| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AGXT2 |
| Uniprot No | Q9BYV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-514aa |
| 氨基酸序列 | LHTKPRMPP CDFMPERYQS LGYNRVLEIH KEHLSPVVTA YFQKPLLLHQ GHMEWLFDAE GSRYLDFFSG IVTVSVGHCH PKVNAVAQKQ LGRLWHTSTV FFHPPMHEYA EKLAALLPEP LKVIFLVNSG SEANELAMLM ARAHSNNIDI ISFRGAYHGC SPYTLGLTNV GTYKMELPGG TGCQPTMCPD VFRGPWGGSH CRDSPVQTIR KCSCAPDCCQ AKDQYIEQFK DTLSTSVAKS IAGFFAEPIQ GVNGVVQYPK GFLKEAFELV RARGGVCIAD EVQTGFGRLG SHFWGFQTHD VLPDIVTMAK GIGNGFPMAA VITTPEIAKS LAKCLQHFNT FGGNPMACAI GSAVLEVIKE ENLQENSQEV GTYMLLKFAK LRDEFEIVGD VRGKGLMIGI EMVQDKISCR PLPREEVNQI HEDCKHMGLL VGRGSIFSQT FRIAPSMCIT KPEVDFAVEV FRSALTQHME RRAK |
| 预测分子量 | 57,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AGXT2重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: *"Expression, purification, and characterization of human alanine-glyoxylate aminotransferase 2 (AGXT2) in Escherichia coli"*
**作者**: E. M. Raimondi et al.
**摘要**: 该研究成功构建了人源AGXT2重组蛋白的原核表达系统，利用大肠杆菌进行表达并通过亲和层析纯化。酶活性分析表明重组AGXT2具有催化L-丙氨酸与乙醛酸转氨基反应的能力，并发现其活性受pH和金属离子的调控。
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2. **文献名称**: *"Functional analysis of AGXT2 polymorphisms associated with cardiovascular disease"*
**作者**: K. T. Suzuki et al.
**摘要**: 通过重组表达携带不同单核苷酸多态性(SNPs)的AGXT2突变体蛋白，研究发现某些SNPs(如rs37369)显著降低酶活性，可能导致内源性对称二甲基精氨酸(SDMA)代谢异常，从而与高血压和动脉硬化风险相关。
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3. **文献名称**: *"Structural insights into the substrate specificity of AGXT2 through recombinant protein crystallography"*
**作者**: L. J. Wang et al.
**摘要**: 利用重组AGXT2蛋白的结晶结构分析，揭示了其底物结合口袋的构象特征，阐明了该酶对特定氨基酸底物(如β-氨基异丁酸)的选择性催化机制，为设计靶向抑制剂提供了结构基础。
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4. **文献名称**: *"Role of AGXT2 in renal metabolism: Evidence from recombinant enzyme assays and knockout mice"*
**作者**: G. P. Roberts et al.
**摘要**: 通过重组AGXT2蛋白体外实验及基因敲除小鼠模型，证明AGXT2在肾脏中参与氧化应激代谢通路，其缺失导致血浆甲基胍水平升高，提示其在慢性肾病病理中的潜在作用。
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注：以上文献信息为示例，实际引用需根据具体论文内容调整。建议通过PubMed或Web of Science以“AGXT2 recombinant protein”为关键词检索最新研究。
**Background of AGXT2 Recombinant Protein**
Alanine-glyoxylate aminotransferase 2 (AGXT2) is a mitochondrial enzyme encoded by the *AGXT2* gene, primarily expressed in the liver and kidneys. It belongs to the pyridoxal 5'-phosphate (PLP)-dependent aminotransferase family and plays a role in metabolizing endogenous and exogenous compounds. AGXT2 catalyzes the transamination of glyoxylate to glycine using L-alanine as an amino donor, thereby modulating glyoxylate levels. Elevated glyoxylate can lead to oxalate accumulation, a risk factor for kidney stones and renal dysfunction.
AGXT2 also interacts with β-aminoisobutyric acid (BAIB), a metabolite involved in thymine and valine catabolism, influencing metabolic pathways linked to cardiovascular and metabolic disorders. Genetic variations in *AGXT2* have been associated with altered enzyme activity, impacting individual susceptibility to diseases like hypertension, atherosclerosis, and diabetes.
Recombinant AGXT2 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its purification enables research into substrate specificity, kinetic properties, and inhibitor screening. Studies leveraging recombinant AGXT2 aim to clarify its physiological roles, explore therapeutic targets for oxalate-related disorders, and investigate its connection to nitric oxide metabolism, as AGXT2 indirectly regulates nitric oxide synthase activity by metabolizing asymmetric dimethylarginine (ADMA), an endogenous inhibitor.
Overall, AGXT2 recombinant protein serves as a critical tool for understanding metabolic regulation, disease mechanisms, and potential drug development.
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