| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCF1 |
| Uniprot No | Q8NE71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-845aa |
| 氨基酸序列 | MPKAPKQQPPEPEWIGDGESTSPSDKVVKKGKKDKKIKKTFFEELAVEDKQAGEEEKVLKEKEQQQQQQQQQQKKKRDTRKGRRKKDVDDDGEEKELMERLKKLSVPTSDEEDEVPAPKPRGGKKTKGGNVFAALIQDQSEEEEEEEKHPPKPAKPEKNRINKAVSEEQQPALKGKKGKEEKSKGKAKPQNKFAALDNEEEDKEEEIIKEKEPPKQGKEKAKKAEQGSEEEGEGEEEEEEGGESKADDPYAHLSKKEKKKLKKQMEYERQVASLKAANAAENDFSVSQAEMSSRQAMLENASDIKLEKFSISAHGKELFVNADLYIVAGRRYGLVGPNGKGKTTLLKHIANRALSIPPNIDVLLCEQEVVADETPAVQAVLRADTKRLKLLEEERRLQGQLEQGDDTAAERLEKVYEELRATGAAAAEAKARRILAGLGFDPEMQNRPTQKFSGGWRMRVSLARALFMEPTLLMLDEPTNHLDLNAVIWLNNYLQGWRKTLLIVSHDQGFLDDVCTDIIHLDAQRLHYYRGNYMTFKKMYQQKQKELLKQYEKQEKKLKELKAGGKSTKQAEKQTKEALTRKQQKCRRKNQDEESQEAPELLKRPKEYTVRFTFPDPPPLSPPVLGLHGVTFGYQGQKPLFKNLDFGIDMDSRICIVGPNGVGKSTLLLLLTGKLTPTHGEMRKNHRLKIGFFNQQYAEQLRMEETPTEYLQRGFNLPYQDARKCLGRFGLESHAHTIQICKLSGGQKARVVFAELACREPDVLILDEPTNNLDIESIDALGEAINEYKGAVIVVSHDARLITETNCQLWVVEEQSVSQIDGDFEDYKREVLEALGEVMVSRPRE |
| 预测分子量 | 95,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCF1重组蛋白的假设性参考文献示例(仅供参考，具体文献需通过学术数据库检索获取)：
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1. **文献名称**: *Functional characterization of recombinant ABCF1 protein in bacterial antibiotic resistance*
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化ABCF1重组蛋白，通过体外ATP酶活性实验证实其具有ATP结合能力，并发现ABCF1通过调控核糖体功能参与细菌四环素耐药性机制。
2. **文献名称**: *Crystal structure analysis of human ABCF1 reveals nucleotide-dependent conformational changes*
**作者**: Brown K, et al.
**摘要**: 作者解析了重组人源ABCF1蛋白的晶体结构，揭示了其ATP结合结构域的构象变化规律，为理解ABCF1在真核细胞翻译调控及免疫应答中的作用提供结构基础。
3. **文献名称**: *ABCF1 recombinant protein interacts with viral RNA to modulate host innate immunity*
**作者**: Lee S, et al.
**摘要**: 通过体外重组蛋白结合实验，证明ABCF1可直接结合病毒RNA并激活干扰素信号通路，提示其在抗病毒天然免疫中的潜在调控功能。
4. **文献名称**: *High-yield expression and purification of ABCF1 in insect cells for drug screening*
**作者**: Wang Y, et al.
**摘要**: 报道利用杆状病毒-昆虫细胞系统高效表达ABCF1重组蛋白，建立纯化工艺并用于小分子抑制剂筛选，为靶向ABCF1的抗菌药物开发提供平台。
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**注意**：以上文献为示例性质，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(可尝试关键词："ABCF1 recombinant protein" "expression" "structure" "antibiotic resistance")。
**Background of ABCF1 Recombinant Protein**
ABCF1 (ATP-binding cassette sub-family F member 1) is a critical protein belonging to the ATP-binding cassette (ABC) superfamily, which is evolutionarily conserved across eukaryotes. Unlike canonical ABC transporters, ABCF1 lacks transmembrane domains and does not participate in substrate translocation. Instead, it functions as a cytosolic ATPase involved in regulating translation and immune responses. Studies highlight its role in modulating ribosome function, where it interacts with eukaryotic translation initiation factors (e.g., eIF2 and eIF5) to influence translation initiation or elongation under stress conditions.
ABCF1 is also implicated in innate immunity, particularly in antiviral defense. It acts as a negative regulator of the integrated stress response (ISR) and interacts with viral RNA sensors like DDX3X, potentially inhibiting viral replication. Dysregulation of ABCF1 has been linked to autoimmune diseases, cancer progression, and viral infections, underscoring its therapeutic relevance.
Recombinant ABCF1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It typically retains key domains: two nucleotide-binding domains (NBDs) with conserved Walker motifs for ATP hydrolysis and a unique N-terminal regulatory region. Tags (e.g., His-tag) are often added for purification and detection.
Applications include *in vitro* studies on translation mechanisms, drug screening for ABCF1-targeted therapies (e.g., cancer or antiviral agents), and structural analyses (e.g., X-ray crystallography) to elucidate its molecular interactions. ABCF1 recombinant tools are vital for deciphering its dual roles in proteostasis and immunity, offering pathways for novel biomedical interventions.
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