| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H6 |
| Uniprot No | Q7KZ85 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1726aa |
| 氨基酸序列 | MSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIFQDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR |
| 预测分子量 | 199 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H6重组蛋白的3篇代表性文献概览，涵盖表达、结构及免疫应用研究：
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1. **文献名称**：*Expression and purification of H6 subtype influenza virus hemagglutinin for vaccine development*
**作者**：Smith A, et al.
**摘要**：本研究在杆状病毒/昆虫细胞系统中成功表达了H6N1亚型流感病毒的血凝素(HA)重组蛋白，通过亲和层析纯化获得高纯度产物。动物实验表明，该重组H6蛋白能诱导小鼠产生中和抗体，为H6亚型流感疫苗研发提供了候选抗原。
2. **文献名称**：*Structural characterization of H6 tagged recombinant SARS-CoV-2 spike protein using cryo-EM*
**作者**：Chen L, et al.
**摘要**：作者利用哺乳动物表达系统制备了携带His6标签的新冠病毒刺突蛋白(S蛋白)重组体，并通过冷冻电镜解析其三维结构。研究揭示了S蛋白与ACE2受体的结合域构象变化，为抗病毒药物设计提供了结构基础。
3. **文献名称**：*Optimization of H6-fusion protein expression in E. coli for diagnostic assay development*
**作者**：Wang X, et al.
**摘要**：通过密码子优化和诱导条件筛选，实现了结核分枝杆菌抗原H6重组蛋白在大肠杆菌中的高效可溶性表达。纯化后的蛋白在ELISA检测中表现出高灵敏度与特异性，证实其作为血清学诊断试剂的潜力。
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**注**：以上文献为示例性概括，实际引用时需核实具体文献信息及准确性。若需扩展，可进一步检索PubMed、Google Scholar等平台，关键词如“H6 recombinant protein expression”“H6 hemagglutinin vaccine”等。
**Background of H6 Recombinant Protein**
The H6 recombinant protein is a genetically engineered biomolecule derived from the hemagglutinin (H) subunit of influenza A viruses, specifically the H6 subtype. Hemagglutinin, a surface glycoprotein, plays a critical role in viral entry by binding to host cell receptors and facilitating membrane fusion. The H6 subtype is predominantly found in avian influenza viruses, particularly in wild and domestic birds, and has occasionally been detected in mammals, raising concerns about its zoonotic potential. While H6 viruses are considered low-pathogenic in poultry, their ability to reassort with other influenza strains highlights a risk for generating novel variants with pandemic capabilities.
Recombinant H6 protein is produced using biotechnological platforms, such as baculovirus-insect cell systems or mammalian cell cultures, which enable precise control over protein structure and post-translational modifications. This approach ensures high purity and antigenic fidelity, making the protein valuable for research and diagnostic applications. For instance, H6 recombinant proteins are widely used in serological assays to detect H6-specific antibodies in surveillance studies, aiding in tracking viral spread and evolution.
In vaccine development, recombinant H6 proteins serve as candidate antigens to explore cross-protective immunity. Studies have shown that H6-based vaccines can elicit robust antibody responses in animal models, though their efficacy in humans remains under investigation. Additionally, structural studies of H6 hemagglutinin contribute to understanding receptor-binding preferences and mechanisms of host adaptation, which are critical for assessing pandemic risks.
Recent advances in protein engineering, such as nanoparticle display or stabilized pre-fusion conformations, have further enhanced the utility of H6 recombinant proteins in both basic virology and applied immunology. Despite progress, challenges persist in optimizing scalability and cost-effectiveness for large-scale production. Overall, H6 recombinant proteins represent a vital tool for influenza research, bridging gaps between viral ecology, pathogenesis, and preventive strategies.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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