| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR90 |
| Uniprot No | Q96KV7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1748aa |
| 氨基酸序列 | MARAWQHPFLNVFRHFRVDEWKRSAKQGDVAVVTDKTLKGAVYRIRGSVSAANYIQLPKSSTQSLGLTGRYLYVLFRPLPSKHFVIHLDVSSKDNQVIRVSFSNLFKEFKSTATWLQFPLVLEARTPQRDLVGLAPSGARWTCLQLDLQDVLLVYLNRCYGHLKSIRLCASLLVRNLYTSDLCFEPAISGAQWAKLPVTPMPREMAFPVPKGESWHDRYIHVRFPSESLKVPSKPIEKSCSPPEAVLLGPGPQPLPCPVASSKPVRFSVSPVVQTPSPTASGRAALAPRPFPEVSLSQERSDASNADGPGFHSLEPWAQLEASDIHTAAAGTHVLTHESAEVPVARTGSCEGFLPDPVLRLKGVIGFGGHGTRQALWTPDGAAVVYPCHAVIVVLLVDTGEQRFFLGHTDKVSALALDGSSSLLASAQARAPSVMRLWDFQTGRCLCLFRSPMHVVCSLSFSDSGALLCGVGKDHHGRTMVVAWGTGQVGLGGEVVVLAKAHTDFDVQAFRVTFFDETRMASCGQGSVRLWRLRGGVLRSCPVDLGEHHALQFTDLAFKQARDGCPEPSAAMLFVCSRSGHILEIDCQRMVVRHARRLLPTRTPGGPHPQKQTFSSGPGIAISSLSVSPAMCAVGSEDGFLRLWPLDFSSVLLEAEHEGPVSSVCVSPDGLRVLSATSSGHLGFLDTLSRVYHMLARSHTAPVLALAMEQRRGQLATVSQDRTVRIWDLATLQQLYDFTSSEDAPCAVTFHPTRPTFFCGFSSGAVRSFSLEAAEVLVEHTCHRGAVTGLTATPDGRLLFSSCSQGSLAQYSCADPQWHVLRVAADMVCPDAPASPSALAVSRDGRLLAFVGPSRCTVTVMGSASLDELLRVDIGTLDLASSRLDSAMAVCFGPAALGHLLVSTSSNRVVVLDAVSGRIIRELPGVHPEPCPSLTLSEDARFLLIAAGRTIKVWDYATQASPGPQVYIGHSEPVQAVAFSPDQQQVLSAGDAVFLWDVLAPTESDQSFPGAPPACKTGPGAGPLEDAASRASELPRQQVPKPCQASPPRLGVCARPPEGGDGARDTRNSGAPRTTYLASCKAFTPARVSCSPHSAKGTCPPPASGGWLRLKAVVGYSGNGRANMVWRPDTGFFAYTCGRLVVVEDLHSGAQQHWSGHSAEISTLALSHSAQVLASASGRSSTTAHCQIRVWDVSGGLCQHLIFPHSTTVLALAFSPDDRLLVTLGDHDGRTLALWGTATYDLVSSTRLPEPVHGVAFNPWDAGELTCVGQGTVTFWLLQQRGADISLQVRREPVPEAVGAGELTSLCYGAPPLLYCGTSSGQVCVWDTRAGRCFLSWEADDGGIGLLLFSGSRLVSGSSTGRLRLWAVGAVSELRCKGSGASSVFMEHELVLDGAVVSASFDDSVDMGVVGTTAGTLWFVSWAEGTSTRLISGHRSKVNEVVFSPGESHCATCSEDGSVRVWALASMELVIQFQVLNQSCLCLAWSPPCCGRPEQQRLAAGYGDGSLRIFSVSRTAMELKMHPHPVALTTVAFSTDGQTVLSGDKDGLVAVSHPCTGTTFRVLSDHQGAPISTICVTCKECEDLGVEGTDLWLAASGDQRVSVWASDWLRNHCELVDWLSFPMPATTETQGHLPPSLAAFCPWDGALLMYVGPGVYKEVIIYNLCQKQVVEKIPLPFFAMSLSLSPGTHLLAVGFAECMLRLVDCAMGTAQDFAGHDNAVHLCRFTPSARLLFTAARNEILVWEVPGL |
| 预测分子量 | 187,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于WDR90重组蛋白的参考文献示例(实际文献可能需要通过学术数据库查询)：
1. **"Expression and Purification of Recombinant WDR90 Protein in E. coli"**
*Author: Zhang L, et al. (2020)*
**摘要**：本研究成功构建了WDR90重组蛋白的原核表达系统，利用大肠杆菌进行高效表达，并通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **"Structural Characterization of WDR90 and Its Interaction with Tubulin"**
*Author: Kim S, et al. (2018)*
**摘要**：通过X射线晶体学解析了WDR90重组蛋白的三维结构，发现其WD40结构域与微管蛋白存在直接相互作用，提示其在细胞骨架调控中的潜在作用。
3. **"Functional Analysis of WDR90 in DNA Damage Response"**
*Author: Müller R, et al. (2021)*
**摘要**：利用重组WDR90蛋白进行体外生化实验，证实其参与DNA损伤修复通路，可能与ATM/ATR激酶信号传导相关。
4. **"WDR90 Knockdown and Recombinant Rescue in Ciliogenesis Models"**
*Author: Patel N, et al. (2019)*
**摘要**：研究通过重组WDR90蛋白回补实验，验证了WDR90在纤毛形成中的必要性，并揭示其与CEP290蛋白的共定位现象。
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**注意**：以上为模拟文献，实际研究请查询PubMed、Web of Science等平台。若WDR90相关研究较少，建议扩展检索其同源蛋白或通路关键词(如WD40蛋白家族、纤毛相关疾病等)。
WDR90 (WD Repeat Domain 90) is a protein encoded by the *WDR90* gene in humans, belonging to the WD40 repeat protein family. These proteins are characterized by tandem repeats of ~40 amino acids, often terminating with tryptophan-aspartic acid (WD) motifs, which facilitate protein-protein interactions and scaffold formation in multiprotein complexes. WDR90 is evolutionarily conserved across eukaryotes, suggesting a fundamental biological role. Although its precise molecular function remains under investigation, bioinformatic analyses and preliminary studies link WDR90 to ciliary biology, intracellular trafficking, and cell cycle regulation. It is predominantly localized to the cytoplasm and cilia, with potential involvement in maintaining ciliary structure or signaling pathways.
Recombinant WDR90 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to produce purified protein for functional and structural studies. This enables researchers to explore its binding partners, enzymatic activities, or regulatory roles. Structural studies using recombinant WDR90 could elucidate how its WD40 repeats mediate interactions, potentially forming circular β-propeller domains that serve as platforms for assembling signaling modules. Dysregulation of WDR90 has been tentatively associated with ciliopathies, a group of disorders linked to defective cilia, though direct evidence remains limited. Recent genomic studies also suggest possible ties to neurodevelopmental conditions and cancers, highlighting its biomedical relevance.
Research on recombinant WDR90 aims to clarify its role in cellular processes and disease mechanisms. Such work could inform therapeutic strategies targeting cilia-related disorders or pathways involving WD40 proteins. However, challenges persist in mapping its interactome and defining its precise contributions to cellular homeostasis, underscoring the need for further biochemical and in vivo studies.
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