| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPX6 |
| Uniprot No | P59796 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-221aa |
| 氨基酸序列 | Q QTLKPQNRKV DCNKGVTGTI YEYGALTLNG EEYIQFKQFA GKHVLFVNVA AYUGLAAQYP ELNALQEELK NFGVIVLAFP CNQFGKQEPG TNSEILLGLK YVCPGSGFVP SFQLFEKGDV NGEKEQKVFT FLKNSCPPTS DLLGSSSQLF WEPMKVHDIR WNFEKFLVGP DGVPVMHWFH QAPVSTVKSD ILEYLKQFNT H |
| 预测分子量 | 24,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPX6重组蛋白的示例参考文献(注：由于GPX6研究相对较少，以下内容为示例性概括，建议通过学术数据库核实具体文献)：
---
1. **文献名称**: "Cloning, Expression, and Characterization of Recombinant Human GPX6"
**作者**: Smith A, et al.
**摘要**: 该研究成功克隆了人源GPX6基因，并在大肠杆菌系统中表达了重组GPX6蛋白。通过亲和层析纯化后，研究者分析了其酶活性，发现GPX6对特定氢过氧化物底物具有催化活性，但在不同组织中的表达水平差异显著，提示其可能在嗅觉上皮细胞中发挥抗氧化作用。
---
2. **文献名称**: "Functional Analysis of GPX6 in Cellular Antioxidant Defense Systems"
**作者**: Zhang L, et al.
**摘要**: 本文利用哺乳动物细胞系(如HEK293)过表达重组GPX6蛋白，发现其能够显著降低细胞内活性氧(ROS)水平，并增强细胞对氧化应激的抵抗能力。研究还表明，GPX6可能通过硫氧还蛋白系统而非谷胱甘肽系统发挥功能，与其他GPX家族成员存在差异。
---
3. **文献名称**: "Structural Insights into the Catalytic Mechanism of GPX6"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了重组GPX6的三维结构，揭示了其活性中心的关键氨基酸残基(如硒代半胱氨酸)。研究发现GPX6的底物结合口袋具有独特的构象，可能解释其对特定脂质过氧化物的选择性催化能力。
---
4. **文献名称**: "GPX6 Knockout Mouse Model Reveals Its Role in Olfactory Function"
**作者**: Müller R, et al.
**摘要**: 通过构建GPX6基因敲除小鼠，发现缺乏GPX6的小鼠嗅觉神经元更易受到氧化损伤，导致嗅觉功能下降。研究中利用重组GPX6蛋白进行体外补充实验，证实其可恢复神经元抗氧化能力，支持GPX6在嗅觉系统中的保护作用。
---
**注意**：以上文献为示例性内容，实际研究中GPX6相关文献较少，建议结合具体研究方向(如物种、疾病模型等)在PubMed、Web of Science等平台检索最新论文。
GPX6. or Glutathione Peroxidase 6. is a member of the glutathione peroxidase family, a group of antioxidant enzymes that protect cells from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxides using reduced glutathione (GSH). Unlike other GPX isoforms (GPX1-5 and GPX7-8), GPX6 is unique in its tissue-specific expression and evolutionary history. In humans, GPX6 is primarily expressed in the olfactory epithelium and embryonic tissues, suggesting potential roles in sensory function or developmental processes. Phylogenetically, GPX6 shares homology with GPX3 and GPX5 but is distinguished by its selenium-independent catalytic mechanism in some species, relying instead on cysteine residues for enzymatic activity.
Recombinant GPX6 protein is produced through genetic engineering techniques, where the GPX6 gene is cloned into expression vectors (e.g., bacterial, yeast, or mammalian systems) to enable large-scale production. This allows researchers to study its structural and functional properties in vitro. Recombinant GPX6 retains the enzymatic ability to neutralize reactive oxygen species (ROS), making it a valuable tool for investigating oxidative stress-related pathways, cellular redox regulation, and its potential therapeutic applications. Studies have explored its role in mitigating oxidative damage in neurodegenerative diseases, cancer, and inflammatory conditions, though its physiological significance remains less characterized compared to other GPXs.
Research on recombinant GPX6 also focuses on its unique substrate specificity and regulatory mechanisms. Its expression patterns and low abundance in humans pose challenges, necessitating recombinant systems for detailed biochemical analysis. Understanding GPX6 could unveil novel antioxidant strategies or biomarkers for diseases linked to oxidative imbalance.
×
