| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COASY |
| Uniprot No | Q13057 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 296-564aa |
| 氨基酸序列 | MASMTGGQQMGRGEFMAINRFRLENDLEELALYQIQLLKDLRHTENEEDK VSSSSFRQRMLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKG LGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRV FGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQ NLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHV VLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COASY重组蛋白的3篇参考文献的简要概括：
1. **"Cloning and functional characterization of human COASY, a bifunctional enzyme involved in coenzyme A synthesis"**
*作者：Dlamini et al.*
摘要：研究报道了人源COASY基因的克隆及其重组蛋白的表达与纯化，证实其具有磷酸泛酸酰半胱氨酸合成酶和脱羧酶的双功能活性，为研究辅酶A合成机制提供基础。
2. **"COASY mutations disrupt coenzyme A metabolism and cause neurodegeneration with brain iron accumulation"**
*作者：Dusi et al.*
摘要：通过重组COASY蛋白功能分析，发现其突变导致酶活性丧失，引发细胞内辅酶A水平下降及铁代谢异常，揭示了COASY缺陷与神经退行性疾病的关联。
3. **"Recombinant COASY protein enhances mitochondrial function in a cellular model of Parkinson’s disease"**
*作者：Santos et al.*
摘要：研究利用重组COASY蛋白处理帕金森病细胞模型，发现其通过恢复辅酶A水平改善线粒体能量代谢，提示COASY在神经保护中的潜在应用价值。
4. **"Structural insights into the catalytic mechanism of the bifunctional enzyme COASY"**
*作者：Zhang et al.*
摘要：通过解析重组COASY蛋白的晶体结构，揭示了其双功能结构域的协同作用机制，为设计靶向COASY的小分子药物提供结构基础。
(注：上述文献为示例性内容，实际引用时需核实具体文献信息。)
COASY (Coenzyme A Synthase) is a bifunctional enzyme critical for the final steps of coenzyme A (CoA) biosynthesis, an essential cofactor involved in numerous metabolic pathways, including fatty acid oxidation, the tricarboxylic acid (TCA) cycle, and neurotransmitter synthesis. COASY catalyzes two sequential reactions: phosphorylation of pantothenate to form 4'-phosphopantothenate (via its phosphopantothenate domain) and subsequent ligation with cysteine to synthesize coenzyme A (via its synthetase domain). Dysregulation of COASY has been linked to neurodegenerative disorders, mitochondrial dysfunction, and cancer, highlighting its physiological importance.
Recombinant COASY protein, produced through heterologous expression systems like *E. coli* or mammalian cell cultures, enables detailed biochemical and structural studies. Its recombinant form is engineered to retain enzymatic activity while offering high purity and scalability, facilitating drug discovery and mechanistic research. For instance, recombinant COASY is used to investigate CoA deficiency-related diseases, screen potential enzyme inhibitors or activators, and explore its role in cellular energy homeostasis. Additionally, structural studies using recombinant COASY have provided insights into its catalytic mechanisms and interaction with substrates, aiding the design of targeted therapies. As CoA metabolism gains attention in metabolic disorders and cancer, recombinant COASY remains a vital tool for advancing both basic science and therapeutic applications.
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