEEDQDVERSQVRSAAQQSTWRDFGRKLRLLSGYLWPRGSPALQLVVLICLGLMGLERALNVLVPIFYRNIVNLLTEKAPWNSLAWTVTSYVFLKFLQGGGTGSTGFVSNLRTFLWIRVQQFTSRRVELLIFSHLHELSLRWHLGRRTGEVLRIADRGTSSVTGLLSYLVFNVIPTLADIIIGIIYFSMFFNAWFGLIVFLCMSLYLTLTIVVTEWRTKFRRAMNTQENATRARAVDSLLNFETVKYYNAESYEVERYREAIIKYQGLEWKSSASLVLLNQTQNLVIGLGLLAGSLLCAYFVTEQKLQVGDYVLFGTYIIQLYMPLNWFGTYYRMIQTNFIDMENMFDLLKEETEVKDLPGAGPLRFQKGRIEFENVHFSYADGRETLQDVSFTVMPGQTLALVGPSGAGKSTILRLLFRFYDISSGCIRIDGQDISQVTQASLRSHIGVVPQDTVLFNDTIADNIRYGRVTAGNDEVEAAAQAAGIHDAIMAFPEGYRTQVGERGLKLSGGEKQRVAIARTILKAPGIILLDEATSALDTSNERAIQASLAKVCANRTTIVVAHRLSTVVNADQILVIKDGCIVERGRHEALLSRGGVYADMWQLQQGQEETSEDTKPQTMER |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCB6重组蛋白的3篇参考文献示例(虚构内容，仅作格式参考)：
1. **文献名称**：*Structural and functional analysis of recombinant human ABCB6 protein*
**作者**：Zhang Y, et al.
**摘要**：通过昆虫细胞表达系统成功获得ABCB6重组蛋白，解析其晶体结构，揭示其底物结合口袋特征，并验证其在胞内血红素转运中的关键作用。
2. **文献名称**：*Recombinant ABCB6 expression in yeast and its role in drug resistance*
**作者**：Smith J, et al.
**摘要**：利用毕赤酵母系统高效表达ABCB6重组蛋白，证明其过表达可增强细胞对化疗药物的耐受性，可能与肿瘤多药耐药性相关。
3. **文献名称**：*ABCB6 knockout and recombinant rescue models elucidate mitochondrial porphyrin transport*
**作者**：Lee H, et al.
**摘要**：构建ABCB6基因敲除细胞系，并通过重组蛋白回补实验证实ABCB6在线粒体卟啉代谢中的必需性，为遗传性皮肤病研究提供机制依据。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed。)
ABCB6. a member of the ATP-binding cassette (ABC) transporter superfamily, is a transmembrane protein involved in the transport of various substrates across biological membranes. It is ubiquitously expressed in humans, with prominent localization to the mitochondria and lysosomes. Functionally, ABCB6 plays critical roles in heme biosynthesis, iron homeostasis, and cellular detoxification. It facilitates the transport of porphyrins and their intermediates, particularly coproporphyrinogen III, from the cytoplasm into mitochondria, a key step in heme synthesis. Additionally, ABCB6 is implicated in the trafficking of toxic metabolites, such as heavy metal-glutathione complexes, to lysosomes for sequestration or excretion, highlighting its role in cellular stress responses.
Recombinant ABCB6 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure, function, and interactions. Purified recombinant ABCB6 retains ATPase activity and substrate-binding properties, enabling in vitro investigations into its transport mechanisms. Studies leveraging this protein have uncovered its involvement in pathological conditions, including iron-deficiency anemia, erythropoietic protoporphyria, and chemoresistance in cancers. ABCB6 overexpression in tumors has been linked to multidrug resistance by effluxing chemotherapeutic agents, making it a potential therapeutic target. Furthermore, mutations in the ABCB6 gene are associated with dysregulated heme metabolism, leading to rare genetic disorders. The development of recombinant ABCB6 has accelerated drug discovery efforts, allowing high-throughput screening of inhibitors or modulators to combat ABCB6-mediated resistance or metabolic dysfunctions. Ongoing research aims to resolve its crystal structure and elucidate substrate specificity, which could inform precision therapies targeting this transporter.
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