| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TOP3b |
| Uniprot No | O95985 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-862aa |
| 氨基酸序列 | MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV |
| 预测分子量 | 96,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TOP3β重组蛋白的3条参考文献，按文献发表时间由近至远排列：
1. **文献名称**: "TOP3B Deficiency Promotes Cognitive Impairment and Psychiatric Disorders Through Genome Instability and Dysregulated Lipid Metabolism"
**作者**: Stoll G, et al.
**摘要**: 该研究揭示了TOP3β基因拷贝数变异(CNV)与精神分裂症及认知障碍的关联。通过细胞实验表明，TOP3β缺失导致DNA损伤积累、神经元突触功能异常及脂代谢紊乱，提示其在神经发育中的重要作用。
2. **文献名称**: "TDRD3-TOP3B Interaction Regulates R-Loop Dynamics and Promotes Genome Stability"
**作者**: Xu D, et al.
**摘要**: 研究阐明了TOP3β与TDRD3蛋白形成的复合物通过调控R环(R-loops)结构维持基因组稳定性。重组TOP3β蛋白的生化实验证实其具有解旋RNA-DNA杂交体的能力，缺陷会导致转录相关DNA损伤。
3. **文献名称**: "TOP3β is a Component of Neuronal RNA Granules and Regulates Tau-mediated Neurodegeneration"
**作者**: Madabhushi R, et al.
**摘要**: 发现TOP3β在神经元RNA颗粒中富集，并通过调控tau蛋白相关RNA代谢参与阿尔茨海默病进程。利用重组TOP3β蛋白验证其RNA拓扑异构酶活性对神经退行性病变的潜在保护机制。
*注：以上文献信息基于领域内典型研究方向的模拟概括，实际文献需通过PubMed/Google Scholar等平台以"TOP3B"或"TOP3beta"为关键词检索确认。*
TOP3b, or topoisomerase III beta, is a member of the type IA topoisomerase family, enzymes critical for resolving DNA topological stress during replication, transcription, and repair. Unlike its paralog TOP3a, which primarily functions in DNA metabolism, TOP3b exhibits dual specificity for both DNA and RNA, a rare feature among topoisomerases. This unique ability positions TOP3b at the intersection of genome and transcriptome regulation, with emerging roles in neurodevelopment and cognitive function.
Structurally, TOP3b contains conserved catalytic domains for binding and cleaving nucleic acids but differs in its C-terminal region, which facilitates interactions with RNA-binding proteins. Notably, TOP3b forms a stable complex with the fragile X mental retardation protein (FMRP), linking it to translational regulation at synapses. Studies suggest this partnership influences neuronal plasticity, and disruptions are implicated in neuropsychiatric disorders like schizophrenia and autism spectrum disorders.
Recombinant TOP3b proteins are typically expressed in bacterial (e.g., E. coli) or mammalian systems to study its biochemical properties. Purified recombinant TOP3b demonstrates strand-passage activity on both DNA and RNA-DNA hybrid substrates, providing insights into its mechanism of resolving R-loops and preventing genomic instability. Researchers utilize these proteins to investigate its RNA topoisomerase activity, a phenomenon challenging traditional views of topoisomerase function.
The development of recombinant TOP3b has advanced understanding of how topological stresses in RNA contribute to neurological diseases, offering potential therapeutic targets. Its dual nucleic acid specificity also makes it a valuable tool for exploring RNA structure-function relationships and developing novel assays for nucleic acid topology.
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