| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GBP 2 |
| Uniprot No | P32456 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-588aa |
| 氨基酸序列 | MAPEINLPGP MSLIDNTKGQ LVVNPEALKI LSAITQPVVV VAIVGLYRTG KSYLMNKLAG KKNGFSLGS TVKSHTKGIW MWCVPHPKKP EHTLVLLDTE GLGDIEKGDN ENDSWIFALA ILLSSTFVY NSMGTINQQA MDQLHYVTEL TDRIKANSSP GNNSVDDSAD FVSFFPAFVW TLRDFTLEL EVDGEPITAD DYLELSLKLR KGTDKKSKSF NDPRLCIRKF FPKRKCFVFD WPAPKKYLA HLEQLKEEEL NPDFIEQVAE FCSYILSHSN VKTLSGGIPV NGPRLESLVL TYVNAISSG DLPCMENAVL ALAQIENSAA VEKAIAHYEQ QMGQKVQLPT ETLQELLDLH RDSEREAIE VFMKNSFKDV DQMFQRKLGA QLEARRDDFC KQNSKASSDC CMALLQDIFG PLEEDVKQG TFSKPGGYRL FTQKLQELKN KYYQVPRKGI QAKEVLKKYL ESKEDVADAL LQTDQSLSE KEKAIEVERI KAESAEAAKK MLEEIQKKNE EMMEQKEKSY QEHVKQLTEK MERDRAQLM AEQEKTLALK LQEQERLLKE GFENESKRLQ KDIWDIQMRS KSLEPIC |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GBP2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *"Recombinant expression and functional characterization of human guanylate-binding protein 2 (GBP2) in antiviral immunity"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中重组表达了人源GBP2蛋白，并验证其GTP酶活性。实验表明，重组GBP2通过形成多聚体结构抑制病毒复制，揭示了其在干扰素信号通路中的直接抗病毒作用。
2. **文献名称**: *"Structural insights into the mechanism of GBP2 oligomerization and pathogen membrane targeting"*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组GBP2的晶体结构，发现其C端结构域介导了蛋白寡聚化及与病原体膜的结合。该研究为GBP2在宿主防御中破坏病原体膜的分子机制提供了结构基础。
3. **文献名称**: *"GBP2 inflammasome activation in response to intracellular bacteria requires recombinant protein dimerization"*
**作者**: Gupta R, et al.
**摘要**: 研究利用重组GBP2蛋白证明，其通过二聚化激活NLRP3炎症小体，促进白细胞介素-1β释放。该发现阐明了GBP2在细胞自主免疫中对胞内细菌感染的应答机制。
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以上文献涵盖了GBP2重组蛋白的表达、结构功能及免疫机制研究，均为假设性示例，实际引用需根据具体文献调整。
**Background of GBP2 Recombinant Protein**
Guanylate-binding protein 2 (GBP2) is a member of the dynamin-related guanylate-binding protein (GBP) family, which is interferon-inducible and plays critical roles in innate immunity. GBPs are large GTPases that hydrolyze GTP to both GDP and GMP, a unique enzymatic feature distinguishing them from other GTPases. GBP2 is primarily induced by type I and II interferons during inflammatory responses, positioning it as a key mediator in host defense against intracellular pathogens, including bacteria, viruses, and parasites.
Structurally, GBP2 contains an N-terminal GTPase domain and a C-terminal helical domain, enabling oligomerization and membrane binding. These features facilitate its function in pathogen membrane disruption, inflammasome activation, and coordination of antimicrobial effector mechanisms. Studies highlight GBP2’s role in targeting pathogen-containing vacuoles (e.g., *Salmonella*), promoting vacuolar rupture and exposing pathogens to cytosolic immune sensors. Additionally, GBP2 collaborates with other GBPs, such as GBP1 and GBP5. to amplify immune responses and restrict pathogen replication.
Recombinant GBP2 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells*) to study its biochemical properties, interactions, and therapeutic potential. Purified recombinant GBP2 enables structural analysis (e.g., crystallography), enzymatic activity assays, and investigation of its immunomodulatory functions in vitro and in vivo. Dysregulation of GBP2 has been implicated in autoimmune diseases and cancer, underscoring its dual role in immunity and pathology.
Current research focuses on deciphering GBP2’s molecular mechanisms in pathogen defense and its interplay with other immune components. Recombinant GBP2 remains a vital tool for exploring its therapeutic applications, including antimicrobial strategies and immune modulation.
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