| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TR2 |
| Uniprot No | P13056 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-603aa |
| 氨基酸序列 | MATIEEIAHQIIEQQMGEIVTEQQTGQKIQIVTALDHNTQGKQFILTNHDGSTPSKVILARQDSTPGKVFLTTPDAAGVNQLFFTTPDLSAQHLQLLTDNSPDQGPNKVFDLCVVCGDKASGRHYGAVTCEGCKGFFKRSIRKNLVYSCRGSKDCIINKHHRNRCQYCRLQRCIAFGMKQDSVQCERKPIEVSREKSSNCAASTEKIYIRKDLRSPLTATPTFVTDSESTRSTGLLDSGMFMNIHPSGVKTESAVLMTSDKAESCQGDLSTLANVVTSLANLGKTKDLSQNSNEMSMIESLSNDDTSLCEFQEMQTNGDVSRAFDTLAKALNPGESTACQSSVAGMEGSVHLITGDSSINYTEKEGPLLSDSHVAFRLTMPSPMPEYLNVHYIGESASRLLFLSMHWALSIPSFQALGQENSISLVKAYWNELFTLGLAQCWQVMNVATILATFVNCLHNSLQQDKMSTERRKLLMEHIFKLQEFCNSMVKLCIDGYEYAYLKAIVLFSPDHPSLENMEQIEKFQEKAYVEFQDYITKTYPDDTYRLSRLLLRLPALRLMNATITEELFFKGLIGNIRIDSVIPHILKMEPADYNSQIIGHSI |
| 预测分子量 | 67,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TR2重组蛋白的3篇参考文献，按简洁格式整理：
1. **文献名称**：Production and Characterization of Recombinant Human TR2 Orphan Receptor
**作者**：Lee, C.H., et al.
**摘要**：该研究报道了人源TR2孤儿核受体的重组表达及功能分析，利用大肠杆菌系统成功纯化TR2蛋白，并验证其DNA结合活性及配体结合特性。
2. **文献名称**：Functional Analysis of TR2 in Stem Cell Regulation via Recombinant Protein Delivery
**作者**：Zhang, Y., et al.
**摘要**：通过哺乳动物细胞表达系统制备TR2重组蛋白，证明其通过Wnt/β-catenin通路调控胚胎干细胞的自我更新能力，为干细胞治疗提供潜在靶点。
3. **文献名称**：Crystal Structure Determination of the TR2 Ligand-Binding Domain Expressed in Insect Cells
**作者**：Tanaka, N., et al.
**摘要**：采用杆状病毒-昆虫细胞系统表达TR2配体结合域，解析其晶体结构，揭示该受体与特定激素反应元件的相互作用机制。
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核对原文准确性。若需扩展，可补充TR2在癌症或代谢疾病中的重组蛋白应用研究。
**Background of TR2 Recombinant Protein**
TR2 recombinant protein, derived from the human TR2 (testicular receptor 2) gene, belongs to the nuclear receptor superfamily, which regulates gene expression in response to hormonal and metabolic signals. TR2. also known as NR2C1. functions as a ligand-dependent transcription factor, though its endogenous ligands remain poorly characterized. It plays roles in diverse biological processes, including cell differentiation, proliferation, and metabolic homeostasis, with notable expression in tissues such as the testis, brain, and liver.
The recombinant form is engineered using biotechnological methods, typically expressed in *E. coli* or mammalian cell systems, to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity protein for research applications. TR2’s recombinant version enables studies on its molecular interactions, DNA-binding properties, and regulatory mechanisms, particularly in pathways linked to cancer, neurodegenerative diseases, and metabolic disorders.
Interest in TR2 has grown due to its dual role as an oncogene or tumor suppressor, depending on cellular context. For example, it may promote prostate cancer progression but inhibit hepatocellular carcinoma growth. Additionally, TR2 interacts with retinoic acid receptors and peroxisome proliferator-activated receptors (PPARs), suggesting crosstalk in lipid metabolism and energy balance.
Despite its potential, challenges persist in elucidating TR2’s precise ligands and tissue-specific functions. Recombinant TR2 serves as a critical tool for drug discovery, aiming to modulate its activity for therapeutic benefits. Ongoing research focuses on identifying synthetic ligands and understanding its epigenetic regulatory network, positioning TR2 as a promising target for treating metabolic syndromes, cancers, and neurological conditions.
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