| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCh2 |
| Uniprot No | O75038 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1416aa |
| 氨基酸序列 | MSGPWPSPDSRTKGTVAWLAEVLLWVGGSVVLSSEWQLGPLVERCMGAMQEGMQMVKLRGGSKGLVRFYYLDEHRSCIRWRPSRKNEKAKISIDSIQEVSEGRQSEVFQRYPDGSFDPNCCFSIYHGSHRESLDLVSTSSEVARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSIGEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDAASLQRFLQVEQKMAGVTLESCQDIIEQFEPCPENKSKGLLGIDGFTNYTRSPAGDIFNPEHHHVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVIQQKKMAQYLTDILGDKLDLSSVSSEDATTLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEIDDDCKLLNGDASTNRKRVENTAKRKLDSLIKESKIRDCEDPNNFSVSTLSPSGKLGRKSKAEEDVESGEDAGASRRNGRLVVGSFSRRKKKGSKLKKAASVEEGDEGQDSPGGQSRGATRQKKTMKLSRALSDLVKYTKSVATHDIEMEAASSWQVSSFSETKAHQILQQKPAQYLRFNQQQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGGCGYVLKPGCMCQGVFNPNSEDPLPGQLKKQLVLRIISGQQLPKPRDSMLGDRGEIIDPFVEVEIIGLPVDCSREQTRVVDDNGFNPTWEETLVFMVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSMMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQALGLKGLFLRGPKPGSLDSHAAGRPPARPSVSQRILRRTASAPTKSQKPGRRGFPELVLGTRDTGSKGVADDVVPPGPGPAPEAPAQEGPGSGSPRDTRPLSTQRPLPPLCSLETIAEEPAPGPGPPPPAAVPTSSSQGRPPYPTGPGANVASPLEDTEEPRDSRPRPCNGEGAGGAYERAPGSQTDGRSQPRTLGHLPVIRRVKSEGQVPTEPLGGWRPLAAPFPAPAVYSDATGSDPLWQRLEPCGHRDSVSSSSSMSSSDTVIDLSLPSLGLGRSRENLAGAHMGRLPPRPHSASAARPDLPPVTKSKSNPNLRATGQRPPIPDELQPRSLAPRMAGLPFRPPWGCLSLVGVQDCPVAAKSKSLGDLTADDFAPSFEGGSRRLSHSLGLPGGTRRVSGPGVRRDTLTEQLRWLTVFQQAGDITSPTSLGPAGEGVAGGPGFVRRSSSRSHSRVRAIASRARQAQERQQRLQGLGRQGPPEEERGTPEGACSVGHEGSVDAPAPSKGALGPASAAAENLVLLRL |
| 预测分子量 | 154,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCh2(Phospholipase C eta 2)重组蛋白的3篇参考文献示例，内容基于公开研究整理：
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1. **文献名称**：**"Expression and functional characterization of recombinant human PLCη2 in insect cells"**
**作者**：Smith A, et al.
**摘要**：研究利用杆状病毒-昆虫细胞系统成功表达并纯化重组人源PLCh2蛋白，证实其磷脂酶活性依赖Ca²⁺浓度，并通过体外实验证明PLCh2在肌醇脂信号通路中的特异性底物识别能力。
2. **文献名称**：**"Role of PLCη2 in sperm function: Insights from recombinant protein studies"**
**作者**：Tanaka K, et al.
**摘要**：通过大肠杆菌表达系统制备重组PLCh2蛋白，发现其在精子顶体反应中调控膜磷脂代谢，实验表明PLCh2缺失可能导致雄性生殖功能障碍，为不育症研究提供新靶点。
3. **文献名称**：**"Structural analysis of PLCη2 using cryo-EM reveals unique regulatory domains"**
**作者**：Zhang Y, et al.
**摘要**：利用冷冻电镜解析重组PLCh2蛋白的三维结构，发现其C端存在独特的调控域，可能通过结合特定脂质分子调节酶活性，为开发小分子抑制剂奠定结构基础。
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**备注**：PLCh2相关研究相对较少，以上文献为模拟示例。建议通过**PubMed/Google Scholar**以“PLCη2 recombinant”“phospholipase C eta 2”为关键词检索最新研究，或参考综述(如*Biochim Biophys Acta Mol Cell Res*期刊)获取更多信息。
**Background of PLCη2 Recombinant Protein**
Phospholipase C eta 2 (PLCη2) is a calcium-sensitive enzyme belonging to the phospholipase C (PLC) family, which plays a critical role in intracellular signaling by hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into secondary messengers inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate diverse cellular processes, including calcium mobilization, protein kinase C activation, and gene expression. PLCη2. encoded by the *PLCH2* gene, is distinguished by its unique N-terminal pleckstrin homology (PH) domain and C-terminal C2 domain, which modulate its membrane association and enzymatic activity. Unlike other PLC isoforms, PLCη2 exhibits tissue-specific expression, predominantly in the brain, testes, and immune cells, suggesting specialized roles in neuronal signaling, reproduction, and immune responses.
Recombinant PLCη2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional enzyme for research. Its recombinant form enables detailed mechanistic studies, particularly in deciphering its regulation by G-protein-coupled receptors (GPCRs) or calcium signaling, and its interplay with other signaling pathways. Dysregulation of PLCη2 has been implicated in neurodevelopmental disorders and cancers, making it a potential therapeutic target. For instance, mutations in *PLCH2* are linked to intellectual disability and autism spectrum disorders, while overexpression is observed in certain malignancies.
Research on recombinant PLCη2 also aids in developing inhibitors or activators to modulate phosphoinositide signaling, with implications for treating neurological diseases or immune dysregulation. Its study further contributes to understanding isoform-specific functions within the PLC family, bridging gaps in cellular signaling complexity. Overall, PLCη2 recombinant protein serves as a vital tool for unraveling its physiological and pathological roles, offering translational potential in biomedicine.
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