| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GALP |
| Uniprot No | Q9UBC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-84aa |
| 氨基酸序列 | APAHRG RGGWTLNSAG YLLGPVLHLP QMGDQDGKRE TALEILDLWK AIDGLPYSHP PQPS |
| 预测分子量 | 12,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GALP(galanin-like peptide)重组蛋白研究的3篇示例参考文献(内容为模拟概括，供参考)：
---
1. **文献名称**：*Expression and Purification of Recombinant Human GALP in E. coli for Functional Studies*
**作者**：Yada T. et al.
**摘要**：本研究利用大肠杆菌表达系统成功重组表达了人源GALP蛋白，并通过色谱技术纯化获得高纯度产物。体外实验证实重组GALP能激活下丘脑神经元中的相关受体，并显著抑制动物模型的摄食行为，提示其在代谢调控中的潜在作用。
---
2. **文献名称**：*Structural Characterization of GALP by NMR Reveals Key Domains for Receptor Binding*
**作者**：Smith J.R., Brown K.L.
**摘要**：通过核磁共振(NMR)技术解析重组GALP的三维结构，发现其N端区域为受体结合的关键结构域。该研究为基于GALP结构的神经调节类药物设计提供了分子基础。
---
3. **文献名称**：*Recombinant GALP Ameliorates Insulin Resistance in Obese Mouse Models*
**作者**：Chen L. et al.
**摘要**：在肥胖小鼠模型中，注射重组GALP显著改善了胰岛素敏感性和血糖水平，机制可能与抑制炎症因子释放及调节脂肪代谢相关。研究支持GALP在治疗代谢综合征中的应用潜力。
---
注：以上文献为示例，实际引用时需以真实发表的论文为准。如需查找真实文献，建议通过PubMed、Web of Science等平台以关键词“GALP recombinant protein”或“galanin-like peptide expression”检索。
**Background of GALP Recombinant Protein**
Galanin-like peptide (GALP), a neuropeptide first identified in the porcine hypothalamus in 1999. belongs to the galanin family, sharing structural homology with galanin—a peptide involved in diverse physiological processes. GALP is encoded by the *GALP* gene and consists of 60 amino acids in humans, including a conserved region that binds to galanin receptors (GALR1. GALR2. GALR3). Unlike galanin, GALP exhibits selective affinity for GALR2 and GALR3. enabling distinct regulatory roles in energy homeostasis, reproduction, and immune response.
The production of GALP recombinant protein leverages genetic engineering to express and purify the peptide in heterologous systems, such as *E. coli*, yeast, or mammalian cells. This approach ensures high yield, purity, and consistency, addressing challenges in isolating native GALP from biological tissues. Recombinant GALP retains bioactivity, facilitating its use in studying signaling pathways, receptor interactions, and physiological effects.
Research highlights GALP's role in modulating appetite and metabolism via hypothalamic circuits, linking it to obesity and diabetes studies. It also influences reproductive functions by regulating gonadotropin-releasing hormone (GnRH) secretion. Moreover, GALP's anti-inflammatory properties suggest therapeutic potential in neurodegenerative and autoimmune diseases.
Despite progress, challenges remain in optimizing recombinant GALP stability, delivery, and receptor specificity. Advances in protein engineering, such as site-directed mutagenesis or fusion tags, aim to enhance its therapeutic applicability. Overall, GALP recombinant protein serves as a critical tool for unraveling neuroendocrine mechanisms and developing targeted therapies for metabolic and reproductive disorders.
×
