| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WARS2 |
| Uniprot No | Q9UGM6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-360aa |
| 氨基酸序列 | LH KGSAAAPALQ KDSKKRVFSG IQPTGILHLG NYLGAIESWV RLQDEYDSVL YSIVDLHSIT VPQDPAVLRQ SILDMTAVLL ACGINPEKSI LFQQSQVSEH TQLSWILSCM VRLPRLQHLH QWKAKTTKQK HDGTVGLLTY PVLQAADILL YKSTHVPVGE DQVQHMELVQ DLAQGFNKKY GEFFPVPESI LTSMKKVKSL RDPSAKMSKS DPDKLATVRI TDSPEEIVQK FRKAVTDFTS EVTYDPAGRA GVSNIVAVHA AVTGLSVEEV VRRSAGMNTA RYKLAVADAV IEKFAPIKRE IEKLKLDKDH LEKVLQIGSA KAKELAYTVC QEVKKLVGFL |
| 预测分子量 | 40,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WARS2重组蛋白的3篇代表性文献概览(注：文献为模拟示例，实际引用需核实数据库)：
1. **《Structural and functional analysis of recombinant human WARS2 protein》**
- **作者**: Smith A, et al.
- **摘要**: 本研究通过大肠杆菌系统表达并纯化了重组人源WARS2蛋白，解析了其晶体结构，揭示了其与线粒体tRNA结合的关键结构域，并验证了其氨基酰化酶活性，为研究WARS2突变相关疾病的分子机制提供基础。
2. **《WARS2 deficiency in Drosophila models links mitochondrial dysfunction to neurodevelopmental disorders》**
- **作者**: Chen L, et al.
- **摘要**: 利用重组WARS2蛋白在果蝇模型中回补基因缺陷，证明WARS2功能丧失导致线粒体呼吸链异常和神经元凋亡，提示其在神经发育障碍中的病理作用，重组蛋白可部分恢复表型。
3. **《Therapeutic potential of recombinant WARS2 in mitochondrial encephalomyopathy》**
- **作者**: Kim HJ, et al.
- **摘要**: 在携带WARS2突变的患者来源细胞中，外源性重组WARS2蛋白通过腺病毒载体递送，显著改善线粒体翻译缺陷和ATP合成，为基因治疗策略提供实验依据。
**建议**：实际研究中可通过PubMed或Web of Science以“WARS2 recombinant”“WARS2 mitochondrial disease”为关键词检索最新文献，重点关注蛋白功能、疾病模型及治疗方向的论文。
**Background of WARS2 Recombinant Protein**
The WARS2 gene encodes mitochondrial tryptophanyl-tRNA synthetase (mtTrpRS), an enzyme critical for mitochondrial protein synthesis. As a member of the aminoacyl-tRNA synthetase family, WARS2 catalyzes the attachment of tryptophan to its cognate tRNA, ensuring the accuracy of mitochondrial translation. This process is essential for the production of mitochondrial-encoded proteins involved in oxidative phosphorylation (OXPHOS), the primary pathway for cellular ATP generation.
Mutations in WARS2 are linked to mitochondrial disorders, including early-onset Parkinsonism, intellectual disability, and neurodegenerative conditions. These pathologies highlight the enzyme’s role in maintaining mitochondrial function, particularly in energy-demanding tissues like the brain and muscle.
Recombinant WARS2 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional version of the enzyme. This recombinant form retains the ability to bind ATP, recognize tRNA, and catalyze aminoacylation, enabling its use in biochemical and structural studies. Researchers employ it to investigate disease mechanisms, screen therapeutic compounds, or develop gene-editing tools targeting WARS2-related disorders.
Additionally, recombinant WARS2 serves as a tool to explore mitochondrial translation dynamics and its crosstalk with cellular stress responses. Its study may offer insights into broader mitochondrial dysfunction seen in aging, metabolic diseases, and cancer. Despite progress, challenges remain in optimizing stability and delivery for therapeutic applications, underscoring the need for continued research on this multifunctional protein.
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