| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCL1 |
| Uniprot No | Q15111 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1095aa |
| 氨基酸序列 | MAEGAAGREDPAPPDAAGGEDDPRVGPDAAGDCVTAASGGRMRDRRSGVALPGAAGTPADSEAGLLEAARATPRRSSIIKDPSNQKCGGRKKTVSFSSMPSEKKISSANDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADVDGNGIMLEDTSVELIKQLNPTLKEAKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVTHITEDICLDIIRRYELSEEGRQKGFLAIDGFTQYLLSSECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYIRALKMGCRSVELDVSDGSDNEPILCNRNNMTTHVSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKVMAQQMKKVFGNKLYTEAPLPSESYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRMSVDYNGEQKQIRLCRELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKKFLSRIYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGILPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPSVSLVMKDSFPYLEPLGAIPDVQKKMLTAYDLMIQESRFLIEMADTVQEKIVQCQKAGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAIENMKQIQLACLSCGLSKAPSSSAEAKSKRSLEAIEEKESSEENGKL |
| 预测分子量 | 122,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCL1重组蛋白的3篇示例参考文献(注：以下文献为虚构示例，供参考)：
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1. **文献名称**: *"Recombinant PLCL1 Protein Inhibits TGF-β Signaling and Attenuates Pulmonary Fibrosis"*
**作者**: Chen L, Wang H, Zhang Q, et al.
**摘要**: 研究通过在大肠杆菌中表达并纯化重组PLCL1蛋白，发现其能够结合TGF-β受体并抑制下游Smad信号通路，显著减轻小鼠模型中的肺纤维化病理进展。
2. **文献名称**: *"Structural and Functional Characterization of Human PLCL1 Recombinant Protein Expressed in HEK293 Cells"*
**作者**: Kim S, Ito T, Tanaka M
**摘要**: 利用哺乳动物HEK293细胞系统表达PLCL1重组蛋白，通过质谱和酶活性分析证实其具有磷脂酶C样活性，并揭示了其钙离子依赖性催化机制。
3. **文献名称**: *"PLCL1 Recombinant Protein Modulates Autophagy in Cardiomyocytes via mTOR Pathway"*
**作者**: Gupta R, Patel S, Lee JY
**摘要**: 研究显示，外源性重组PLCL1蛋白通过抑制mTOR通路激活心肌细胞自噬，改善缺血再灌注损伤模型中的细胞存活率，提示其在心血管疾病中的潜在治疗价值。
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**注**：若需真实文献，建议通过PubMed、Web of Science等学术数据库，以“PLCL1 recombinant protein”“PLCL1 expression”等关键词检索，并筛选涉及基因克隆、蛋白功能或疾病机制的研究。
PLCL1 (Phospholipase C-like 1) is a protein encoded by the *PLCL1* gene, belonging to the phospholipase C (PLC) family. Unlike canonical PLC enzymes, PLCL1 lacks catalytic activity due to missing key residues in its catalytic domain, suggesting a non-enzymatic regulatory role. It retains structural domains common to PLCs, including pleckstrin homology (PH), EF-hand, and C2 domains, which are implicated in membrane binding, calcium signaling, and protein-protein interactions. PLCL1 is ubiquitously expressed, with high levels in the brain, heart, and skeletal muscle, and is involved in intracellular signaling pathways regulating cell proliferation, differentiation, and apoptosis.
Research indicates PLCL1 interacts with signaling molecules like G proteins, receptors, and cytoskeletal proteins, acting as a scaffold or adaptor protein. Its dysfunction has been linked to pathologies such as cancer, neurological disorders, and cardiovascular diseases. For instance, PLCL1 hypermethylation or downregulation is observed in certain cancers, correlating with tumor progression and poor prognosis. In neuroscience, PLCL1 may modulate synaptic plasticity and neuronal survival.
Recombinant PLCL1 protein, produced via bacterial or mammalian expression systems, is a vital tool for studying its molecular functions. It enables in vitro analyses of binding partners, structural characterization, and pathway mapping. Purified PLCL1 is also used to generate antibodies for diagnostic assays or to explore therapeutic targeting. Despite progress, its precise mechanisms remain unclear, necessitating further research into its role in health and disease. Overall, PLCL1 represents a unique, catalytically inactive member of the PLC family with potential as a biomarker or therapeutic target in multiple diseases.
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