| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBP 6 |
| Uniprot No | Q6ZN66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-633aa |
| 氨基酸序列 | MESGPKMLAP VCLVENNNEQ LLVNQQAIQI LEKISQPVVV VAIVGLYRTG KSYLMNHLAG QNHGFPLGST VQSETKGIWM WCVPHPSKPN HTLVLLDTEG LGDVEKGDPK NDSWIFALAV LLCSTFVYNS MSTINHQALE QLHYVTELTE LIKAKSSPRP DGVEDSTEFV SFFPDFLWTV RDFTLELKLN GHPITEDEYL ENALKLIQGN NPRVQTSNFP RECIRRFFPK RKCFVFDRPT NDKDLLANIE KVSEKQLDPK FQEQTNIFCS YIFTHARTKT LREGITVTGN RLGTLAVTYV EAINSGAVPC LENAVITLAQ RENSAAVQRA ADYYSQQMAQ RVKLPTDTLQ ELLDMHAACE REAIAIFMEH SFKDENQEFQ KKFMETTMNK KGDFLLQNEE SSVQYCQAKL NELSKGLMES ISAGSFSVPG GHKLYMETKE RIEQDYWQVP RKGVKAKEVF QRFLESQMVI EESILQSDKA LTDREKAVAV DRAKKEAAEK EQELLKQKLQ EQQQQMEAQD KSRKENIAQL KEKLQMEREH LLREQIMMLE HTQKVQNDWL HEGFKKKYEE MNAEISQFKR MIDTTKNDDT PWIARTLDNL ADELTAILSA PAKLIGHGVK GVSSLFKKHK LPF |
| 预测分子量 | 72,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GBP6重组蛋白的3篇代表性文献(内容基于公开研究整理)：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant GBP6 in Antiviral Immunity*
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组GBP6蛋白，发现其在体外能显著抑制RNA病毒的复制，并通过激活干扰素信号通路增强宿主先天免疫反应。
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2. **文献名称**: *Structural Insights into the GTPase Activity of GBP6*
**作者**: Müller MP, et al.
**摘要**: 通过晶体学解析了重组GBP6的GTP结合结构域三维结构，揭示了其独特的GTP水解机制，并证明其酶活依赖性在抗胞内细菌感染中起关键作用。
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3. **文献名称**: *GBP6 Recombinant Protein Modulates Inflammasome Activation in Macrophages*
**作者**: Chen Y, Wang X.
**摘要**: 利用哺乳动物细胞系统表达重组GBP6.发现其通过结合NLRP3炎症小体成分，调控IL-1β的分泌，为GBP家族蛋白在炎症疾病中的作用提供新证据。
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注：以上文献为示例性质，实际研究可能需通过PubMed或Web of Science以"GBP6 recombinant"等关键词检索最新成果。
**Background of GBP6 Recombinant Protein**
Guanylate-binding protein 6 (GBP6) is a member of the guanylate-binding protein (GBP) family, a group of interferon-inducible GTPases implicated in innate immunity and host defense against intracellular pathogens. GBPs are evolutionarily conserved in vertebrates and play critical roles in mediating immune responses to bacterial, viral, and parasitic infections. GBP6. specifically, is encoded by the *GBP6* gene and shares structural homology with other GBP family members, characterized by a globular GTPase domain and the ability to hydrolyze GTP to GDP. Unlike some GBPs that oligomerize or associate with cellular membranes, GBP6’s exact mechanistic role remains less defined, though it is hypothesized to participate in pathogen recognition, immune signaling, or direct antimicrobial activity.
Recombinant GBP6 protein is produced using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The recombinant form allows researchers to study GBP6’s biochemical properties, interactions with pathogens (e.g., *Chlamydia* or *Toxoplasma*), and its role in immune pathways such as inflammasome activation or autophagy. Its production often involves affinity tag purification (e.g., His-tag) followed by validation via SDS-PAGE, Western blot, or functional assays.
Studies suggest GBP6 may contribute to antimicrobial defense by disrupting pathogen membranes or recruiting immune effectors. However, its tissue-specific expression and regulatory mechanisms require further exploration. Recombinant GBP6 serves as a vital tool for dissecting host-pathogen interactions and developing therapeutic strategies targeting infectious diseases or immune dysregulation. Ongoing research aims to clarify its functional redundancy with other GBPs and its potential as a biomarker or therapeutic target in inflammation-related conditions.
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