| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHAG |
| Uniprot No | Q02094 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409aa |
| 氨基酸序列 | MRFTFPLMAIVLEIAMIVLFGLFVEYETDQTVLEQLNITKPTDMGIFFELYPLFQDVHVMIFVGFGFLMTFLKKYGFSSVGINLLVAALGLQWGTIVQGILQSQGQKFNIGIKNMINADFSAATVLISFGAVLGKTSPTQMLIMTILEIVFFAHNEYLVSEIFKASDIGASMTIHAFGAYFGLAVAGILYRSGLRKGHENEESAYYSDLFAMIGTLFLWMFWPSFNSAIAEPGDKQCRAIVNTYFSLAACVLTAFAFSSLVEHRGKLNMVHIQNATLAGGVAVGTCADMAIHPFGSMIIGSIAGMVSVLGYKFLTPLFTTKLRIHDTCGVHNLHGLPGVVGGLAGIVAVAMGASNTSMAMQAAALGSSIGTAVVGGLMTGLILKLPLWGQPSDQNCYDDSVYWKVPKTR |
| 预测分子量 | 44,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RHAG重组蛋白的3篇参考文献及其摘要概括：
1. **《Structural basis of Rh antigen complex recognition by the RhAG protein》**
- 作者：Liu, W., et al.
- 摘要：通过冷冻电镜解析RhAG蛋白与Rh抗原复合物的结构，揭示RhAG在维持红细胞膜稳定性中的作用及其与溶血性贫血的关联。
2. **《Functional characterization of recombinant RHAG glycoprotein in vitro》**
- 作者：Westhoff, C.M., et al.
- 摘要：研究重组RHAG蛋白在哺乳动物细胞中的表达与功能，验证其在氨转运和维持红细胞形态中的关键作用，为遗传性溶血性疾病提供分子机制解释。
3. **《RHAG recombinant protein as a potential biomarker for hemolytic anemia》**
- 作者：Tournamille, C., et al.
- 摘要：探讨重组RHAG蛋白在溶血性贫血患者中的表达异常，提出其作为疾病诊断标志物的可能性，并通过体外实验验证其与红细胞膜缺陷的关联。
注：以上文献信息为示例性质，实际研究中请通过PubMed或Web of Science等数据库检索具体文献。
RHAG (Rh-associated glycoprotein) is a transmembrane protein encoded by the RHAG gene, belonging to the Rh protein family. It plays a critical role in maintaining erythrocyte membrane integrity and facilitating gas exchange. Structurally, RHAG forms a core component of the Rh membrane complex alongside RhD/RhCE proteins, which is essential for anchoring the erythrocyte cytoskeleton to the lipid bilayer. This interaction ensures red blood cell (RBC) deformability and osmotic resistance during circulation.
Biologically, RHAG functions as a gas channel, potentially enabling ammonium (NH₃/NH₄⁺) transport and CO₂ exchange, though its exact physiological substrates remain under investigation. Mutations in RHAG are linked to hereditary stomatocytoses, a group of hemolytic anemias characterized by abnormal RBC morphology and cation permeability. Notably, RHAG is distinct from Rh blood group antigens but shares ~40% sequence homology with RhD proteins.
Recombinant RHAG proteins are engineered using expression systems like mammalian cells or insect cells to preserve post-translational modifications and native conformation. These proteins serve as vital tools for studying erythrocyte membrane disorders, developing blood group serology reagents, and exploring novel therapeutic strategies for hemolytic conditions. Recent studies also investigate RHAG's potential roles in non-erythroid tissues, expanding its biomedical relevance beyond hematology. Its conserved expression across vertebrates underscores evolutionary significance in gas homeostasis and cellular resilience.
×
