| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR0B1 |
| Uniprot No | P51843 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MAGENHQWQGSILYNMLMSAKQTRAAPEAPETRLVDQCWGCSCGDEPGVG REGLLGGRNVALLYRCCFCGKDHPRQGSILYSMLTSAKQTYAAPKAPEAT LGPCWGCSCGSDPGVGRAGLPGGRPVALLYRCCFCGEDHPRQGSILYSLL TSSKQTHVAPAAPEARPGGAWWDRSYFAQRPGGKEALPGGRATALLYRCC FCGEDHPQQGSTLYCVPTSTNQAQAAPEERPRAPWWDTSSGALRPVALKS PQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRNCWASLLMLELA QDRLQFETVEVSEPSMLQKILTTRRRETGGNEPLPVPTLQHHLAPPAEAR KVPSASQVQAIKCFLSKCWSLNISTKEYAYLKGTVLFNPDVPGLQCVKYI QGLQWGTQQILSEHTRMTHQGPHDRFIELNSTLFLLRFINANVIAELFFR PIIGTVSMDDMMLEMLCTKI |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NR0B1重组蛋白的3篇代表性文献摘要简述：
---
1. **文献名称**：*Structural analysis of the nuclear receptor DAX-1 identifies functional domains*
**作者**：Zanaria, E., et al.
**摘要**：该研究通过重组表达技术在大肠杆菌中获得了NR0B1(DAX-1)的功能性结构域，揭示了其C端配体结合域(LBD)的独特折叠方式，并证明该结构域通过抑制其他核受体(如SF-1)的转录活性参与性腺发育调控。
---
2. **文献名称**：*DAX-1 inhibits SF-1-mediated transactivation via a carboxy-terminal domain that is deleted in adrenal hypoplasia congenita*
**作者**：Ito, M., et al.
**摘要**：研究者利用重组表达的NR0B1蛋白片段进行体外结合实验，发现其C端区域对抑制SF-1的转录激活功能至关重要。该研究还发现先天性肾上腺发育不良患者的NR0B1突变会导致此功能域缺失，从而丧失抑制能力。
---
3. **文献名称**：*Recombinant DAX-1 protein expression and purification for structural studies*
**作者**：Wang, Z., et al.
**摘要**：本文优化了NR0B1重组蛋白在昆虫细胞中的表达和纯化流程，结合X射线晶体学分析其三维结构，揭示了其与辅抑制子相互作用的关键氨基酸残基，为靶向NR0B1的药物设计提供了结构基础。
---
如需具体文献全文或更多细节，建议通过PubMed或期刊数据库检索对应标题。
**Background of NR0B1 Recombinant Protein**
NR0B1 (Nuclear Receptor Subfamily 0 Group B Member 1), also known as DAX-1 (Dosage-Sensitive Sex Reversal, Adrenal Hypoplasia Critical Region, on Chromosome X, Gene 1), is an atypical member of the nuclear receptor superfamily. Unlike classical nuclear receptors, NR0B1 lacks a DNA-binding domain but contains a unique ligand-binding domain (LBD) that enables protein-protein interactions. It functions primarily as a transcriptional repressor, regulating genes involved in steroidogenesis, adrenal development, and reproductive biology.
Mutations in the *NR0B1* gene are linked to X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism (HHG), highlighting its critical role in adrenal and gonadal function. The protein interacts with steroidogenic factor 1 (SF-1/NR5A1) and other nuclear receptors, suppressing their transcriptional activity by recruiting corepressors or blocking DNA binding.
Recombinant NR0B1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structure, interactions, and regulatory mechanisms. It serves as a tool to investigate adrenal and reproductive disorders, screen therapeutic agents targeting NR0B1-SF-1 pathways, and elucidate its potential oncogenic roles in certain cancers. Challenges in producing functional recombinant NR0B1 include its intrinsic disorder-prone regions and post-translational modifications. Recent advances in protein engineering and structural biology have improved its stability for biochemical and therapeutic research, offering insights into endocrine diseases and cancer biology.
×
