| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCM3 |
| Uniprot No | P25205 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-808aa |
| 氨基酸序列 | MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI |
| 预测分子量 | 90,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MCM3重组蛋白的3篇参考文献，涵盖其表达、功能及结构研究：
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1. **文献名称**：*"Recombinant expression and functional characterization of the human MCM3 protein in Saccharomyces cerevisiae"*
**作者**：Smith A, et al.
**摘要**：研究报道了人源MCM3重组蛋白在酵母系统中的成功表达与纯化，并通过遗传互补实验验证其功能，证明重组MCM3能够恢复酵母MCM3缺陷株的DNA复制能力，支持其在复制起始中的作用。
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2. **文献名称**：*"Structural insights into the ATPase activity of MCM3 subunit in the eukaryotic replisome"*
**作者**：Tanaka H, et al.
**摘要**：通过X射线晶体学解析了重组MCM3蛋白的ATP结合结构域，揭示了其ATP水解活性的分子机制，并提出其在复制叉解旋酶复合体中的构象变化调控模型。
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3. **文献名称**：*"Biochemical characterization of recombinant MCM3-MCM5 heterodimer and its role in replication licensing"*
**作者**：Li Y, et al.
**摘要**：研究利用大肠杆菌共表达系统制备了MCM3-MCM5重组异源二聚体，通过体外实验证明该复合体与ORC(起始识别复合物)结合，促进染色质上的复制许可信号传导。
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(注：以上文献标题及内容为示例，实际引用需根据具体论文调整。)
**Background of MCM3 Recombinant Protein**
MCM3 (Minichromosome Maintenance Complex Component 3) is a crucial protein involved in DNA replication, belonging to the conserved MCM family (MCM2-7). These proteins form a hexameric ring structure essential for the initiation and elongation phases of DNA replication in eukaryotes. Specifically, the MCM2-7 complex acts as a replicative DNA helicase, unwinding double-stranded DNA at replication origins during the S phase of the cell cycle. MCM3. as a core subunit, contributes to the ATPase activity and structural stability of the helicase, ensuring proper DNA unwinding and replication fork progression.
Recombinant MCM3 is produced using genetic engineering techniques, often expressed in *E. coli* or insect cell systems (e.g., baculovirus), followed by purification via affinity chromatography. This engineered protein retains biological activity, enabling researchers to study its role in DNA replication mechanisms, cell cycle regulation, and genome stability. Its applications span *in vitro* assays (e.g., helicase activity tests), protein-protein interaction studies, and investigations into replication origin licensing.
Dysregulation of MCM3 is linked to cancer and developmental disorders, as overexpression is observed in rapidly proliferating cells. Consequently, recombinant MCM3 serves as a valuable tool for exploring oncogenesis and identifying therapeutic targets. Recent studies also highlight its potential in understanding replication stress responses and chromatin remodeling.
Overall, MCM3 recombinant protein is pivotal for dissecting the molecular basis of DNA replication and its implications in disease, bridging structural biology with translational research.
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