| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCCb |
| Uniprot No | P05166 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 7-266aa |
| 氨基酸序列 | VAAVGARLSVLASGLRAAVRSLCSQATSVNERIENKRRTALLGGGQRRIDAQHKRGKLTARERISLLLDPGSFVESDMFVEHRCADFGMAADKNKFPGDSVVTGRGRINGRLVYVFSQDFTVFGGSLSGAHAQKICKIMDQAITVGAPVIGLNDSGGARIQEGVESLAGYADIFLRNVTASGVIPQISLIMGPCAGGAVYSPALTDFTFMVKDTSYLFIT |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCCb(假设为丙酰辅酶A羧化酶β亚基)重组蛋白研究的示例性参考文献，仅供参考。实际文献需通过学术数据库检索获取：
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1. **《重组人丙酰辅酶A羧化酶β亚基的表达与功能分析》**
- 作者：Zhang Y, et al.
- 摘要：研究利用大肠杆菌系统成功表达并纯化重组人PCCb蛋白，证实其具有体外酶活性，为酶缺陷相关疾病(如丙酸血症)的治疗提供实验基础。
2. **《丙酰辅酶A羧化酶β亚基的结构解析与重组表达优化》**
- 作者：Johnson R, et al.
- 摘要：通过基因工程技术优化PCCb的重组表达条件，结合X射线晶体学解析其三维结构，揭示关键催化位点，助力靶向药物设计。
3. **《基于哺乳动物细胞的重组PCCb蛋白在代谢疾病模型中的应用》**
- 作者：Lee S, et al.
- 摘要：在HEK293细胞中高效表达功能性重组PCCb蛋白，验证其在丙酸血症小鼠模型中改善代谢异常的效果，推动酶替代疗法的临床前研究。
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**提示**：实际研究中建议以“Propionyl-CoA carboxylase beta subunit recombinant”为关键词，在PubMed、Google Scholar等平台检索，并筛选近年高相关性文献。
**Background of PCCβ Recombinant Protein**
PCCβ (Propionyl-CoA Carboxylase beta subunit) is a critical enzyme subunit involved in the mitochondrial catabolism of branched-chain amino acids, cholesterol, and fatty acids. It forms a heterodimeric complex with the alpha subunit (PCCα) to constitute propionyl-CoA carboxylase (PCC), a biotin-dependent enzyme essential for propionate metabolism. PCC catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA, a key step in the tricarboxylic acid (TCA) cycle. Defects in PCCβ, encoded by the *PCCB* gene, are linked to propionic acidemia, an autosomal recessive metabolic disorder characterized by life-threatening acidosis and multi-organ dysfunction.
Recombinant PCCβ protein is engineered to study its structure-function relationships, disease mechanisms, and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), it retains enzymatic activity when assembled with PCCα, enabling in vitro analysis of PCC function. Researchers utilize recombinant PCCβ to investigate mutations causing propionic acidemia, screen small-molecule chaperones to restore enzyme activity, and develop enzyme replacement therapies (ERT) or gene therapies.
Recent advances in protein engineering and structural biology (e.g., cryo-EM) have elucidated PCCβ's role in substrate binding and biotin coordination, aiding drug design. Recombinant PCCβ also serves as a diagnostic antigen or reference material in clinical assays. Despite challenges in stabilizing the large, multi-domain protein, ongoing studies aim to optimize its production and delivery for translational applications, offering hope for targeted treatments for propionic acidemia and related metabolic disorders.
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