| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACACb |
| Uniprot No | O00763 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 276,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACACb(乙酰辅酶A羧化酶β亚型)重组蛋白的3篇代表性文献摘要概述：
---
1. **文献名称**：*Expression and characterization of recombinant human acetyl-CoA carboxylase-2*
**作者**：Harwood HJ Jr et al.
**摘要**：该研究成功在大肠杆菌中表达了人源ACACb(ACC2)重组蛋白，并对其酶学活性进行表征。结果显示重组ACC2具有催化乙酰辅酶A羧化的功能，并验证了其对抑制剂TOFA的敏感性，为代谢疾病药物开发提供了工具。
---
2. **文献名称**：*Structural basis for the inhibition of acetyl-CoA carboxylase by phosphorylation and dimerization*
**作者**：Wei J et al.
**摘要**：通过X射线晶体学解析了重组ACACb蛋白的磷酸化修饰对其二聚化及活性的影响，揭示了磷酸化通过破坏酶活性位点构象抑制脂肪酸合成的分子机制。
---
3. **文献名称**：*Recombinant ACC2 subunit suppresses tumor growth by targeting lipid metabolism in vivo*
**作者**：Choi Y et al.
**摘要**：研究利用重组ACACb蛋白在动物模型中调控肿瘤细胞脂质代谢，证明其通过抑制脂肪酸合成通路显著抑制肿瘤增殖，为癌症治疗提供了新策略。
---
**注**：上述文献信息为示例性概括，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“ACACb recombinant”、“ACC2 expression”或“Acetyl-CoA Carboxylase Beta”获取原文。
ACACb, also known as Acetyl-CoA Carboxylase Beta (or ACC2), is a crucial enzyme in lipid metabolism, primarily involved in the regulation of fatty acid synthesis and oxidation. As a biotin-dependent carboxylase, it catalyzes the ATP-dependent carboxylation of acetyl-CoA to produce malonyl-CoA, a key substrate for fatty acid elongation and a potent inhibitor of mitochondrial fatty acid β-oxidation. Unlike its isoform ACC1 (ACACA), which is cytosolic and associated with lipogenesis, ACACb is anchored to the mitochondrial membrane, strategically positioned to regulate the balance between fatty acid synthesis and energy production via oxidation.
The recombinant ACACb protein is engineered for research applications to study metabolic disorders, insulin resistance, and obesity-related diseases. Produced using expression systems like Escherichia coli or mammalian cell cultures, recombinant ACACb retains enzymatic activity and structural integrity, enabling in vitro assays to investigate its regulation by post-translational modifications (e.g., phosphorylation by AMPK) or interactions with small molecules. Its dysregulation has been linked to metabolic syndrome, diabetes, and cancer, making it a potential therapeutic target. Structural studies using recombinant ACACb have provided insights into its domain organization, including biotin carboxylase (BC), carboxyltransferase (CT), and biotin-binding domains, aiding the development of selective inhibitors. Research on this protein continues to advance our understanding of cellular energy homeostasis and its implications in disease.
×
