| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AVPI1 |
| Uniprot No | Q5T686 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-147aa |
| 氨基酸序列 | MGTPASVVSE PPPWQAPIEA RGRKQASANI FQDAELLQIQ ALFQRSGDQL AEERAQIIWE CAGDHRVAEA LKRLRRKRPP RQKPLGHSLH HCSRLRILEP HSALANPQSA TETASSEQYL HSRKKSARIR RNWRKSGPTS YLHQIRH |
| 预测分子量 | 16,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AVPI1重组蛋白的3篇参考文献示例(注：AVPI1相关研究较少，部分文献可能与功能相关研究结合)：
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1. **文献名称**: "Expression and purification of recombinant AVPI1 protein in Escherichia coli for functional studies"
**作者**: Kim J, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组AVPI1蛋白，通过亲和层析和尺寸排阻色谱获得高纯度蛋白。Western blot验证其特异性，并证实其能抑制caspase活性，提示其在细胞凋亡调控中的潜在作用。
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2. **文献名称**: "AVPI1 modulates ER stress-induced apoptosis through interaction with Bcl-2 family proteins"
**作者**: Chen L, et al.
**摘要**: 研究发现重组AVPI1蛋白可通过与Bcl-2家族蛋白(如BAX、BCL-XL)相互作用，调节内质网应激诱导的细胞凋亡。体外实验表明，AVPI1过表达显著降低应激条件下细胞死亡率。
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3. **文献名称**: "Structural characterization of AVPI1 recombinant protein and its role in neurodegenerative disease models"
**作者**: Patel R, et al.
**摘要**: 通过X射线晶体学解析了AVPI1重组蛋白的三维结构，揭示了其保守的IAP结构域。在阿尔茨海默病细胞模型中，外源添加重组AVPI1减少了神经元凋亡，表明其神经保护潜力。
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**备注**：
- 若需具体文献，建议进一步核查基因名称准确性(如是否为AVPR1A或AVP相关)，或在PubMed/Google Scholar以“AVPI1 recombinant protein”为关键词检索。
- 上述摘要基于类似研究虚拟生成，实际文献需根据数据库结果调整。
**Background of AVPI1 Recombinant Protein**
AVPI1 (AVEN-Peptide Inhibitor 1) recombinant protein is a synthetic or engineered protein derived from the AVEN gene, which encodes a regulator of apoptosis and cell cycle progression. AVEN functions as an apoptosis inhibitor by binding to Apaf-1 (apoptotic protease-activating factor 1), a key component of the apoptosome, thereby blocking caspase activation and preventing programmed cell death. The AVPI1 peptide mimics a conserved region within AVEN that interacts with Apaf-1. making it a tool to study apoptotic pathways or modulate cell death processes.
Structurally, AVPI1 typically retains the functional BIR (baculovirus IAP repeat)-like domain critical for its anti-apoptotic activity. Recombinant production involves cloning the AVPI1 sequence into expression vectors (e.g., bacterial or mammalian systems) to enable large-scale purification. Tags such as His-tag or GST-tag are often added to facilitate isolation and detection.
Research on AVPI1 focuses on its role in cancer biology, as dysregulated apoptosis is a hallmark of malignancies. By inhibiting apoptosome formation, AVPI1 contributes to tumor cell survival and chemoresistance, making it a potential therapeutic target. Conversely, AVPI1 recombinant protein is also used in experimental settings to antagonize endogenous AVEN activity, aiding in mechanistic studies of apoptosis.
Additionally, AVPI1 has applications in neurodegenerative diseases, where excessive apoptosis is detrimental. Its recombinant form allows for controlled in vitro or in vivo studies to explore cell death pathways and screen for small-molecule inhibitors. Despite its utility, challenges remain in optimizing delivery and specificity for clinical translation. Overall, AVPI1 recombinant protein serves as a versatile tool for dissecting apoptosis regulation and developing targeted therapies.
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