| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFC2 |
| Uniprot No | P35250 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MEVEAVCGGA GEVEAQDSDP APAFSKAPGS AGHYELPWVE KYRPVKLNEI VGNEDTVSRL EVFAREGNVP NIIIAGPPGT GKTTSILCLA RALLGPALKD AMLELNASND RGIDVVRNKI KMFAQQKVTL PKGRHKIIIL DEADSMTDGA QQALRRTMEI YSKTTRFALA CNASDKIIEP IQSRCAVLRY TKLTDAQILT RLMNVIEKER VPYTDDGLEA IIFTAQGDMR QALNNLQSTF SGFGFINSEN VFKVCDEPHP LLVKEMIQHC VNANIDEAYK ILAHLWHLGY SPEDIIGNIF RVCKTFQMAE YLKLEFIKEI GYTHMKIAEG VNSLLQMAGL LARLCQKTMA PVAS |
| 预测分子量 | 39,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFC2重组蛋白的3篇参考文献示例(注：以下信息基于领域内典型研究方向的模拟描述，实际文献需通过学术数据库核实)：
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1. **"Reconstitution of Human Replication Factor C Subunits and Characterization of RFC2 ATPase Activity"**
*作者：Ellison, V., & Stillman, B. (1998)*
**摘要**：通过重组表达纯化人源RFC复合体各亚基(包括RFC2)，证实RFC2的ATP酶活性对PCNA的加载及DNA复制起始至关重要。
2. **"RFC2-Mediated Checkpoint Control in Response to DNA Damage Requires Interaction with Rad17"**
*作者：Tsuruga, H., et al. (2007)*
**摘要**：利用重组RFC2蛋白进行体外结合实验，揭示RFC2与Rad17的互作在DNA损伤后激活细胞周期检查点中的调控机制。
3. **"Cryo-EM Structure of RFC2-Containing Replication Complex Reveals Conformational Dynamics"**
*作者：Zhang, Z., et al. (2020)*
**摘要**：基于重组RFC2蛋白的冷冻电镜结构解析，阐明RFC2在DNA复制过程中协调复制解旋酶与PCNA环结合的构象变化。
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**提示**：建议通过PubMed或Google Scholar检索关键词“RFC2 recombinant”、“RFC2 subunit replication”等获取具体文献，并优先选择高被引研究或权威期刊论文。
RFC2 (Replication Factor C Subunit 2) is a critical component of the replication factor C (RFC) complex, a conserved AAA+ ATPase family protein essential for DNA replication and repair in eukaryotic cells. The RFC complex functions as a clamp loader, facilitating the ATP-dependent assembly of proliferating cell nuclear antigen (PCNA) onto DNA, which stabilizes DNA polymerase processivity during replication and coordinates repair machinery during DNA damage response. RFC2. as one of the five RFC subunits (RFC1-5), plays a structural and regulatory role in the complex. While RFC1 (the large subunit) binds ATP, RFC2-5 form a core subcomplex responsible for ATP hydrolysis and conformational changes required for PCNA loading.
Recombinant RFC2 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), retains these functional properties and is widely used to study RFC complex dynamics. Its recombinant form enables precise biochemical characterization, including interactions with other RFC subunits, DNA, and PCNA. Research has highlighted RFC2's involvement in cell cycle regulation, particularly at the G1/S transition, and its dysregulation in cancers, where altered RFC2 expression correlates with genomic instability and tumor progression. Additionally, recombinant RFC2 serves as a tool for screening anticancer drugs targeting DNA replication machinery. Structural studies using recombinant protein have revealed conserved domains critical for ATPase activity and subunit interactions, providing insights into clamp loader mechanisms across species. Current investigations also explore its role in non-canonical processes like telomere maintenance and epigenetic regulation, expanding its significance beyond classical replication roles.
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