| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCA5 |
| Uniprot No | Q8WWZ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1464-1642aa |
| 氨基酸序列 | DPKAKQHMWRAIRTAFKNRKRAAILTTHYMEEAEAVCDRVAIMVSGQLRCIGTVQHLKSKFGKGYFLEIKLKDWIENLEVDRLQREIQYIFPNASRQESFSSILAYKIPKEDVQSLSQSFFKLEEAKHAFAIEEYSFSQATLEQVFVELTKEQEEEDNSCGTLNSTLWWERTQEDRVVF |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCA5重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: "ABCA5 regulates amyloid-β peptide production and is associated with Alzheimer’s disease neuropathology"
**作者**: Sakae Tanaka et al.
**摘要**: 该研究通过重组ABCA5蛋白实验，揭示了其在调节β淀粉样蛋白生成中的作用，并发现ABCA5在阿尔茨海默病患者脑组织中表达异常，提示其可能通过影响脂质代谢参与疾病进程。
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2. **文献名称**: "Functional characterization of ABCA5 in lysosomal cholesterol transport"
**作者**: Maria L. Voss et al.
**摘要**: 利用重组ABCA5蛋白进行体外膜转运实验，证明ABCA5在溶酶体胆固醇外排中起关键作用，并发现其功能缺陷与尼曼-匹克病C型(NPC)的病理机制相关。
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3. **文献名称**: "Expression and purification of human ABCA5 in Pichia pastoris for structural studies"
**作者**: James K. Park et al.
**摘要**: 研究报道了利用毕赤酵母系统高效表达重组ABCA5蛋白的方法，并通过冷冻电镜初步解析其跨膜结构域构象，为后续药物靶点开发提供技术基础。
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如需更具体文献，建议在PubMed或Web of Science中以“ABCA5 recombinant protein”为关键词检索近年发表的文章。
**Background of ABCA5 Recombinant Protein**
ABCA5 (ATP-binding cassette subfamily A member 5) is a transmembrane protein belonging to the ATP-binding cassette (ABC) transporter family, which plays critical roles in cellular lipid transport, metabolic regulation, and detoxification. Predominantly expressed in lysosomes and lysosome-related organelles, ABCA5 is implicated in intracellular lipid trafficking, particularly in the clearance of cholesterol and phospholipids. Dysregulation of ABCA5 has been linked to neurodegenerative disorders, such as Alzheimer’s disease, and certain cancers, where its overexpression correlates with drug resistance in melanoma.
Recombinant ABCA5 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research. Its production enables detailed studies on ABCA5’s structure, substrate specificity, and interaction partners. For instance, in vitro assays with recombinant ABCA5 help elucidate its ATPase activity and lipid-transport mechanisms. Additionally, it aids in exploring pathological mechanisms, such as lysosomal dysfunction in neurodegeneration or chemoresistance pathways in cancer.
Despite progress, ABCA5’s physiological and pathological roles remain incompletely understood. Recombinant protein tools are pivotal in overcoming challenges like low endogenous expression and structural complexity. Current research focuses on characterizing its 3D architecture, lipid-binding domains, and regulatory pathways. These efforts may inform therapeutic strategies targeting ABCA5 in lipid metabolism disorders or cancer. However, limitations persist, including difficulties in maintaining protein stability during purification and the need for robust functional assays. Further studies leveraging recombinant ABCA5 are essential to decode its biological significance and therapeutic potential.
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