| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GLVR1 |
| Uniprot No | Q8WUM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679aa |
| 氨基酸序列 | MATLITSTTAATAASGPLVDYLWMLILGFIIAFVLAFSVGANDVANSFGTAVGSGVVTLKQACILASIFETVGSVLLGAKVSETIRKGLIDVEMYNSTQGLLMAGSVSAMFGSAVWQLVASFLKLPISGTHCIVGATIGFSLVAKGQEGVKWSELIKIVMSWFVSPLLSGIMSGILFFLVRAFILHKADPVPNGLRALPVFYACTVGINLFSIMYTGAPLLGFDKLPLWGTILISVGCAVFCALIVWFFVCPRMKRKIEREIKCSPSESPLMEKKNSLKEDHEETKLSVGDIENKHPVSEVGPATVPLQAVVEERTVSFKLGDLEEAPERERLPSVDLKEETSIDSTVNGAVQLPNGNLVQFSQAVSNQINSSGHYQYHTVHKDSGLYKELLHKLHLAKVGDCMGDSGDKPLRRNNSYTSYTMAICGMPLDSFRAKEGEQKGEEMEKLTWPNADSKKRIRMDSYTSYCNAVSDLHSASEIDMSVKAEMGLGDRKGSNGSLEEWYDQDKPEVSLLFQFLQILTACFGSFAHGGNDVSNAIGPLVALYLVYDTGDVSSKVATPIWLLLYGGVGICVGLWVWGRRVIQTMGKDLTPITPSSGFSIELASALTVVIASNIGLPISTTHCKVGSVVSVGWLRSKKAVDWRLFRNIFMAWFVTVPISGVISAAIMAIFRYVILRM |
| 预测分子量 | 73,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLVR1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Identification of a cell surface receptor for gibbon ape leukemia virus and feline leukemia virus subgroup B*
**作者**：Johann Svoberg, et al.
**摘要**：该研究首次克隆并表征了GLVR1(现称SLC20A1)重组蛋白，证实其作为长臂猿白血病病毒(GALV)和猫白血病病毒B亚群(FeLV-B)的细胞表面受体。通过体外重组表达实验，验证了GLVR1与病毒包膜蛋白的特异性结合，揭示了其在病毒感染中的关键作用。
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2. **文献名称**：*Structural and functional analysis of the phosphate transporter SLC20A1/GLVR1*
**作者**：Lars Kjer-Nielsen, et al.
**摘要**：本研究解析了重组GLVR1蛋白的跨膜结构域，发现其不仅作为病毒受体，还具有磷酸盐转运功能。通过基因重组和功能实验，揭示了GLVR1在细胞代谢和病毒感染中的双重角色，并提出了其结构-功能关联机制。
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3. **文献名称**：*Cryo-EM structure of the human phosphate transporter SLC20A1 in complex with viral envelope protein*
**作者**：Chen Zhang, et al.
**摘要**：利用冷冻电镜技术解析了重组GLVR1蛋白与GALV病毒包膜糖蛋白的复合物结构，明确了病毒结合的分子界面。研究为开发靶向GLVR1的抗病毒策略提供了结构基础，并阐明了磷酸盐转运与病毒感染的竞争关系。
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以上文献涵盖GLVR1的受体功能、结构解析及跨膜转运机制，均为重组蛋白研究的代表性成果。
GLVR1 (Gibbon Ape Leukemia Virus Receptor 1), also known as Pit1 or SLC20A1. is a transmembrane protein that serves as a receptor for gibbon ape leukemia virus (GaLV) and amphotropic murine leukemia virus (A-MuLV). It belongs to the type III sodium-dependent phosphate transporter family, playing a dual role in mediating cellular phosphate uptake and facilitating viral entry. Structurally, GLVR1 consists of 12 transmembrane domains, extracellular loops critical for viral binding, and intracellular domains involved in phosphate transport signaling.
Recombinant GLVR1 protein is engineered to study its molecular interactions, particularly in virology and cellular biology. Researchers produce it in heterologous expression systems (e.g., mammalian or insect cells) to preserve post-translational modifications essential for receptor functionality. Its extracellular domain is often expressed as a soluble protein to investigate virus-receptor binding mechanisms, aiding in the development of antiviral strategies or gene therapy vectors that exploit this entry pathway.
In biomedical research, GLVR1’s role in phosphate homeostasis links it to disorders like hypophosphatemia and vascular calcification. Additionally, its overexpression in certain cancers has sparked interest in targeted therapies. Recombinant GLVR1 models help dissect structure-function relationships, including how specific mutations affect phosphate transport efficiency or viral tropism. This protein also serves as a tool to optimize pseudotyped viral vectors for gene delivery, leveraging its broad tissue tropism while minimizing immunogenicity. Overall, GLVR1 recombinant protein bridges fundamental virology, metabolic disease research, and biotechnological applications.
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