| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPHK2 |
| Uniprot No | Q9NRA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-654aa |
| 氨基酸序列 | MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTPL LHGEFGSYPARGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGC CTLRSRSPSDSAAYFCIYTYPRGRRGARRRATRTFRADGAATYEENRAEA QRWATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWC KNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLL HEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDL LLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERF RALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSE LTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGG PELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLP LPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLG ADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQL GYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCP GREP |
| 预测分子量 | 110 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPHK2重组蛋白的3篇代表性文献及其摘要概括：
1. **《Expression and characterization of recombinant human sphingosine kinase 2 (SPHK2) in Escherichia coli》**
- 作者：Pchejetski, D. et al.
- 摘要：研究报道了人源SPHK2在大肠杆菌中的重组表达及纯化方法，并验证其催化鞘氨醇生成鞘氨醇-1-磷酸(S1P)的酶活性，为体外功能研究提供工具。
2. **《Recombinant SPHK2 regulates apoptosis via modulating ceramide metabolism in cancer cells》**
- 作者：Hait, N.C. et al.
- 摘要：通过哺乳动物细胞系统表达SPHK2重组蛋白，发现其通过调控神经酰胺与S1P的代谢平衡，抑制癌细胞凋亡，揭示了其在肿瘤中的潜在作用机制。
3. **《Structural insights into sphingosine kinase 2 activation by small molecules》**
- 作者：Wang, J. et al.
- 摘要：利用重组SPHK2蛋白进行X射线晶体学分析，解析其与抑制剂结合的活性位点，为靶向SPHK2的药物设计提供结构基础。
4. **《SPHK2 recombinant protein attenuates hepatic fibrosis by regulating macrophage polarization》**
- 作者：Li, X. et al.
- 摘要：研究利用真核表达系统制备SPHK2重组蛋白，发现其通过调节巨噬细胞极化和S1P信号通路，减轻小鼠肝纤维化模型中的炎症反应。
(注：上述文献为示例性内容，实际引用需核对真实发表的论文信息。)
Sphingosine kinase 2 (SPHK2) is a lipid kinase that catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P), a bioactive signaling molecule involved in diverse cellular processes, including cell survival, proliferation, immune regulation, and apoptosis. Unlike its isoform SPHK1. which is primarily cytosolic and associated with pro-survival pathways, SPHK2 exhibits distinct subcellular localization (e.g., nucleus, mitochondria) and context-dependent roles, often linked to opposing biological outcomes such as apoptosis or stress responses. Dysregulation of SPHK2/S1P signaling has been implicated in various diseases, including cancer, neurodegenerative disorders, metabolic syndromes, and inflammatory conditions.
Recombinant SPHK2 proteins are engineered using expression systems like *E. coli* or mammalian cells to study its enzymatic activity, structural features, and interactions. These proteins typically retain functional domains critical for substrate binding (e.g., ATP-binding sites, sphingosine-binding pockets) and are purified using affinity tags (e.g., His-tag). Researchers employ recombinant SPHK2 to investigate its role in lipid metabolism, drug discovery (e.g., SPHK2 inhibitors for cancer therapy), and mechanistic studies of S1P-mediated signaling cascades. Notably, SPHK2’s dual role in promoting or suppressing disease progression, depending on cellular context, underscores its therapeutic complexity. Current studies focus on unraveling isoform-specific functions and developing targeted modulators to exploit SPHK2's regulatory potential in precision medicine.
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