| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCA9 |
| Uniprot No | Q8IUA7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1624aa |
| 氨基酸序列 | MSKRRMSVGQQTWALLCKNCLKKWRMKRQTLLEWLFSFLLVLFLYLFFSNLHQVHDTPQMSSMDLGRVDSFNDTNYVIAFAPESKTTQEIMNKVASAPFLKGRTIMGWPDEKSMDELDLNYSIDAVRVIFTDTFSYHLKFSWGHRIPMMKEHRDHSAHCQAVNEKMKCEGSEFWEKGFVAFQAAINAAIIEIATNHSVMEQLMSVTGVHMKILPFVAQGGVATDFFIFFCIISFSTFIYYVSVNVTQERQYITSLMTMMGLRESAFWLSWGLMYAGFILIMATLMALIVKSAQIVVLTGFVMVFTLFLLYGLSLITLAFLMSVLIKKPFLTGLVVFLLIVFWGILGFPALYTRLPAFLEWTLCLLSPFAFTVGMAQLIHLDYDVNSNAHLDSSQNPYLIIATLFMLVFDTLLYLVLTLYFDKILPAEYGHRCSPLFFLKSCFWFQHGRANHVVLENETDSDPTPNDCFEPVSPEFCGKEAIRIKNLKKEYAGKCERVEALKGVVFDIYEGQITALLGHSGAGKTTLLNILSGLSVPTSGSVTVYNHTLSRMADIENISKFTGFCPQSNVQFGFLTVKENLRLFAKIKGILPHEVEKEVQRVVQELEMENIQDILAQNLSGGQNRKLTFGIAILGDPQVLLLDEPTAGLDPLSRHRIWNLLKEGKSDRVILFSTQFIDEADILADRKVFISNGKLKCAGSSLFLKKKWGIGYHLSLHLNERCDPESITSLVKQHISDAKLTAQSEEKLVYILPLERTNKFPELYRDLDRCSNQGIEDYGVSITTLNEVFLKLEGKSTIDESDIGIWGQLQTDGAKDIGSLVELEQVLSSFHETRKTISGVALWRQQVCAIAKVRFLKLKKERKSLWTILLLFGISFIPQLLEHLFYESYQKSYPWELSPNTYFLSPGQQPQDPLTHLLVINKTGSTIDNFLHSLRRQNIAIEVDAFGTRNGTDDPSYNGAIIVSGDEKDHRFSIACNTKRLNCFPVLLDVISNGLLGIFNSSEHIQTDRSTFFEEHMDYEYGYRSNTFFWIPMAASFTPYIAMSSIGDYKKKAHSQLRISGLYPSAYWFGQALVDVSLYFLILLLMQIMDYIFSPEEIIFIIQNLLIQILCSIGYVSSLVFLTYVISFIFRNGRKNSGIWSFFFLIVVIFSIVATDLNEYGFLGLFFGTMLIPPFTLIGSLFIFSEISPDSMDYLGASESEIVYLALLIPYLHFLIFLFILRCLEMNCRKKLMRKDPVFRISPRSNAIFPNPEEPEGEEEDIQMERMRTVNAMAVRDFDETPVIIASCLRKEYAGKKKNCFSKRKKKIATRNVSFCVKKGEVIGLLGHNGAGKSTTIKMITGDTKPTAGQVILKGSGGGEPLGFLGYCPQENALWPNLTVRQHLEVYAAVKGLRKGDAMIAITRLVDALKLQDQLKAPVKTLSEGIKRKLCFVLSILGNPSVVLLDEPSTGMDPEGQQQMWQVIRATFRNTERGALLTTHYMAEAEAVCDRVAIMVSGRLRCIGSIQHLKSKFGKDYLLEMKLKNLAQMEPLHAEILRLFPQAAQQERFSSLMVYKLPVEDVRPLSQAFFKLEIVKQSFDLEEYSLSQSTLEQVFLELSKEQELGDLEEDFDPSVKWKLLLQEEP |
| 预测分子量 | 184,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCA9重组蛋白的3篇参考文献示例：
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1. **文献名称**：*"Functional characterization of ABCA9 recombinant protein in lipid transport"*
**作者**：Tanaka K, et al.
**摘要**：本研究成功在大肠杆菌系统中表达并纯化了ABCA9重组蛋白，验证其ATP酶活性及对胆固醇转运的功能。实验表明ABCA9可能参与细胞内脂质代谢调控，为研究其病理机制提供基础。
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2. **文献名称**：*"Structural analysis of ABCA9 using recombinant expression in HEK293 cells"*
**作者**：Smith JL, et al.
**摘要**：通过HEK293细胞表达ABCA9重组蛋白，结合冷冻电镜技术解析其三维结构，揭示了其跨膜结构域与底物结合口袋的独特特征，为靶向药物设计提供结构依据。
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3. **文献名称**：*"ABCA9 gene variants and their impact on recombinant protein stability"*
**作者**：Chen R, et al.
**摘要**：分析了ABCA9基因突变对重组蛋白稳定性的影响，发现特定突变导致蛋白降解加速，提示遗传变异可能通过影响ABCA9功能参与相关代谢疾病。
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注：以上文献信息为示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。若研究较少，可扩展至ABCA家族其他成员或相关功能研究。
**Background of ABCA9 Recombinant Protein**
The ATP-binding cassette subfamily A member 9 (ABCA9) is a transmembrane protein belonging to the ABC transporter superfamily, which plays critical roles in lipid metabolism, cellular homeostasis, and substrate transport across biological membranes. ABCA9 shares structural homology with other ABCA members, characterized by conserved nucleotide-binding domains (NBDs) that hydrolyze ATP to drive substrate translocation. While its precise physiological function remains under investigation, ABCA9 is hypothesized to participate in intracellular lipid trafficking, particularly in cholesterol homeostasis or lipid droplet regulation, based on its expression patterns in lipid-rich tissues like the liver, brain, and adipose tissue.
ABCA9 has garnered attention due to its potential links to metabolic disorders and cardiovascular diseases. Studies suggest its expression may be modulated by lipid-related transcription factors, such as liver X receptors (LXRs), implicating it in lipid storage or efflux pathways. Dysregulation of ABCA9 has been observed in conditions like atherosclerosis and obesity, though mechanistic insights remain limited.
Recombinant ABCA9 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect, or bacterial cells) to enable functional and structural studies. This purified protein facilitates research into its biochemical properties, substrate specificity, and interaction partners. Additionally, it serves as a tool for developing antibodies or screening therapeutic compounds targeting ABCA9-associated pathways.
Despite progress, many aspects of ABCA9 biology—including its exact substrates, regulatory mechanisms, and disease relevance—require further exploration. Recombinant ABCA9 protein thus represents a vital resource for unraveling its role in health and disease, with potential applications in diagnostics and drug discovery.
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