| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCB10 |
| Uniprot No | Q9NRK6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-738aa |
| 氨基酸序列 | MRGPPAWPLRLLEPPSPAEPGRLLPVACVWAAASRVPGSLSPFTGLRPARLWGAGPALLWGVGAARRWRSGCRGGGPGASRGVLGLARLLGLWARGPGSCRCGAFAGPGAPRLPRARFPGGPAAAAWAGDEAWRRGPAAPPGDKGRLRPAAAGLPEARKLLGLAYPERRRLAAAVGFLTMSSVISMSAPFFLGKIIDVIYTNPTVDYSDNLTRLCLGLSAVFLCGAAANAIRVYLMQTSGQRIVNRLRTSLFSSILRQEVAFFDKTRTGELINRLSSDTALLGRSVTENLSDGLRAGAQASVGISMMFFVSPNLATFVLSVVPPVSIIAVIYGRYLRKLTKVTQDSLAQATQLAEERIGNVRTVRAFGKEMTEIEKYASKVDHVMQLARKEAFARAGFFGATGLSGNLIVLSVLYKGGLLMGSAHMTVGELSSFLMYAFWVGISIGGLSSFYSELMKGLGAGGRLWELLEREPKLPFNEGVILNEKSFQGALEFKNVHFAYPARPEVPIFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQLNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEKGVLLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTIKNANMVAVLDQGKITEYGKHEELLSKPNGIYRKLMNKQSFISA |
| 预测分子量 | 79,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCB10重组蛋白的3篇参考文献示例(注：文献信息为示例性内容，实际引用需核实原文)：
1. **文献名称**: *"ABCB10 mediates mitochondrial transport of heme precursors in human cells"*
**作者**: Chen W, et al.
**摘要**: 该研究通过重组ABCB10蛋白，揭示了其在人细胞线粒体膜上转运血红素前体(如原卟啉IX)的分子机制，并证明其缺失导致血红素合成障碍和线粒体功能异常。
2. **文献名称**: *"Structural insights into the ABCB10 transporter in lipid nanodiscs"*
**作者**: Kim H, et al.
**摘要**: 作者利用重组ABCB10蛋白结合脂质纳米盘技术解析了其高分辨率结构，阐明了ATP结合域构象变化与底物转运的偶联机制，为靶向ABCB10的药物设计提供结构基础。
3. **文献名称**: *"ABCB10 deficiency exacerbates oxidative stress in cardiac cells"*
**作者**: Zhang Y, et al.
**摘要**: 研究通过重组ABCB10蛋白的功能回补实验，证实其通过调控线粒体活性氧(ROS)清除系统(如谷胱甘肽代谢)保护心肌细胞免受氧化损伤，提示其与心血管疾病的相关性。
*注：以上文献信息为模拟生成，实际研究中建议通过PubMed或Web of Science以“ABCB10 recombinant protein”为关键词检索最新文献。*
ABCB10. also known as ATP-binding cassette subfamily B member 10. is a mitochondrial inner membrane protein belonging to the ABC transporter superfamily. It plays critical roles in cellular homeostasis, particularly in heme biosynthesis, iron metabolism, and protection against oxidative stress. Structurally, ABCB10 contains characteristic nucleotide-binding domains (NBDs) that hydrolyze ATP to drive substrate transport, though its exact physiological substrates remain under investigation. Studies suggest it may function as a chaperone or transporter coordinating heme synthesis with mitochondrial iron availability.
Recombinant ABCB10 protein is engineered through heterologous expression systems (e.g., Escherichia coli, mammalian cells) to study its molecular mechanisms and structural features. This engineered protein typically retains key functional domains, including transmembrane helices and ATPase motifs, enabling in vitro analysis of its transport activity and interactions with partner proteins like mitoferrin-1 in mitochondrial iron handling.
Research applications focus on understanding ABCB10's role in diseases linked to mitochondrial dysfunction, such as sideroblastic anemia, neurodegenerative disorders, and cancer. Its overexpression in certain tumors has sparked interest in developing ABCB10 inhibitors as potential therapeutic agents. Recombinant ABCB10 also serves as a tool for drug screening and deciphering molecular pathways involving heme-regulated processes. Despite progress, challenges persist in fully characterizing its transport mechanism and physiological partners, highlighting the continued importance of recombinant protein studies in elucidating ABCB10's multifaceted biological roles.
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