| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRMP5 |
| Uniprot No | Q9BPU6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-564aa |
| 氨基酸序列 | MLANSASVRILIKGDKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKV IDATGKLVIPGGIDTSTHFHQTFMNATCVDDFYHGTKAALVGGTTMIIGH VLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPKVKAEMETLVR EKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAE GAKEALDLGITGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSS ISAGDVIAAAKMQGKVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPL RLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSG VQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADA DVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENG VFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVV HPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPERASA RILAPPGGRSSGIW |
| 预测分子量 | 88 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CRMP5重组蛋白的3篇代表性文献(基于已有研究方向的模拟示例)：
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1. **文献名称**：*Autoantibodies to CRMP5 in paraneoplastic neurological syndromes: Purification and characterization of recombinant CRMP5 antigen*
**作者**：Yu et al.
**摘要**：本研究利用大肠杆菌表达系统制备了重组CRMP5蛋白，并通过免疫印迹和ELISA验证其在副肿瘤综合征患者血清中的自身抗体结合活性，证实其作为临床诊断标志物的潜力。
2. **文献名称**：*CRMP5 promotes axonal growth via interaction with tubulin heterodimers*
**作者**：Fukata et al.
**摘要**：通过体外重组CRMP5蛋白与微管蛋白的结合实验，揭示了CRMP5在轴突导向中通过调控微管组装促进神经元极性建立的分子机制。
3. **文献名称**：*Structural insights into CRMP5 function by X-ray crystallography of the recombinant protein*
**作者**：Chaudhry et al.
**摘要**：首次解析了重组CRMP5蛋白的晶体结构，阐明其四聚体构象及关键功能域，为靶向CRMP5的神经退行性疾病药物设计提供结构基础。
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*注：以上文献为模拟示例，实际引用时需以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“CRMP5 recombinant”检索最新研究。*
**Background of CRMP5 Recombinant Protein**
Collapsin Response Mediator Protein 5 (CRMP5), also known as CRMP-5 or DRP-5. is a member of the CRMP family of cytosolic phosphoproteins predominantly expressed in the nervous system. These proteins are crucial for neuronal development, including axon guidance, dendritic branching, and neuronal polarity. CRMP5. encoded by the *DPYSL5* gene, shares structural homology with other CRMPs, featuring an N-terminal domain involved in tubulin binding and a C-terminal region critical for interactions with signaling molecules.
CRMP5 regulates microtubule dynamics and growth cone collapse by interacting with cytoskeletal components and signaling pathways, such as the Semaphorin 3A (Sema3A)-neuropilin-1/plexin-A4 axis. Dysregulation of CRMP5 is implicated in neurological disorders, including Alzheimer’s disease, neuropathic pain, and paraneoplastic syndromes, where autoantibodies against CRMP5 are associated with encephalomyelitis and sensory neuropathy.
Recombinant CRMP5 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity protein for functional studies. This recombinant tool enables researchers to investigate CRMP5’s role in neuronal signaling, screen for autoantibodies in clinical diagnostics, and explore therapeutic targets for neuropathologies. Its application extends to studying molecular mechanisms of neurodegeneration and developing biomarkers for autoimmune neurological diseases.
In summary, CRMP5 recombinant protein serves as a vital resource for dissecting neural development pathways and disease mechanisms, bridging basic research and clinical applications.
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