首页 / 产品 / 蛋白 / 跨膜蛋白

Recombinant Human ABCA7 protein

  • 中文名: ATP结合盒转运蛋白A7(ABCA7)重组蛋白
  • 别    名: ABCA7;Phospholipid-transporting ATPase ABCA7
货号: PA2000-949DB
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ABCA7
Uniprot No Q8IZY2
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间全长
氨基酸序列full
预测分子量234,3 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ABCA7重组蛋白的3篇代表性参考文献示例(内容基于研究领域常见主题,具体文献信息需进一步验证):

---

1. **文献名称**: *"Functional characterization of ABCA7 recombinant protein in lipid transport"*

**作者**: Kim WS, et al.

**摘要**: 本研究在HEK293细胞中表达了ABCA7重组蛋白,证实其通过ATP依赖的方式促进磷脂和胆固醇的跨膜转运,并揭示了其在细胞膜脂质稳态中的潜在作用。

2. **文献名称**: *"ABCA7 deficiency accelerates amyloid-β generation in Alzheimer's disease mouse models"*

**作者**: Satoh K, et al.

**摘要**: 通过重组ABCA7蛋白的功能实验,发现其缺失会导致神经元中β-淀粉样蛋白(Aβ)的异常积累,提示ABCA7可能通过调节脂质代谢参与阿尔茨海默病的病理过程。

3. **文献名称**: *"Purification and structural analysis of human ABCA7 transmembrane domain"*

**作者**: Abe-Dohmae S, et al.

**摘要**: 利用昆虫细胞系统表达并纯化ABCA7重组蛋白的跨膜结构域,结合冷冻电镜技术解析其三维结构,为理解其脂质转运机制提供结构基础。

---

**注意**:以上为示例,实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“ABCA7 recombinant protein expression”或“ABCA7 lipid Alzheimer”查找最新研究。

背景信息

ABCA7 (ATP-binding cassette subfamily A member 7) is a transmembrane protein encoded by the ABCA7 gene, belonging to the ABC transporter superfamily known for ATP-dependent lipid transport across cellular membranes. Predominantly expressed in the brain, immune cells, and tissues with high lipid metabolism, ABCA7 plays roles in lipid homeostasis, phagocytosis, and clearance of cellular debris. Its structural features include two transmembrane domains and two nucleotide-binding domains, which facilitate substrate binding and energy-driven transport.

Research links ABCA7 to Alzheimer's disease (AD), with genome-wide studies identifying ABCA7 variants as significant risk factors. The protein is implicated in amyloid-beta metabolism, as it may regulate the clearance of amyloid-beta peptides—a hallmark of AD pathology. However, its exact mechanism remains debated; some studies suggest it promotes amyloid-beta generation via phospholipid redistribution, while others propose protective roles through phagocytic clearance or apolipoprotein-mediated lipid transport.

Recombinant ABCA7 proteins are engineered to study its biochemical properties and disease relevance. Produced via heterologous expression systems (e.g., mammalian cells, insect cells), these proteins retain functional domains for in vitro assays, such as lipid-binding studies, ATPase activity measurements, or interaction screens with amyloid-beta or apolipoproteins. Challenges include maintaining structural integrity due to its large size (>2.000 amino acids) and complex post-translational modifications. Current applications span drug discovery (e.g., screening modulators of lipid transport), antibody development for diagnostic tools, and mechanistic studies to resolve its dual roles in AD pathogenesis. Understanding ABCA7's functions through recombinant models could unveil therapeutic strategies targeting lipid dysregulation or protein aggregation in neurodegenerative diseases.

客户数据及评论

折叠内容

大包装询价

×